5-Laser Aurora Analyzer (Montebello)

A 5-Laser full-spectrum analyzer, with three scattering channels, and 64 fluorescence channels. The state-of-the-art optics and low-noise electronics provide excellent sensitivity and resolution. Flat-top laser beam profiles, combined with a uniquely designed fluidics system, translate to outstanding performance at high sample flow rates.

The Cytek Aurora’s use of full spectrum flow cytometry combined with the SpectroFlo® software’s real-time unmixing capability provides greater fluorochrome choice and panel flexibility and allows users to quickly visualize data and statistics. All this results in the ultimate flow cytometry solution for deep immunoprofiling of > 40 colors.

Room: K03.078

Specifications:

  • 5 Flat-Top laser beam profile with narrow vertical beam height optimized for small particle detection.
    • 405 nm: 100 mW, 488 nm: 50 mW, 640 nm: 80 mW, 355 nm: 20 mW, 561 nm: 50 mW
  • Full-spectrum analysis of 64 fluorescence channels using proprietary high sensitivity Coarse Wavelength Division Multiplexing (CWDM) semiconductor array per laser:
    • 405 nm: 16 channels unevenly spaced bandwidth from 420-829 nm.
    • 488 nm: 14 channels unevenly spaced bandwidth from 498-829 nm.
    • 640 nm: 8 channels unevenly spaced bandwidth from 652-829 nm.
    • 355 nm: 16 channels unevenly spaced bandwidth from 365-829 nm.
    • 561 nm: 10 channels unevenly spaced bandwidth from 567-829 nm.
  • 3 scatter parameters: FSC and SSC-B (both from 488 nm laser), in addition to SSC from 405 nm laser (better for submicron particles)
  • PC-based SpectroFlo software.

Access:

Trained users may use the Aurora without Core Facility supervision. 

Training will be set up for interested users after appointment.

Please e-mail us at icr-flow-cytometry@ous-research.no

Booking for trained users at icr-booking.no.

 

Analysis fee (DIY):

  • Academic user within Helse Sør-Øst region: 250 kr/h (ex. mva, non-HSØ account requires mva)
  • Academic user outside Helse Sør-Øst: 375 kr/h (ex. mva)
  • Commercial user: 1000 kr/h (ex. mva)

Fee assistance: 500 kr/h + instrument fee

To find out what colors to use and it's peak channel visit: spectrum.cytekbio.com

AURORA STANDARD OPTICAL CONFIGURATION:

 
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