About the core facility

The Flow Cytometry Core Facility has provided flow cytometry analysis and cell sorting services to interested users for 25 years.

The FCCF provides sorting of cells, bacteria, chromosomes, beads and other micro organisms based on light scatter and/or fluorescent markers. Cells can be sorted under sterile conditions and with high purity enabling the recovered cells to be cultured. It is possible to sort cells according to antigen/flourescent proitein expression, DNA content or cell function, or a combination thereof.

Cells can also be sorted to retrieve sub-populations for RNA and protein extraction or for morphological analysis. Sorted cells can be collected into tubes (one to four tubes/populations) or deposited as single cells into multiwell plates or onto slides. This can be useful for cloning transfected cells, hybridomas, PCR, FISH, etc. The sorting can be optimized according to whether purity, recovery or count accuracy is most important.

We also provide training and assistance for flow cytometry analysis of samples as well as technical expertise and advice for experimental planning and design, cell preparation, analysis and data handling.

Funding

The flow cytometry core facility is a non-profit facility and is supported by grants from Helse Sør-Øst, OUS and UiO, as well as nominal user fees.

Users

Services are provided primarily for academic users from the Helse Sør-Øst region. In addition, other interested national and international academic users as well as commercial actors are encouraged to contact FCCF management/staff to contract services.

Fees

Trained users will be able to use our instruments (DIY) for a fee per hour to cover running expenses (fluorescent beads, tubing, instrument service and sheath fluid).Sorting or analysis assistance performed by Core facility staff will be charged per hour. Detailed price information can be found on the page for each instrument.

Location

The Flow Cytometry Core Facility at OUS divides its instrument parks between Montebello, Gaustad and Ullevål. 

Montebello:
Room K03.078(East wing) / K03.080(East wing) / K03.020(West wing), Department of Radiation Biology, Institute for Cancer Research,
The Norwegian Radium Hospital, Montebello, 0310 Oslo

Gaustad: 
Room A3.M037/ A3. M047, Institute of Pathology, Rikshospitalet, Sognsvannsveien 20, 0027 Oslo

Ullevål:
Room 0606030, Midtblokka 6th floor
Department for Medical Biochemistry, Ullevål hospital,
Kirkeveien 166, 0450 Oslo

 
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