Fortessa (Gaustad)
The BD LSRFortessa™ cell analyzer has 4 lasers that can detect up to 18 colors simultaneously. Blue, Green, red and violet—which provides a flexibility in laser wavelengths so assay design can be optimized using the latest fluorescent dyes and substrates.
The optics system consists of laser excitation optics that illuminate cells in the sample, and collection optics that direct light scatter and fluorescence signals through spectral filters to detectors. Innovative designs for both the excitation optics and collection optics in BD LSRFortessa systems reduce excitation losses and optimize collection efficiency for increased sensitivity and resolution.
About the detectors, there are three octagons for the blue, violet and yellow/green lasers, and a trigon for the red laser.
Access:
Trained users may use the Fortessa without Core Facility supervision.
Location is at room A3.M047.
Training course:
Training can be set up for interested users after appointment. For all training, choose the ''Practical Training'' in the booking schedule.
Fees for using analysis flowcytometers
Analysis (DIY):
- OUS/UiO users: 175 kr/h (ex. mva, payment via UiO account requires mva)
- Academic users outside HSØ region: 260 kr/h (ex. mva)
- Commercial users collabrator: 350 kr/h
- Commercial users : 700 kr/h
- Assisted sample acquisition: +500 kr/h
Practical Training: 1750 kr/h/person
The configuration map can be found under the Instrument top menu bar.
Optics & Fluorochromes for Fortessa
|
Laser (Excitation) |
PMT (FL) |
BP Filter (Emission) |
LP |
Fluorochromes |
|
|
Blue laser 488 nm |
A |
|
710/50 |
685 |
Percp-Cy5.5, eFluro 710, Pe-Cy5 |
|
B |
|
530/30 |
505 |
FITC, Alexa 488, GFP,SYTOXGreen,YFP |
|
|
C |
SSC |
488/10 |
SSC |
|
|
|
D |
|
|
|
|
|
|
E |
|
|
|
|
|
|
F |
|
|
|
|
|
|
G |
|
|
|
|
|
|
H |
|
|
|
|
|
|
Yellow/ Green Laser 561 nm |
A |
|
780/60 |
750 |
Pe-Cy7 |
|
B |
|
710/50 |
685 |
Percp-Cy5.5, eFluro 710, Pe-Cy5.5 |
|
|
C |
|
670/30 |
635 |
Pe-Cy5 |
|
|
D |
|
610/20 |
600 |
PI, Texas-Red, mCherry |
|
|
E |
|
585/15 |
|
PE, DsRed |
|
|
F |
|
|
|
|
|
|
G |
|
|
|
|
|
|
H |
|
|
|
|
|
|
Violet Laser 405 nm |
A |
|
710/50 |
690 |
BV 711 |
|
B |
|
660/20 |
630 |
BV 650 |
|
|
C |
|
610/20 |
595 |
BV 605 |
|
|
D |
|
540/30 |
535 |
Qdot 565, Pacific Orange, V540 |
|
|
E |
|
525/50 |
505 |
Alexa 430, Cascade Yellow, AmCyan,V 500 , DAPI |
|
|
F |
|
450/50 |
|
Pacific Blue, Hoechst, Alexa 405, V450, Cascade Blue, BD Horizon |
|
|
G |
|
|
|
|
|
|
|
H |
|
|
|
|
|
Red Laser 640 |
A |
|
780/60 |
750 |
APC-Cy7, APC-H7, Alexa 750 |
|
B |
|
730/45 |
690 |
Alexa 680, Alexa 700 |
|
|
C |
|
670/14 |
|
APC, Cy5, Alexa 647, Alexa 633, CYTOX Red |
|