3-Laser Aurora Cell sorter (Montebello)

A 3-Laser full-spectrum cell sorter, with three scattering channels, and 38 fluorescence channels. The state-of-the-art optics and low-noise electronics provide excellent sensitivity and resolution. Flat-top laser beam profiles, combined with a uniquely designed fluidics system, translate to outstanding performance at high sample flow rates.

The Cytek Aurora’s use of full spectrum flow cytometry combined with the SpectroFlo® software’s real-time unmixing capability provides greater fluorochrome choice and panel flexibility and allows users to quickly visualize data and statistics. All this results in the ultimate flow cytometry solution for deep immunoprofiling of > 20 colors.

 

Room: K03.078

Specifications:

  • Can sort up to 25,000 events/sec, within a <65-gate deep gating hierarchy.
  • Sort up to 6 different populations simultaneously with 1.5 mL tubes, up to 4 with 5 mL tubes.
  • Sort into 96 well plates, with optional index sorting.
  • Aerosol containment.
  • Temperature control on samples and collected tubes from 4° to 37°C.
  • Flat-Top laser beam profile with narrow vertical beam height optimized for small particle detection.
    • 405 nm: 100 mW, 488 nm: 50 mW, 640 nm: 80 mW
  • Full-spectrum analysis of 38 fluorescence channels using proprietary high sensitivity Coarse Wavelength Division Multiplexing (CWDM) semiconductor array per laser:
    • 405 nm: 16 channels unevenly spaced bandwidth from 420-829 nm
    • 488 nm: 14 channels unevenly spaced bandwidth from 498-829 nm.
    • 640 nm: 8 channels unevenly spaced bandwidth from 652-829 nm.
  • 3 scatter parameters: FSC and SSC-B (both from 488 nm laser), in addition to SSC from 405 nm laser (better for submicron particles)
  • PC-based SpectroFlo® software

Access:

  • Full Service Cell Sorting: available by appointment with the Core Facility.
  • Instrument start-up by staff: DIY sorting after start-up (requires pre-training), and one hour Assistance fee will be charged on top of the booking time.
  • Super User Training: A trained super-user per research group may use the Aurora Cell Sorter without supervision. 

For appointments please e-mail us at icr-flow-cytometry@ous-research.no

Booking for trained users at icr-booking.no.

 

Sorting fee; Full-service/DIY:

  • Academic user within Helse Sør-Øst region: 700/350 kr/h (ex. mva, non-HSØ account requires mva)
  • Academic user outside Helse Sør-Øst: 1050/525 kr/h (ex. mva)
  • Commercial user: 2800/1400 kr/h (ex. mva)

Assistance fee: 500 kr/h

To find out what colors to use and it's peak channel visit: spectrum.cytekbio.com

AURORA STANDARD OPTICAL CONFIGURATION:

  • 3 scatter parameters: FSC and SSC-B (both from 488 nm laser), in addition to SSC from 405 nm laser (better for submicron particles)
  • PC-based SpectroFlo® software.
 
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