Norwegian Core Facility Available Services

LIST OF SERVICES

COSTS (VAT incl. for OUS users, VAT excl. for external users)

 Use of culture facility

  Fee-based by agreement, booking required
 Use of virus lab Fee-based by agreement, booking required
 Training in hiPSC cultivation 6000 NOK /person or 9000 NOK /two people, -  Reagenses not included
 Quarantine cell culturing prior
 to mycoplasma testing		 100 NOK /day, booking required
 Use of confocal microscope  100 NOK /hour, booking required
 Human Feeders (irradiated)  20 NOK /35 mm dish
 hESC / hiPSC cultivation  Negotiable based on volume 

 hiPSC generation (five clones depending on donor cell quality) from skin biopsy and blood
     - banked fibroblast line from skin biopsy
     - reprogrammed hiPSC line (Sendai virus)
     - reprogrammed hiPSC line (ReproRNA-OKSGM)


                                                                                                                                  1 000 NOK /line (2 cryotubes/biopsy)         
  35 000 NOK /donor (4-5 iPSC lines/donor)        
  35 000 NOK /donor (4-5 iPSC lines/donor)

 Gene editing (CRISPR)                                                                         - Knock Out editing
 - Knock-in generation
 - Point mutation (SNP) correction/insertion                                   - Reporter cell line generation


 

 

  60 000 NOK /line         
  70 000 NOK /line        
  70 000 NOK /line                                                                                                70-75 000 NOK /line (depending on the size of the reporter)                       

Five vials of two edited lines (0.5 mill. cells/vial) will be delivered

 Media/reagents
     - hESC medium

 

1000 NOK /500 mL
 Mycoplasma detection in cell culture media  300 NOK /test
 Cultivation of in-house /user provided hESC and hiPSCs  Negotiable based on volume
 Storage (liquid nitrogen) of hESC and hiPSCs  1000 NOK /year
 Pluripotency testing of user-provided cells
     - qPCR
     - immunocytochemistry and confocal microscopy

 

  400 NOK /plate (perishables fee-based by agreement)
  1000 NOK /line

 Differentiation via EB to three germ layers

  2500 NOK /line

 Genetic karyotyping of hES and hiPSCs

  2500 NOK /line

 Differentiation via hiPSCs to human neural progenitor cells (hNPCS)

  3500 NOK/line

 Flow cytometry
      - Cell analysis
      - DNA analysis
     

  
  50 NOK / sample
  100 NOK / sample
 

Gene editing (CRISPR), knock-in, knock-out, SNP correction, and reporter iPSC generation

 

Negotiable based on service

 Immunostaining of differentiated hESC and hiPSCs

 Negotiable based on volume

 Use of Variable Oxygen Cell Culture Unit (Biospherix)

         - Gas supply (user dependent)

 

500 NOK / 50 L nitrogen gas
250 NOK / day
1000 NOK / week
3500 NOK / month
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