❭ Research by medical division ❭ Cancer Medicine ❭ Institute for Cancer Research ❭ Dept. of Molecular Cell Biology ❭ Jorrit Enserink's group ❭ Protocols ❭
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alpha-factor synchronization (arrest-release)

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TCA Precipitation for S. cerevisiae

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Random sporulation

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Mini prep

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Induction test of a recombinant protein

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His-FLAG-SUMO targets pull down in denaturing conditions

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GST-fusion protein purification

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Flow Cytometry in yeast (Sytox green)

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Flow cytometry in yeast (Propidium Iodide)

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Coomassie Staining and Destaining

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Immunoprecipitation

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Preparing yeast protein extracts for IP

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ChIP-MS

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ChIP in Yeast (for ~ 20 ChIPs)

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Analysis of RNA capping in vivo (RNA IP)

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BaF3 cell transfection protocol

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CellTiter-Glo Assay

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Protein isolation using TCA precipitation

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Recent publications

Enserink JM (2022)
The SUMO stress response in transcriptional regulation: Causal relationships or secondary bystander effects?
Bioessays, 44 (7), e2200065
DOI 10.1002/bies.202200065, PubMed 35655365

Ramos-Alonso L, Garcia I, Enserink JM, Chymkowitch P (2022)
Analysis of the pheromone signaling pathway by RT-qPCR in the budding yeast Saccharomyces cerevisiae
STAR Protoc, 3 (1), 101210
DOI 10.1016/j.xpro.2022.101210, PubMed 35265859

Enserink JM, Chymkowitch P (2022)
Cell Cycle-Dependent Transcription: The Cyclin Dependent Kinase Cdk1 Is a Direct Regulator of Basal Transcription Machineries
Int J Mol Sci, 23 (3)
DOI 10.3390/ijms23031293, PubMed 35163213



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Get in touch

Department of Molecular Cell Biology, Institute for Cancer Research
The Norwegian Radium Hospital, Montebello, N-0379 Oslo, Norway
Telephone +47 22 78 19 82 (Enserink), +47 22 78 12 69 (Dept. Secretary)
Email: jorrit.enserink@rr-research.no

Oslo University Hospital is a part of Southern and Eastern Norway Regional Health Authority.

Webmaster for research pages: Trond Olav Berg

Email: trond.olav.berg@rr-research.no

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