
Sébastien Wälchli
- Principal Investigator; PhD
- +47 22 78 13 16
Group leader
Education:
|
2016 |
Present |
Senior scientist (group leader-OUS leader training 2019) |
Section for Cellular Therapy, Radiumhospitalet, Oslo |
|
2013 |
2016 |
Senior scientist (project leader) |
Sections for Cancer Immunology and Cellular Therapy, Radiumhospitalet, Oslo |
|
2007 |
2013 |
Scientist |
Dept of Immunology, IKF, Radiumhospitalet, Oslo |
|
2003 |
2007 |
Postdoc |
Dept of Biochemistry, IKF, Radiumhospitalet, Oslo |
|
2002 |
2003 |
Visiting scientist |
Medical school of Geneva, Geneva, Switzerland |
|
1998 |
2002 |
PhD student |
Serono Pharmaceutical Research Institute (Hooft), Geneva, Switzerland |
Research interests/projects:
|
SW co-leads the translational research lab at the section for Cellular therapy, OUS, a newly established pre-clinical entity directly connected to the immunomonitoring unit. SW leads the molecular biology of the T-cell receptor (TCR) and the Chimeric Antigen Receptor (CAR) development platforms. The TCR platform has led to the filling of numerous patents, the establishment of a biotech (Zelluna Immuntherapy AS, 2016) and the first TCR tested in human in Norway (ClinicalTrials.gov Identifier: NCT03431311). The CAR platform has completed the pre-clinical development of 2 candidates and is working on around 15 new constructs, most of them directed against solid tumours. Advanced universal therapeutic solutions are also in our pipeline (3 patents), and different innovative tools in the immunotherapeutic field (vaccination, effector cell improvement, etc.). More info here: www.linkedin.com/in/sebastw |
Awards
- Inven2 Innovation award 2023: "DogBiTE"
- OvaCure Award at the OvaCure Innovation Challenge 2020
- Inven2 Innovation award 2014: "A universal killer-T cell for personalized medicine"
- Top poster Award at CIMT (Association of Cancer Immunotherapy) in Mainz (Germany) 2012: "It takes two to tango: MHC-I and Invariant chain in harmony"
- Inven2 Innovation award 2011: "A novel method to produce soluble TcR"
Publications 2025
Pan-carcinoma sialyl-Tn-targeting expands CAR therapy to solid tumors
6 (9), 102350
DOI 10.1016/j.xcrm.2025.102350, PubMed 40925376
LILRB1-directed CAR-T cells for the treatment of hematological malignancies
39 (6), 1395-1411
DOI 10.1038/s41375-025-02580-z, PubMed 40186066
Publications 2024
CD20 expression regulates CD37 levels in B-cell lymphoma - implications for immunotherapies
13 (1), 2362454
DOI 10.1080/2162402X.2024.2362454, PubMed 38846084
Efficient CAR T cell targeting of the CA125 extracellular repeat domain of MUC16
12 (4)
DOI 10.1136/jitc-2023-008179, PubMed 38604812
CD37 is a safe chimeric antigen receptor target to treat acute myeloid leukemia
5 (6), 101572
DOI 10.1016/j.xcrm.2024.101572, PubMed 38754420
Transient TCR-based T cell therapy in a patient with advanced treatment-resistant MSI-high colorectal cancer
32 (6), 2021-2029
DOI 10.1016/j.ymthe.2024.04.009, PubMed 38582964
Protocol for production of tonic CAR T cells with dasatinib
6 (1), 103529
DOI 10.1016/j.xpro.2024.103529, PubMed 39739533
Protocol for the development of a bioluminescent AML-PDX mouse model for the evaluation of CAR T cell therapy
5 (4), 103522
DOI 10.1016/j.xpro.2024.103522, PubMed 39673705
Publications 2023
Efficient chimeric antigen receptor targeting of a central epitope of CD22
299 (7), 104883
DOI 10.1016/j.jbc.2023.104883, PubMed 37269947
Determination of CAR T cell metabolism in an optimized protocol
11, 1207576
DOI 10.3389/fbioe.2023.1207576, PubMed 37409169
In vitro re-challenge of CAR T cells
183, 335-353
DOI 10.1016/bs.mcb.2023.06.003, PubMed 38548418
ALPL-1 is a target for chimeric antigen receptor therapy in osteosarcoma
14 (1), 3375
DOI 10.1038/s41467-023-39097-x, PubMed 37291203
Publications 2022
CAR-Ts: new perspectives in cancer therapy
596 (4), 403-416
DOI 10.1002/1873-3468.14270, PubMed 34978080
PD-L1 CAR effector cells induce self-amplifying cytotoxic effects against target cells
10 (1)
DOI 10.1136/jitc-2021-002500, PubMed 35078921
How CAR T Cells Breathe
11 (9)
DOI 10.3390/cells11091454, PubMed 35563759
Mechanism of CD79A and CD79B Support for IgM+ B Cell Fitness through B Cell Receptor Surface Expression
209 (10), 2042-2053
DOI 10.4049/jimmunol.2200144, PubMed 36426942
A phase I/II escalation trial design T-RAD: Treatment of metastatic lung cancer with mRNA-engineered T cells expressing a T cell receptor targeting human telomerase reverse transcriptase (hTERT)
12, 1031232
DOI 10.3389/fonc.2022.1031232, PubMed 36439452
Publications 2021
Pharmacologic Control of CAR T Cells
22 (9)
DOI 10.3390/ijms22094320, PubMed 33919245
Targeting KRAS mutations with HLA class II-restricted TCRs for the treatment of solid tumors
10 (1), 1936757
DOI 10.1080/2162402X.2021.1936757, PubMed 34235003
In vivo experimental mouse model to test CD19CAR T cells generated with different methods
167, 149-161
DOI 10.1016/bs.mcb.2021.11.001, PubMed 35152992
Id2 Represses Aldosterone-Stimulated Cardiac T-Type Calcium Channels Expression
22 (7)
DOI 10.3390/ijms22073561, PubMed 33808082
"Built-in" PD-1 blocker to rescue NK-92 activity from PD-L1-mediated tumor escape mechanisms
35 (9), e21750
DOI 10.1096/fj.202100025R, PubMed 34424568
Rab7b regulates dendritic cell migration by linking lysosomes to the actomyosin cytoskeleton
134 (18)
DOI 10.1242/jcs.259221, PubMed 34494097
Publications 2020
Gene Editing in B-Lymphoma Cell Lines Using CRISPR/Cas9 Technology
2115, 445-454
DOI 10.1007/978-1-0716-0290-4_25, PubMed 32006416
SJI 2020 special issue: A catalogue of Ovarian Cancer targets for CAR therapy
92 (4), e12917
DOI 10.1111/sji.12917, PubMed 32557659
Targeting Telomerase with an HLA Class II-Restricted TCR for Cancer Immunotherapy
29 (3), 1199-1213
DOI 10.1016/j.ymthe.2020.11.019, PubMed 33212301
Colorectal cysts as a validating tool for CAR therapy
20 (1), 30
DOI 10.1186/s12896-020-00623-0, PubMed 32487146
Long-term surviving cancer patients as a source of therapeutic TCR
69 (5), 859-865
DOI 10.1007/s00262-019-02468-9, PubMed 31915853
Sympathetic improvement of cancer vaccine efficacy
16 (8), 1888-1890
DOI 10.1080/21645515.2019.1703456, PubMed 31977269
Combinatorial CAR design improves target restriction
296, 100116
DOI 10.1074/jbc.RA120.016234, PubMed 33234592
Correction to: Preclinical assessment of transiently TCR redirected T cells for solid tumour immunotherapy
69 (1), 159-161
DOI 10.1007/s00262-019-02409-6, PubMed 31776597
T cell receptor therapy against melanoma-Immunotherapy for the future?
92 (4), e12927
DOI 10.1111/sji.12927, PubMed 32640053
Next Generation of Adoptive T Cell Therapy Using CRISPR/Cas9 Technology: Universal or Boosted?
2115, 407-417
DOI 10.1007/978-1-0716-0290-4_22, PubMed 32006413
Publications 2019
TIGIT and PD-1 Mark Intratumoral T Cells with Reduced Effector Function in B-cell Non-Hodgkin Lymphoma
7 (3), 355-362
DOI 10.1158/2326-6066.CIR-18-0351, PubMed 30659053
Chimeric antigen receptor preparation from hybridoma to T-cell expression
2 (2), 56-63
DOI 10.1093/abt/tbz007, PubMed 33928223
Preclinical development of CD37CAR T-cell therapy for treatment of B-cell lymphoma
3 (8), 1230-1243
DOI 10.1182/bloodadvances.2018029678, PubMed 30979721
Treating osteosarcoma with CAR T cells
89 (3), e12741
DOI 10.1111/sji.12741, PubMed 30549299
NK cells specifically TCR-dressed to kill cancer cells
40, 106-117
DOI 10.1016/j.ebiom.2019.01.031, PubMed 30665853
Antigen-delivery through invariant chain (CD74) boosts CD8 and CD4 T cell immunity
8 (3), 1558663
DOI 10.1080/2162402X.2018.1558663, PubMed 30723591
Preclinical assessment of transiently TCR redirected T cells for solid tumour immunotherapy
68 (8), 1235-1243
DOI 10.1007/s00262-019-02356-2, PubMed 31214732
Simultaneous defeat of MCF7 and MDA-MB-231 resistances by a hypericin PDT-tamoxifen hybrid therapy
5, 13
DOI 10.1038/s41523-019-0108-8, PubMed 30993194
Publications 2018
A Spheroid Killing Assay by CAR T Cells
(142)
DOI 10.3791/58785, PubMed 30596389
Breadth and Dynamics of HLA-A2- and HLA-B7-Restricted CD8+ T Cell Responses against Nonstructural Viral Proteins in Acute Human Tick-Borne Encephalitis Virus Infection
2 (6), 172-184
DOI 10.4049/immunohorizons.1800029, PubMed 31022685
Artesunate shows potent anti-tumor activity in B-cell lymphoma
11 (1), 23
DOI 10.1186/s13045-018-0561-0, PubMed 29458389
Publications 2017
BMP-7 induces apoptosis in human germinal center B cells and is influenced by TGF-β receptor type I ALK5
12 (5), e0177188
DOI 10.1371/journal.pone.0177188, PubMed 28489883
Human c-SRC kinase (CSK) overexpression makes T cells dummy
67 (4), 525-536
DOI 10.1007/s00262-017-2105-9, PubMed 29248956
T cell therapy targeting a public neoantigen in microsatellite instable colon cancer reduces in vivo tumor growth
6 (4), e1302631
DOI 10.1080/2162402X.2017.1302631, PubMed 28507809
Fishing therapeutic T-cell receptors in healthy donor blood, is safety predictable?
Transl. Cancer Res., 6 3, S622-S624
DOI 10.21037/tcr.2017.05.25
A TCR-based Chimeric Antigen Receptor
7 (1), 10713
DOI 10.1038/s41598-017-11126-y, PubMed 28878363
Publications 2016
Enhancing Antitumor Immune Responses by Optimized Combinations of Cell-penetrating Peptide-based Vaccines and Adjuvants
24 (9), 1675-85
DOI 10.1038/mt.2016.134, PubMed 27377043
T-helper cell receptors from long-term survivors after telomerase cancer vaccination for use in adoptive cell therapy
5 (12), e1249090
DOI 10.1080/2162402X.2016.1249090, PubMed 28123886
Targeting B-cell neoplasia with T-cell receptors recognizing a CD20-derived peptide on patient-specific HLA
5 (5), e1138199
DOI 10.1080/2162402X.2016.1138199, PubMed 27467957
Distinct patterns of B-cell receptor signaling in non-Hodgkin lymphomas identified by single-cell profiling
129 (6), 759-770
DOI 10.1182/blood-2016-05-718494, PubMed 28011673
Publications 2015
Unpredicted phenotypes of two mutants of the TcR DMF5
425, 37-44
DOI 10.1016/j.jim.2015.06.006, PubMed 26079729
Deciphering the Nongenomic, Mitochondrial Toxicity of Tamoxifens As Determined by Cell Metabolism and Redox Activity
11 (1), 251-62
DOI 10.1021/acschembio.5b00734, PubMed 26569462
Soluble T-cell receptors produced in human cells for targeted delivery
10 (4), e0119559
DOI 10.1371/journal.pone.0119559, PubMed 25875651
Publications 2013
BiP negatively affects ricin transport
5 (5), 969-82
DOI 10.3390/toxins5050969, PubMed 23666197
Alloreactive cytotoxic T cells provide means to decipher the immunopeptidome and reveal a plethora of tumor-associated self-epitopes
111 (1), 403-8
DOI 10.1073/pnas.1306549111, PubMed 24344295
Invariant chain as a vehicle to load antigenic peptides on human MHC class I for cytotoxic T-cell activation
44 (3), 774-84
DOI 10.1002/eji.201343671, PubMed 24293164
Publications 2012
Role of Smad proteins in resistance to BMP-induced growth inhibition in B-cell lymphoma
7 (10), e46117
DOI 10.1371/journal.pone.0046117, PubMed 23049692
Nuclear import of exogenous FGF1 requires the ER-protein LRRC59 and the importins Kpnα1 and Kpnβ1
13 (5), 650-64
DOI 10.1111/j.1600-0854.2012.01341.x, PubMed 22321063
Publications 2011
T cells raised against allogeneic HLA-A2/CD20 kill primary follicular lymphoma and acute lymphoblastic leukemia cells
130 (8), 1821-32
DOI 10.1002/ijc.26209, PubMed 21630262
A single point mutation in ricin A-chain increases toxin degradation and inhibits EDEM1-dependent ER retrotranslocation
436 (2), 371-85
DOI 10.1042/BJ20101493, PubMed 21388347
A practical approach to T-cell receptor cloning and expression
6 (11), e27930
DOI 10.1371/journal.pone.0027930, PubMed 22132171
Publications 2010
Targeting B cell leukemia with highly specific allogeneic T cells with a public recognition motif
24 (11), 1901-9
DOI 10.1038/leu.2010.186, PubMed 20844564
Transiently redirected T cells for adoptive transfer
13 (5), 629-40
DOI 10.3109/14653249.2010.542461, PubMed 21174490
Splice variants of enigma homolog, differentially expressed during heart development, promote or prevent hypertrophy
86 (3), 374-82
DOI 10.1093/cvr/cvq023, PubMed 20097676
Publications 2009
Glycosphingolipid requirements for endosome-to-Golgi transport of Shiga toxin
10 (7), 868-82
DOI 10.1111/j.1600-0854.2009.00919.x, PubMed 19453975
SNX4 in complex with clathrin and dynein: implications for endosome movement
4 (6), e5935
DOI 10.1371/journal.pone.0005935, PubMed 19529763
β-arrestins attenuate p38-mediated endosome to Golgi transport
11 (5), 796-807
DOI 10.1111/j.1462-5822.2009.01292.x, PubMed 19159388
Dendritic cells engineered to express defined allo-HLA peptide complexes induce antigen-specific cytotoxic T cells efficiently killing tumour cells
69 (4), 319-28
DOI 10.1111/j.1365-3083.2008.02223.x, PubMed 19284496
Characterization of clathrin and Syk interaction upon Shiga toxin binding
21 (7), 1161-8
DOI 10.1016/j.cellsig.2009.03.005, PubMed 19289168
Publications 2008
Enigma homolog 1 scaffolds protein kinase D1 to regulate the activity of the cardiac L-type voltage-gated calcium channel
78 (3), 458-65
DOI 10.1093/cvr/cvn052, PubMed 18296710
beta-catenin is involved in N-cadherin-dependent adhesion, but not in canonical Wnt signaling in E2A-PBX1-positive B acute lymphoblastic leukemia cells
37 (2), 225-33
DOI 10.1016/j.exphem.2008.10.007, PubMed 19101069
Phosphorylation of fibroblast growth factor (FGF) receptor 1 at Ser777 by p38 mitogen-activated protein kinase regulates translocation of exogenous FGF1 to the cytosol and nucleus
28 (12), 4129-41
DOI 10.1128/MCB.02117-07, PubMed 18411303
Vector-based delivery of siRNAs: in vitro and in vivo challenges
13, 3488-93
DOI 10.2741/2943, PubMed 18508450
Vector-based delivery of siRNAs: In vitro and in vivo challenges
Front. Biosci., 13, 3488-3493
Publications 2007
Axonal guidance protein FEZ1 associates with tubulin and kinesin motor protein to transport mitochondria in neurites of NGF-stimulated PC12 cells
361 (3), 605-10
DOI 10.1016/j.bbrc.2007.07.050, PubMed 17669366
Phosphoinositide-regulated retrograde transport of ricin: crosstalk between hVps34 and sorting nexins
8 (3), 297-309
DOI 10.1111/j.1600-0854.2006.00527.x, PubMed 17319803
Protein kinase Cdelta is activated by Shiga toxin and regulates its transport
282 (22), 16317-28
DOI 10.1074/jbc.M610886200, PubMed 17403690
The Mitogen-activated protein kinase p38 links Shiga Toxin-dependent signaling and trafficking
19 (1), 95-104
DOI 10.1091/mbc.e07-06-0565, PubMed 17959827
Publications 2006
The ESCRT-III subunit hVps24 is required for degradation but not silencing of the epidermal growth factor receptor
17 (6), 2513-23
DOI 10.1091/mbc.e05-10-0915, PubMed 16554368
Transcriptional targeting of small interfering RNAs into cancer cells
350 (4), 854-9
DOI 10.1016/j.bbrc.2006.09.127, PubMed 17034763
EDEM is involved in retrotranslocation of ricin from the endoplasmic reticulum to the cytosol
17 (4), 1664-75
DOI 10.1091/mbc.e05-10-0961, PubMed 16452630
Transport of ricin from endosomes to the Golgi apparatus is regulated by Rab6A and Rab6A'
7 (6), 663-72
DOI 10.1111/j.1600-0854.2006.00418.x, PubMed 16683916
Publications 2005
Golgi vesiculation induced by cholesterol occurs by a dynamin- and cPLA2-dependent mechanism
6 (2), 144-56
DOI 10.1111/j.1600-0854.2005.00258.x, PubMed 15634214
The disulfide isomerase Grp58 is a protective factor against prion neurotoxicity
25 (11), 2793-802
DOI 10.1523/JNEUROSCI.4090-04.2005, PubMed 15772339
Shiga toxin regulates its entry in a Syk-dependent manner
17 (3), 1096-109
DOI 10.1091/mbc.e05-08-0766, PubMed 16371508
Strategies for the design of random siRNA libraries and the selection of anti-GFP siRNAs
309, 83-91
DOI 10.1385/1-59259-935-4:083, PubMed 15990399
Reconstitution of active diphtheria toxin based on a hexahistidine tagged version of the B-fragment produced to high yields in bacteria
46 (8), 900-6
DOI 10.1016/j.toxicon.2005.08.015, PubMed 16260019
Sap-1/PTPRH activity is regulated by reversible dimerization
331 (2), 497-502
DOI 10.1016/j.bbrc.2005.03.196, PubMed 15850787
Publications 2003
Mapping of synergistic components of weakly interacting protein-protein motifs using arrays of paired peptides
278 (17), 15162-7
DOI 10.1074/jbc.M211887200, PubMed 12551909
Identification of protein tyrosine phosphatases with specificity for the ligand-activated growth hormone receptor
17 (11), 2228-39
DOI 10.1210/me.2003-0011, PubMed 12907755
PTPH1 is a predominant protein-tyrosine phosphatase capable of interacting with and dephosphorylating the T cell receptor zeta subunit
279 (9), 7760-9
DOI 10.1074/jbc.M309994200, PubMed 14672952
Probing protein-tyrosine phosphatase substrate specificity using a phosphotyrosine-containing phage library
279 (1), 311-8
DOI 10.1074/jbc.M307617200, PubMed 14578355
Publications 2002
Protein tyrosine phosphatases as drug targets: PTP1B and beyond
6 (6), 637-47
DOI 10.1517/14728222.6.6.637, PubMed 12472377
Publications 2001
Pulling strings below the surface: hormone receptor signaling through inhibition of protein tyrosine phosphatases
15 (1), 19-28
DOI 10.1385/endo:15:1:019, PubMed 11572321
Publications 2000
MetaBlasts: tracing protein tyrosine phosphatase gene family roots from Man to Drosophila melanogaster and Caenorhabditis elegans genomes
253 (2), 137-43
DOI 10.1016/s0378-1119(00)00275-4, PubMed 10940550
Identification of tyrosine phosphatases that dephosphorylate the insulin receptor. A brute force approach based on "substrate-trapping" mutants
275 (13), 9792-6
DOI 10.1074/jbc.275.13.9792, PubMed 10734133