About the core facility

Since 1995, the Flow Cytometry Core Facility (FCCF) has offered comprehensive flow cytometry analysis and cell sorting services. Our services include:

Cell Sorting: We sort cells, organelles, fixed bacteria, and other microorganisms using light scatter and fluorescent markers. Our semi-sterile sorting conditions ensure high purity, enabling the recovered cells to be cultured. We can sort cells by antigen/fluorescent protein expression, DNA content, cell function, or a combination thereof. This is useful for retrieving clones/sub-populations for further culture, DNA/RNA/protein extraction, or morphological analysis. Cells can be collected in tubes (one to six tubes/populations) or deposited as single cells into multiwell plates or onto slides.

Analyzer Training and Assistance: We offer training and assistance for both conventional and full-spectrum fluorescence, as well as mass flow cytometry analysis. We provide technical expertise and advice for experimental planning and design, cell preparation, analysis, and data handling.

Instruments

Our latest additions include:

  • ID7000 full-spectrum instruments at Gaustad/Rikshospitalet

  • 5-laser Aurora analyzer and 3-laser Aurora Cell sorter at Radium/Montebello

  • FACSMelody Cell sorter at Ullevål

  • Within the last 5 years, we acquired a mass cytometry imaging unit (Hyperion at Radium, up to 40 parameters), the analyzer FACSymphony A5 (Radium), and the FACSMelody Cell sorter at Gaustad.

Funding

The FCCF operates as a non-profit facility, supported by grants from Helse Sør-Øst, OUS, UiO/NFR, as well as user fees. Funding for new instruments has also been provided by several UiO centers of excellence, Norsk Hydros Fond, and Radiumhospitalets Legater.

Users

Our services are primarily offered to academic users from the Helse Sør-Øst region. National and international academic users, as well as commercial entities, are welcome to contact FCCF management/staff to contract services.

Fees

Trained users can utilize our instruments on a DIY basis for an hourly fee covering running expenses (reagents, materials, and service). Sorting or analysis assistance provided by Core Facility staff is also charged per hour. Detailed pricing information is available on the page for each instrument.

Location

The Flow Cytometry Core Facility at OUS houses its instruments across three locations: Montebello, Gaustad, and Ullevål.

Montebello:
All rooms in K-building ("Forskningsbygget"): K03.078 and K03.080 (both East wing, "Strålingsbiologi") / K03.020 (West wing, "Kreftimmunologi"), Department of Core Facilities, Institute for Cancer Research,
The Norwegian Radium Hospital, Montebello, 0310 Oslo

Gaustad: 
Room A3.M037/ A3. M047/A3.M036A, Institute of Pathology, Rikshospitalet, Sognsvannsveien 20, 0027 Oslo

Ullevål:
Room 0606030, Midtblokka 6th floor
Department for Medical Biochemistry, Ullevål hospital,
Kirkeveien 166, 0450 Oslo

 
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