To assess the functional and causal effects of the identified epigenetic alterations, we are using CRISPR epigenetic editing. In breast cancer cell lines with stable and inducible expression of deactivated Cas9 fused with the catalytic domain of DNMT3A, we can induce DNA methylation at specific loci. The effects of induced DNA methylation is assessed by measuring transcription factor occupancy, target gene expression, and cell proliferation and migration.
Multi-modal meta-analysis of cancer cell line omics profiles identifies ECHDC1 as a novel breast tumor suppressor
Mol Syst Biol, 17 (3), e9526
Epigenome-wide gene-age interaction analysis reveals reversed effects of PRODH DNA methylation on survival between young and elderly early-stage NSCLC patients
Aging (Albany NY), 12 (11), 10642-10662
Independent Validation of Early-Stage Non-Small Cell Lung Cancer Prognostic Scores Incorporating Epigenetic and Transcriptional Biomarkers With Gene-Gene Interactions and Main Effects
Chest, 158 (2), 808-819