Attune Nxt Configurations

Excitation laser

Emission filter (nm)

Channel

Antibody labels

Compatible fluorescent proteins

Violet–405 nm

440/50

VL1

Alexa Fluor™ 405
Pacific Blue™

Sirius
Azurite
eCFP
eBFP
mTarquoise
Cerulean

512/25

VL2 

Pacific Green™

vGFP

603/48

VL3

Pacific Orange™
Qdot™ 605

710/50

VL4

Qdot™ 705

Blue–488 nm

530/30

BL1

Alexa Fluor™ 488
FITC

eGFP
Emerald
eYFP

590/40

BL2

PE
PE-Alexa Fluor™ 610
PE-Texas Red™ 
Qdot™ 585

695/40

BL3

PE-Alexa Fluor™ 700
Tri-Color™
PE-Cy®5.5
PerCP
PerCP-Cy®5.5
Qdot™ 705 

Yellow–561 nm

585/16

YL1

PE

 mOrange
RFP
dTomato

620/15

YL2

PE- Alexa Fluor 610
PE-Texas Red™

mCherry
DsRed
mKate
mStrawberry

695/40

YL3

PE-Alexa Fluor™ 700
PE-Cy®5.5
Qdot™ 705
Tri-Color™

780/60

YL4

PE-Cy®7
Qdot™ 800

Red–637 nm

670/14

RL1

APC
Alexa Fluor® 647
Qdot™ 655

720/30

RL2

Alexa Fluor™ 680
Alexa Fluor™ 700
APC-Alexa Fluor™ 700
Qdot™ 705

780/60

RL3

APC-Alexa Fluor™ 750
APC-Cy® 7
Qdot™ 800