Optimal voltages and Signal Spread Matrix Aria II

The Core facility staff would be happy to help you plan and execute multi-parameter flow experiments, however here are some key features that may help you do it yourself.

Choose a color that recieves little spread for markers that you are investigating double positives or weak positive

Choose a color that donates little spread for your "backbone markers", or else they will impact your entire panel

Avoid bad spillover spread combinations in your panel, but if you have to use those colors use them on mutually exclusive markers (e.g. CD3 vs CD19)

 
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