The next institute seminar takes place Wednesday October 21st at 12:00 in the Auditorium with the following speaker:
Kanutte Huse – Dept. of Core Facilities
Title of her talk:
“Mass cytometry - basics and applications”
Mass cytometry (CyTOF) is a variation of flow cytometry in which antibodies are labeled with metal isotopes rather than fluorochromes, using time-of-flight mass spectrometry as read-out. This allows for the combination of many more antibody specificities in a single sample, without significant spillover between channels. A CyTOF2 is now being installed at the Flow Cytometry Core Facility. I will present the basics and some applications of this technology. I will also present viSNE, a computational tool that allows one to map high-dimensional cytometry data onto two dimensions, yet conserve the high-dimensional structure of the data.