Microsatellite instability (MSI) studies

Tumors with a defect mismatch repair system show MSI.

Two mononucleotide repeats, BAT25 and BAT26, identify more than 99% of the tumors with MSI (Hoang et al., 1997; Zhou et al., 1998).



Figure: Each forward primer was end labeled with a fluorochrome and the primer sets were run in duplex PCR. The PCR products were separated by capillary electrophoresis on an ABI PRISMTM310 Genetic Analyzer.
Figure: Each forward primer was end labeled with a fluorochrome and the primer sets were run in duplex PCR. The PCR products were separated by capillary electrophoresis on an ABI PRISMTM310 Genetic Analyzer.

Selected publications regarding microsatellite instability:

PubMed Diep CB, Thorstensen L, Meling GI, Skovlund E, Rognum TO, and Lothe RA (2003). Genetic tumor markers with prognostic impact in Dukes' stages B and C colorectal cancer patients. J.Clin.Oncol., 21(5): 820-829.

PubMed Thorstensen L, Diep CB, Meling GI, Aagesen TH, Ahrens CH, Rognum TO, and Lothe RA (2001). WNT1 inducible signaling pathway protein 3, WISP-3, a novel target gene in colorectal carcinomas with microsatellite instability. Gastroenterology, 121(6): 1275-1280.

PubMed Lothe RA, Peltomäki P, Meling GI, Aaltonen LA, Nystrom-Lahti M, Pylkkanen L, Heimdal K, Andersen TI, Moller P, and Rognum TO (1993). Genomic instability in colorectal cancer: relationship to clinicopathological variables and family history. Cancer Res., 53(24): 5849-5852.

PubMed Peltomäki P, Lothe RA, Aaltonen LA, Pylkkanen L, Nystrom-Lahti M, Seruca R, David L, Holm R, Ryberg D, and Haugen A (1993). Microsatellite instability is associated with tumors that characterize the hereditary non-polyposis colorectal carcinoma syndrome. Cancer Res., 53(24): 5853-5855.
 
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