We assist researchers with the production, characterization and differentiation of human pluripotent stem cells

The Core Facility for Human Pluripotent Stem Cells offers services related to research on human ES and iPS cells. We provide reprogramming of iPS cell lines from patients and healthy donors, for example for in vitro disease modeling. We have already-established human ES cell lines and a large bank of human iPS cell lines established through ongoing REK-approved projects. 

Our services include:

• reprogramming of hiPS cells from skin biopsies and blood samples
• characterization of hES and hiPS cells (expression of pluripotency markers, embryoid body formation)
• training and assistance in cultivation of hES and hiPS cells
• long-term storage of skin fibroblasts and hiPS cells
• mycoplasma testing (not restricted to hES or hiPS cells)
• use of laboratory space including a quarantine lab, a BS2-classified virus lab, and a fully contained variable oxygen cell cultivation unit
• differentiation of selected cell types from hES and hiPS cells
• strategic planning of reprogramming experiments and in vitro disease modeling
• cell characterization equipment (qPCR, flow cytometry and user-available confocal microscope)
• Gene editing (CRISPR), knock-in, knock-out, SNP correction, and reporter iPSC generation

See a detailed list of services and pricing here.

To request services at the Norwegian Stem Cell Core Facility please use the REQUEST FORM and submit it to corefacility@medisin.uio.no

Location

We are located on the first floor of Domus Medica, across the street from the main National Hospital building.

International network

The Norwegian Core Facility for Human Pluripotent Stem Cells represents Norway in the following international core facility consortia:

Stem Cell COREdinates

Human Pluripotent Stem Cell Registry - hPSCreg 

International Stem Cell Banking Initiative - ISCBI

EU COST Action CorEuStem - The European Network for Stem Cell Core Facilities 

EU COST Action HAPLOiPS - Generation of Human Induced Pluripotent Stem Cells From Haplo-selected Cord Blood Samples

We consult regularly with Leiden University Medical School hiPSC Core Facility and Harvard Stem Cell Institute iPS Core Facility on technological developments in iPS cell reprogramming and characterization.

Facilities and equipment

Available equipment at the Norwegian Core Facility for Human Pluripotent Stem Cells:

• 140 m2 lab space
• 4 fully equipped cell culture rooms (one with BS2 designation for use of viruses)
• Cryopreservation tanks for storage of human iPS cells
• Flow cytometry unit (Beckman Coulter Gallios 3-laser)
• Real-Time qPCR System (Step-One Plus from Applied Biosytems)
• Confocal microscope (LSM-700 from Zeiss)
• Fully contained variable oxygen cell cultivation unit (BioSpherix)

Core Facility Staff

	Hege Brincker Fjerdingstad Daily Manager Research Engineer Email +47 22851578 / 92287650
	Helena Lading Research Engineer

For inquiries please contact corefacility@medisin.uio.no.

Current Users: here

Norwegian Core Facility Area and Equipment:        

Gallios analytical flow cytometry unit from Beckman Coulter (10 colors/3 lasers)

                
Virus Lab (Biosafety Level 2)                      ES Outer Lab 

 
ES Inner Lab

 
Zeiss LSM 700 laser scanning confocal microscope with enviromentally controlled cell culture chamber

EnvisionTech 3D Bioplotter

BioSpherix X3 Fully-contained multichambered controlled atmosphere cell culture and incubation unit