Norwegian Core Facility for human pluripotent stem cell production and characterization
We assist researchers with the production, characterization and differentiation of human pluripotent stem cells
Services
The Core Facility for Human Pluripotent Stem Cells offers services related to research on human ES and iPS cells. We provide reprogramming of iPS cell lines from patients and healthy donors, for example for in vitro disease modeling. We have already-established human ES cell lines and a large bank of human iPS cell lines established through ongoing REK-approved projects.
Our services include:
- reprogramming of hiPS cells from skin biopsies and blood samples
- characterization of hES and hiPS cells (expression of pluripotency markers, embryoid body formation)
- training and assistance in cultivation of hES and hiPS cells
- long-term storage of skin fibroblasts and hiPS cells
- mycoplasma testing (not restricted to hES or hiPS cells)
- use of laboratory space including a quarantine lab, a BS2-classified virus lab, and a fully contained variable oxygen cell cultivation unit
- differentiation of selected cell types from hES and hiPS cells
- strategic planning of reprogramming experiments and in vitro disease modeling
- cell characterization equipment (qPCR, flow cytometry and user-available confocal microscope)
- Gene editing (CRISPR), knock-in, knock-out, SNP correction, and reporter iPSC generation
See a detailed list of services and pricing here.
To request services at the Norwegian Stem Cell Core Facility please use the REQUEST FORM and submit it to corefacility@medisin.uio.no
Location
We are located on the first floor of Domus Medica, across the street from the main National Hospital building.
International network
The Norwegian Core Facility for Human Pluripotent Stem Cells represents Norway in the following international core facility consortia:
Human Pluripotent Stem Cell Registry - hPSCreg
International Stem Cell Banking Initiative - ISCBI
EU COST Action CorEuStem - The European Network for Stem Cell Core Facilities
We consult regularly with Leiden University Medical School hiPSC Core Facility and Harvard Stem Cell Institute iPS Core Facility on technological developments in iPS cell reprogramming and characterization.
Facilities and equipment
Available equipment at the Norwegian Core Facility for Human Pluripotent Stem Cells:
- 140 m2 lab space
- 4 fully equipped cell culture rooms (one with BS2 designation for use of viruses)
- Cryopreservation tanks for storage of human iPS cells
- Flow cytometry unit (Beckman Coulter Gallios 3-laser)
- Real-Time qPCR System (Step-One Plus from Applied Biosytems)
- Confocal microscope (LSM-700 from Zeiss)
- Fully contained variable oxygen cell cultivation unit (BioSpherix)
Core Facility Staff
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Hege Brincker Fjerdingstad |
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Helena Lading Research Engineer
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For inquiries please contact corefacility@medisin.uio.no.
Current Users: here
Norwegian Core Facility Area and Equipment:
Gallios analytical flow cytometry unit from Beckman Coulter (10 colors/3 lasers)
Virus Lab (Biosafety Level 2) ES Outer Lab
ES Inner Lab
Zeiss LSM 700 laser scanning confocal microscope with enviromentally controlled cell culture chamber
EnvisionTech 3D Bioplotter
BioSpherix X3 Fully-contained multichambered controlled atmosphere cell culture and incubation unit