Norwegian Core Facility for human pluripotent stem cell production and characterization

The Norwegian Core Facility for Human Pluripotent Stem Cells supplies researchers throughout Norway with access to established human ES and iPS cell lines and also the technical services and instruction required for the derivation of iPS cells and the characterization of ES and iPS cell-derived cells. Researchers can obtain ES and iPS cell-related services or make use of the facility to differentiate and characterize cells themselves. The Norwegian Core Facility has a staff of 3 highly trained cell culture technicians and a laboratory technician, and is supported by the SouthEast Regional Health Authority (HSØ).

The Norwegian Core Facility is located on the first floor of Domus Medica in close proximity to the Center's research groups. It houses 140 m² of lab space with 4 fully equipped cell culture rooms (one of which has S2 designation for culture of virus-producing cells), cryopreservation tanks for storage of human pluripotent stem cells, a flow cytometry unit (Beckman Coulter Gallios 3-laser), a Real-Time PCR System (Step-One Plus from Applied Biosytems) and imaging equipment for stem cell characterization (Confocal microscope LSM-700 from Zeiss). 

With this instrument park, the Core Facility can provide services related to reprogramming, characterization, differentiation and storage of human pluripotent stem cells, environmental control of pluripotent stem cells and their derivatives in vitro, and highly sophisticated 3D tissue construction.

The Core Facility is being used regularly by Center groups and other users for hES cell culture work, iPS cell generation, mycoplasma testing, lentivirus production and transduction, flow cytometry, cell sorting and confocal microscopy. For booking system and list of prices, follow the links listed below:

 

Current Users: here

Available Services: here

Booking system for registered users: here

 

Users who wish to engage in hES or hiPS cell-related projects will need to meet with the Core Facility management before initiating the project to discuss appropriate strategy and logistics.

 

Request Services:

To request services at the Norwegian Stem Cell Core Facility please use the REQUEST FORM and submit it to corefacility@medisin.uio.no

 

 

Norwegian Core Facililty Staff:

Hege Brincker-Fjerdingstad

Hege Brincker Fjerdingstad
Daily Manager
Research Engineer

Email
+47 22851578 / 92287650

 

  

Kristin Buchholz

 Kirstin Franziska Buchholz 
 Research Engineer

May Laura Kilano Khider  May Laura Kilano Khider
 Research Engineer

For inquiries please contact corefacility@medisin.uio.no.

 

Norwegian Core Facility Area and Equipment:        

Flow cytometry at the core facility
Gallios analytical flow cytometry unit from Beckman Coulter (10 colors/3 lasers)

 

Virus lab               ES outer lab 
Virus Lab (Biosafety Level 2)                      ES Outer Lab 

 

ES inner lab 
ES Inner Lab

 

Confocal Microscope 
Zeiss Meta 700 laser scanning confocal microscope with enviromentally controlled cell culture chamber

 

EnvisionTech 3D Bioplotter
EnvisionTech 3D Bioplotter

 

BioSpherix X3 Variable Oxygen Cell Culture Unit
BioSpherix X3 Variable Oxygen Cell Culture Unit

 
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