❭ Research by medical division ❭ Cancer Medicine ❭ Institute for Cancer Research ❭ Dept. of Molecular Cell Biology ❭ Jorrit Enserink's group ❭ Protocols ❭

alpha-factor synchronization (arrest-release)

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TCA Precipitation for S. cerevisiae

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Random sporulation

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Mini prep

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Induction test of a recombinant protein

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His-FLAG-SUMO targets pull down in denaturing conditions

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GST-fusion protein purification

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Flow Cytometry in yeast (Sytox green)

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Flow cytometry in yeast (Propidium Iodide)

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Coomassie Staining and Destaining

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Immunoprecipitation

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Preparing yeast protein extracts for IP

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ChIP-MS

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ChIP in Yeast (for ~ 20 ChIPs)

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Analysis of RNA capping in vivo (RNA IP)

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BaF3 cell transfection protocol

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CellTiter-Glo Assay

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Protein isolation using TCA precipitation

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Recent publications

Corrales J, Ramos-Alonso L, González-Sabín J, Ríos-Lombardía N, Trevijano-Contador N, Engen Berg H, Skottvoll FS, Moris F, Zaragoza O, Chymkowitch P, Garcia I, Enserink JM (2025)
Correction for Corrales et al., "Characterization of a selective, iron-chelating antifungal compound that disrupts fungal metabolism and synergizes with fluconazole"
Microbiol Spectr, e0073025 (in press)
DOI 10.1128/spectrum.00730-25, PubMed 41117562

Andresen S, Al Outa A, Formica M, Enserink J, Knævelsrud H (2025)
Improved detection of lipidated Atg8a by immunoblotting in Drosophila melanogaster cells and tissues enables precise investigation of Atg8a flux and its termination
Autophagy, 21 (11), 2497-2517
DOI 10.1080/15548627.2025.2508551, PubMed 40426043

Fuentes-Martín R, Ayuda-Durán P, Hanes R, Gallego-Yerga L, Wolterinck L, Enserink JM, Álvarez R, Peláez R (2024)
Promising anti-proliferative indolic benzenesulfonamides alter mechanisms with sulfonamide nitrogen substituents
Eur J Med Chem, 275, 116617
DOI 10.1016/j.ejmech.2024.116617, PubMed 38959729



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Get in touch

Department of Molecular Cell Biology, Institute for Cancer Research
The Norwegian Radium Hospital, Montebello, N-0379 Oslo, Norway
Telephone +47 22 78 19 82 (Enserink), +47 22 78 12 69 (Dept. Secretary)
Email: j.m.enserink@ibv.uio.no

Oslo University Hospital is a part of Southern and Eastern Norway Regional Health Authority.

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