We are happy to share our new publication about a modified version of PD-1 receptor, or how to turn something negative to an enhancing molecule. There have been a few reports this year showing technologies similar to ours, suggesting that interest for associated molecules to increase the binding might represent a solution to improve cell -based therapy. We thank all co-authors and of course our support: Helse Sør Øst, The Research Council of Norway and The Norwegian Cancer Society

Paper 1:

Targeting KRAS mutations with HLA class II-restricted TCRs for the treatment of solid tumors

Paper 2:

Circulating Tumor Cell Persistence Associates with Long-TermClinical Outcome to a Therapeutic Cancer Vaccine inProstate Cancer

Ingrid Jenny Guldvik, Lina Ekseth, Amar U. Kishan, Andreas Stensvold, Else Marit Inderberg and Wolfgang Lilleby

We are very proud of Synne Halvorsen Hougsnæs who succesfully defended her Master of Science in Biotechnology:

"CMV-specific Immune Responses Induced by Dendritic Cell Vaccines in Glioblastoma patients
And Clinical Benefit"

The normal protocol was respected:

Defense                                                                       Flowers                                                         Cake

Anna Winge-Main, who is medical oncologist (melanoma), but also PhD student in our unit has been interviewed (here) to give an overview of the melanoma studies presented in ASCO-2021:

Clara has successfully defended her Master thesis today and will join us next week.

We now know that one should be careful when selecting LB medium.

Credit: C. Forcados laptop at OCCI social area

Congratulations to Anna Winge-Main, PhD student in our unit and MD at the Department of Oncology (OUS), who has published a case report in the British Medical Journal (Case Reports):

Our method to clone CAR from hybridoma is finally cited in Pubmed:

More impotrantly it also present the first anti-Iglambda CAR:

Anna Winge-Main speaks at the session “Navigating Early-Stage Melanoma: Systemic Treatment Today and in the Future?” at the 10th World Congress of Melanoma/17th EADO Congress Industry Satellite Symposium 15^th of April 2021

https://worldmelanoma2021.com//files/2021/04/28-Final-Programme-WCM-2021.pdf

Congratulations to our collaborator and friend Andres Maturana for his last publication integrating some of our design (NB: this is not immunotherapy!)

More information here

Find out more about this excellent training program of ADVANCE 

Credit: ADVANCE, linkedIN

Targeting telomerase with an HLA class II-restricted TCR for cancer immunotherapy.

Freshly published in Molecular Therapy

and Combinatorial CAR design improves target restriction.

Published in Journal of Biological Chemistry

Your are most welcom to read the first article here and the second here !

Inven2 celebrates 10 years of collaboration with leading senior researchers bringin new products and services on market. Therefore, find out more informations about Dr. Else-Marit Inderberg's (group leader and head of immunomonitoring) interview here !

Else Marit Inderberg på laben. Foto: Elisabeth Kirkeng Andersen.

The Seahorse XFe96 Analyzer (Agilent) measures the oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of live cells in a 96-well plate format.

OCR and ECAR rates are key indicators of mitochondrial respiration and glycolysis as well as ATP production rate. Together these measurements will provide a clear view of cellular metabolic function in cultured cells such as Chimeric Antigen Receptor (CAR)- and T-cell Receptor (TCR)-modified cells !

More informations here !

Credit: Janne Nestvold (OCCI)

Solveig Mjelstad Olafsrud (Engineer) and Benjamin Caulier (Postdoc) will take care of it !

Immunotherapy & Biotechnology: Validation of humanized Chimeric Antigen Receptors targeted against solid tumour

Find out more here !

Any questions about the position can be sent to the following e-mail address: sebastw@rr-research.no

Join the Oslo Cancer Cluster virtual event presented during Oslo Innovation Week (September 2020) and hear from stakeholders who inspire innovation, protect innovation and provide the necessary tools for innovation.

Found out more at OCCi website !

Credit: OCCi

This is how lab discussions emerge ;)

Here is Patrik Vernhoff (Postdoc NFR, our favorite biostatistician !) air-presenting vanishing expression profil on PCA.

Credits: SW

Don't miss the journal club of Monday, 14th of September 2020.

Benjamin CAULIER, Postdoc, will present an article published in Immunity that deals with sustainability of CAR expression and response persistence using Ubiquitination modifications.

Credits: From Wentao Li et al:, Article in Immunity Volume 53, ISSUE 2, P456-470.e6, August 18, 2020

The journal club will take place in the usual 5^th floor meeting room after the lab meeting (around 12h). See you there !

Hakan Köksal will present: Designed protein logic to target cells with precise combinations of surface antigens (Marc J. Lajoie et al., 2020) (https://science.sciencemag.org/content/early/2020/08/19/science.aba6527.full)

Nadia Mensali will present: An RNA vaccine drives immunity in checkpoint-inhibitor-treated melanoma ( Ugur Sahin et al., 2020) (https://www.nature.com/articles/s41586-020-2537-9)

The Zoom link will be uptaded soon, make sure to schedule this event in your agenda !

The Norwegian biotech Zelluna Immunotherapy, based on a licence from our Translational Research Unit (Group leaders: Else Marit Inderberg and Sebastien Wälchli's group), received NOK 83 million to pursue their development on TCR-redirected NK cells !

We are very happy that an innovation developped five years ago by the early team (G. Gaudernack, G. Kvalheim, EM Inderberg and S. Wälchli) is now being developed as a therapeutic product. We thank our co-workers for their hard work, Inven2 for their support in the commercial development, and NFR, Helse Sør-Øst and Kreftforeningen  for their financial support and guidance.  

The team in Boston concluded that lobster is better than business:

The concept (with the old name):

Design: P. Dillard

Find more about this here !

We published a new review in Scandinavian Journal of Immunology on how to adapt TCR redirected T-cells therapy to fight melanoma!

Make sure to have a look here!

We published a review on putative targets in ovarian cancer for CAR therapy in Scandinavian Journal of Immunology! Make sure to have a look here!

We published a new article in BMC Biotechnology on how to use colorectal cyst as a tool for the pre-clinical development of CAR T-cells.

Make sure to check the article here!

Hakan Köksal successfully defended his Ph.D. entitled “Novel designs and approaches in CAR T cells” Thursday 28th May.

All of his colleagues are proud of his work and his achievements!

M.Sc Hakan Köksal at Institute of Clinical Medicine will be defending the thesis “Novel designs and approaches in CAR T cells” for the degree of PhD (Philosophiae Doctor).

Due to the current pandemic situation, the University of Oslo is closed and the public defence will be held as a video conference over Zoom here.

The defence will follow regular procedure as far as possible, hence it will be open to the public and the audience can ask ex auditorio questions when invited to do so.

Else Marit Inderberg and Nadia Mensali took part in a paper showing the treatment of posttransplantation lymphoproliferative disease by using retransplantation, make sure to check it here!

Baoyan Bai, June Helen Myklebust and Sébastien Wälchli just published a protocol on how to edit B cell lymphoma using CRISPR/Cas9 technology, make sure to have a look here!

Sébastien Wälchli and Mouldy Sioud published also a protocol on CRISPR/Cas9 as a tool for adoptive cell therapy. More information here!

Finally, Nicholas Casey published recently a paper on the use of RNAi with TCR transfer to enhance safety and efficiency. Follow the guide!

We participated to the EATRIS annual meeting in Lisbon and presented our work. We thank all the team for their great work and their commitment in this infrastructure.

Sympathetic improvement of cancer vaccine efficacy

Full text can be found here

A recent article in Nature Immunology showed a new type of T-cells that could improve cell-based therapies.

Forskning has asked Else Marit her opinion about it and about the latest developments in this field. Make sure to have a look here!

A new review from Else Marit Inderberg and Sebastien Wälchli was published in Cancer Immunology, Immunotherapy. The review described how we can isolate and identify therapeutic TCRs from long-term surviving patients.

Make sure to have a look here!

Amanda Ruud defended her Master at the University of Oslo, Wednesday (18/12). Her thesis title was: "Identification and pre-clinical assessment of patient-derived TCRs for solid tumor immunotherapy"

Pierre won the award with his talk entitled: "Targeting telomerase with an MHC class II restricted TCR for cancer immunotherapy". Make sure to have a look at his abstract!

We will be at the annual NSI meeting, the 29th of November!

We will talk about our latest progress on CAR and TCR therapy. See you there!

Sebastien Wälchli was invited at the 1st meeting ASEICA-ASPIC on Immunotherapy to talk about TCR based ACT.

Sebastien Walchli, a group leader in our department, gave an interview to the technical transfer office of the hospital: Inven2.

Make sure to check it here !

Emmanuelle Benard will present our last improvements on CD37 CAR T-cell therapy. Make sure to check the program of the conference and her abstract !

Nadia Mensali and Marit R. Myhre published a new study entitled: "Preclinical assessment of transiently TCR redirected T cells for solid tumour immunotherapy". A copy of the study published in Cancer Immunology, Immunotherapy can be found here.

We were at the last PIVAC (Progress in Vaccination Against Cancer) 2019 !

Else Marit and Hedvig presented our most recent developments !

Make sure to check their abstracts !

Yesterday Lizet Baken successfully defended her Bachelor Thesis at the Avans University of Applied Sciences.

Part of this thesis was performed in our lab where she has been working on an anti-Ig-lambda CAR.

Congratulations Lizet, we are all very proud of you!

We published a new paper on the CAR manufacturing from hybridoma to T-cell expression. The paper is published in Antibody Therapeutics, make sure to have a look here and here !

Else Marit Inderberg, Sebastien Wälchli, Nadia Mensali and Elizabeth Baken will attend CIMT 2019 meeting in Mainz (21 to 23 May).

Make sure to look at their abstracts.

CIMT website

CIMT program

We published pre-clinical results of the first 100% Norwegian CAR: CD37CAR, an alternative to CD19CAR.

Make sure to have a look here and here !

Our collaborators published a new paper on TIGIT and PD-1 as markers of reduced effector function of T-cells in B-cell lymphoma.

Make sure to have a look here !

Gunnar Kvalheim, Hakan Köksal and Pierre Dillard will be at the next AACR meeting (29th of March to 3rd of April, Atalanta) to talk about new developments in CAR.

Hakan will present a novel combination method for IGKCAR that prevents B-cell aplasia.

Pierre will present the pre-clinical validation of CD37CAR, an alternative to CD19CAR.

Make sure to have a look!

Pierre Dillard will present our rescent work on the pre-clinical validation of CD37 CAR T-cells in Barcelona (11 - 13 March 2019).

Make sure to take a look at the abstract and at the conference website !

We published a new paper on redirected NK cells, transduced with TCR.

Make sure to take a look at the paper here !

New publication from Nadia Mensali in OncoImmunology.

This article decribe the loading of MCHI and II molecules by using CD74 constructs.

Make sure to check it out here!

We published a new review on the treatment of osteosarcoma by CAR therapy.

Make sure to have a looke here !

We published a new protocol on the assessment of CAR activity by spheroids. Have a look at our paper here and on the OCCi website news.

Emmanuelle Benard who did her PhD in Biophysics and Immunology in Marseille, just joined us ! 

She will be in charge of the development of new CAR T-cells.

We had our first JOVE shooting on a new metod to generate spheroid for CAR T-cells screening.

Make sure to give it a look (once it is out) !

PIVAC 2018 was held in Oslo, co-organized by the department of Cell Therpay. The conference permitted to share new advance in the field of vaccination and future prospects for immunotherapy. 

Gunnar Kvalheim, Else Marit Inderberg, Sebastien Wälchli and Nadia Mensali will attend CIMT 2018 meeting in Mainz (15 to 17 May).

Make sure to look at their abstracts.

CIMT website

CIMT program

Sebastien Wälchli will present the depatment of Cellular Therapy at the 25th Porto Cancer Meeeting.

More details here and here! 

Pierre Dillard will attend the 2018 meeting of the American Association for Cncer Research (AACR). He will present our recent work on universal killer, make sure to have a look !

Human c-SRC kinase (CSK) overexpression makes T cells dummy

Patient safety upon immunotherapy treatment is a challenge that still need to be adressed.

In this publication we report how by affecting c-SRC kinase we can make T-cells "dummy": effector cells are still able to recognize and bind to the target but unable to carry on with their activation. This mechanism could potentially open the way for a safe-lock system permitting to reduce off-target toxicity.

Image from: Ellen Tenstad/Science Shaped

Nadia Mensali was awarded with the presentation prize for her talk on the Universal Killer! See below to learn more

Nadia Mensali and Pierre Dillard will attend the 2017 meeting of the Society for ImmunoTherapy of Cancer (SITC). They will present our recent work on universal killer and CD4 Adoptive cell therapy, make sure to have a look !

Our team published a new paper about using TCR in CAR design, make sure to have a look here !

The new Oslo Cancer Cluster presentation book is out!

Have a look here: 

OCC presentation

 Marit Renee Myhre has been selected to give a talk at  the 17th International Conference on Progress in  Vaccination Against Cancer (PIVAC 2017) in Loutraki,  Corinth, Greece

 She will present our latest work on our new CD4-based therapy, make sure to have a look at the abstract if you want  to learn more !

Our team has published a new paper in Translational Cancer Research:

New publication in our team: therapeutic T-cell receptors in healthy donor blood, is safety predictable? by Hakan Köksal and Sébastien Wälchli.
They are commenting a paper published in 2017 in Blood by Jahn et al. that presents a novel TCR-based therapy trageting intracellular transcription factor. Make sure to have a look at both papers!