FACSMelody cell sorter(Gaustad) with 3 lasers 488/640/405
This user operated cell sorter is based on the same sorting technology as FACSAria.
The BD FACSMelody™ Cell Sorter with a 4-way cell sorting plus plate sorting makes cell sorting accessible to more researchers. This user operated cell sorter is based on the same sorting technology as FCASAria. The automation and simplified software, BD FACSChorus™, eliminates manual steps of setup, and save the scientist’s time with simplified operation.
Comparing to setting up FacsAria cell sorter, this FACSChorus™ Software reduce 55% of steps of starting up, QC, calibrating Dropdelay.
There are 3 lasers on this machine, 488nm/405nm/640 nm, with the availability of max 9 colors combinations.
There is only one nozzle size of 100 µm using on FacsMelody, which most of researchers prefer for their experiments.
The fixed alignment means there is no lengthy procedure with alignment beads required at startup.
Only perform instrument QC when needed. It is not required each time the sorting nozzle is installed. Even with instrument QC, you can typically run your sample in less than 17 minutes compared to less than 8 minutes without QC.
Automated stream setup and steering into tubes or plates saves valuable minutes.
Examples of the features:
- Automated setup of laser delay, drop delay and side streams
- An interface which leads you through workflows
- On-screen instructions and tool tips
- Index sorting acquisition and analysis
- Default plots and sort gates
- Automatic compensation updates when voltages are changed
- Prevention of tube overflow
- Deflection plates turned on automatically, only when necessary, to guard operator safety
- FCS 3.1 compatible
- Proven BD FACS TM Accudrop and BD Sweetspot technology
Guick user guide:
https://static.bdbiosciences.com/documents/BD-FACSMelody-FACSChorus-Quick-Reference-Guide.pdf
Complete user guide from BD:
https://bcf.technion.ac.il/wp-content/uploads/2018/05/FACSMelody-Users-Guide.pdf
For free online practical training: https://www.bdbiosciences.com/en-us/support/training/e-learning-courses
More details can be found here https://www.bdbiosciences.com/en-us/instruments/research-instruments/research-cell-sorters/facsmelody
Instrument location is at room A3.M047.
Access for Using FACSMelody for DIY Sorting
- User Training: A trained user may use the Melody Cell Sorter without supervision.
- Fees for ''Onsite Practical Training'': 1750kr/h/person
- OUS/UiO users: 350 kr/h (DIY) (payment via UiO account requires mva)
- Academic users outside HSØ region: 525 kr/h (DIY) (ex. mva)
- Commercial users collaborator : 700 kr/h (DIY ex. mva)
- Commercial users: 1400 kr7h (DIY ex mva)
- Sorting service: 700 kr/h (payment via UiO account requires mva)
- Sorting assisted by the staff is available for less than 3 times experiments from the same user including the same research group.
Free online training: https://www.bdbiosciences.com/en-us/support/training/e-learning-courses