CRISPR epigenetic editing

To assess the functional and causal effects of the identified epigenetic alterations, we are using CRISPR epigenetic editing. In breast cancer cell lines with stable and inducible expression of deactivated Cas9 fused with the catalytic domain of DNMT3A, we can induce DNA methylation at specific loci. The effects of induced DNA methylation is assessed by measuring transcription factor occupancy, target gene expression, and cell proliferation and migration. 



Marie Engkvist Rosvold, Jørgen Ankill, Thomas Fleischer

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