Alicia Llorente
- Project Leader, Senior Scientist (Helse Sør-øst)
- +47 22 78 18 27
Alicia is in charge of the project Exosomes and Prostate Cancer
From the news:
Llorente and coworkers report promising prostate cancer biomarkers in urinary exosomes
Innovation projects funded by the Norwegian Cancer Society and Research Council
Alicia Llorente participates in an ERA-NET funded project
Publications 2024
From sweat to hope: The role of exercise-induced extracellular vesicles in cancer prevention and treatment
J Extracell Vesicles, 13 (8), e12500
DOI 10.1002/jev2.12500, PubMed 39183543
Hippo and PI5P4K signaling intersect to control the transcriptional activation of YAP
Sci Signal, 17 (838), eado6266
DOI 10.1126/scisignal.ado6266, PubMed 38805583
Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches
J Extracell Vesicles, 13 (2), e12404
DOI 10.1002/jev2.12404, PubMed 38326288
Publications 2023
Plasma and urinary extracellular vesicles as a source of RNA biomarkers for prostate cancer in liquid biopsies
Front Mol Biosci, 10, 980433
DOI 10.3389/fmolb.2023.980433, PubMed 36818049
siRNA screening reveals that SNAP29 contributes to exosome release
Cell Mol Life Sci, 80 (7), 177
DOI 10.1007/s00018-023-04822-8, PubMed 37285022
Lipid Biomarkers in Liquid Biopsies: Novel Opportunities for Cancer Diagnosis
Pharmaceutics, 15 (2)
DOI 10.3390/pharmaceutics15020437, PubMed 36839759
The expanding organelle lipidomes: current knowledge and challenges
Cell Mol Life Sci, 80 (8), 237
DOI 10.1007/s00018-023-04889-3, PubMed 37530856
Lipids in Extracellular Vesicles: What Can Be Learned about Membrane Structure and Function?
Cold Spring Harb Perspect Biol, 15 (8)
DOI 10.1101/cshperspect.a041415, PubMed 37277192
The quick reference card "Storage of urinary EVs" - A practical guideline tool for research and clinical laboratories
J Extracell Vesicles, 12 (3), e12286
DOI 10.1002/jev2.12286, PubMed 36916183
Publications 2022
Extracellular Vesicles as Novel Players in Kidney Disease
J Am Soc Nephrol, 33 (3), 467-471
DOI 10.1681/ASN.2021091232, PubMed 35131841
Extracellular vesicles and microRNAs are altered in response to exercise, insulin sensitivity and overweight
Acta Physiol (Oxf), 236 (4), e13862
DOI 10.1111/apha.13862, PubMed 36377504
PIP kinases: A versatile family that demands further therapeutic attention
Adv Biol Regul, 87, 100939
DOI 10.1016/j.jbior.2022.100939, PubMed 36517396
Amniotic fluid stem cell-derived extracellular vesicles are independent metabolic units capable of modulating inflammasome activation in THP-1 cells
FASEB J, 36 (4), e22218
DOI 10.1096/fj.202101657R, PubMed 35218567
Biodistribution, pharmacokinetics and excretion studies of intravenously injected nanoparticles and extracellular vesicles: Possibilities and challenges
Adv Drug Deliv Rev, 186, 114326
DOI 10.1016/j.addr.2022.114326, PubMed 35588953
Publications 2021
Uptake of circulating extracellular vesicles from rectal cancer patients and differential responses by human monocyte cultures
FEBS Open Bio, 11 (3), 724-740
DOI 10.1002/2211-5463.13098, PubMed 33512765
Urinary extracellular vesicles: A position paper by the Urine Task Force of the International Society for Extracellular Vesicles
J Extracell Vesicles, 10 (7), e12093
DOI 10.1002/jev2.12093, PubMed 34035881
Implication of β2-adrenergic receptor and miR-196a correlation in neurite outgrowth of LNCaP prostate cancer cells
PLoS One, 16 (6), e0253828
DOI 10.1371/journal.pone.0253828, PubMed 34191854
Ebulin l Is Internalized in Cells by Both Clathrin-Dependent and -Independent Mechanisms and Does Not Require Clathrin or Dynamin for Intoxication
Toxins (Basel), 13 (2)
DOI 10.3390/toxins13020102, PubMed 33573355
Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)1
Autophagy, 17 (1), 1-382
DOI 10.1080/15548627.2020.1797280, PubMed 33634751
Extracellular Vesicles and Renal Fibrosis: An Odyssey toward a New Therapeutic Approach
Int J Mol Sci, 22 (8)
DOI 10.3390/ijms22083887, PubMed 33918699
Urinary Extracellular Vesicles in Urology: Current Successes and Challenges Ahead
Eur Urol, 81 (1), 127-128
DOI 10.1016/j.eururo.2021.09.025, PubMed 34625312
Extracellular vesicles as a source of prostate cancer biomarkers in liquid biopsies: a decade of research
Br J Cancer, 126 (3), 331-350
DOI 10.1038/s41416-021-01610-8, PubMed 34811504
Potential of miRNAs in urinary extracellular vesicles for management of active surveillance in prostate cancer patients
Br J Cancer, 126 (3), 492-501
DOI 10.1038/s41416-021-01598-1, PubMed 34811506
Publications 2020
Mass spectrometry for the identification of protein biomarkers in urinary extracellular vesicles
In Proteomic and Metabolomic Approaches to Biomarker Discovery (Second Edition) (Issaq HJ, Veenstra TD, eds.), Academic Press , Chapter 26, 437-440
DOI 10.1016/B978-0-12-818607-7.00026-8, PublikaID 335, ISBN 978-0-12-818607-7
Nanoparticle-based biosensors for detection of extracellular vesicles in liquid biopsies
J Mater Chem B, 8 (31), 6710-6738
DOI 10.1039/d0tb00861c, PubMed 32627783
Adult Stem Cell-Derived Extracellular Vesicles in Cancer Treatment: Opportunities and Challenges
Cells, 9 (5)
DOI 10.3390/cells9051171, PubMed 32397238
An emerging focus on lipids in extracellular vesicles
Adv Drug Deliv Rev, 159, 308-321
DOI 10.1016/j.addr.2020.03.002, PubMed 32151658
ERK1/2 Signaling Induces Upregulation of ANGPT2 and CXCR4 to Mediate Liver Metastasis in Colon Cancer
Cancer Res, 80 (21), 4668-4680
DOI 10.1158/0008-5472.CAN-19-4028, PubMed 32816905
Analytical techniques for multiplex analysis of protein biomarkers
Expert Rev Proteomics, 17 (4), 257-273
DOI 10.1080/14789450.2020.1763174, PubMed 32427033
Towards defining reference materials for measuring extracellular vesicle refractive index, epitope abundance, size and concentration
J Extracell Vesicles, 9 (1), 1816641
DOI 10.1080/20013078.2020.1816641, PubMed 33062218
Publications 2019
Biological membranes in EV biogenesis, stability, uptake, and cargo transfer: an ISEV position paper arising from the ISEV membranes and EVs workshop
J Extracell Vesicles, 8 (1), 1684862
DOI 10.1080/20013078.2019.1684862, PubMed 31762963
Publications 2018
Summary of the ISEV workshop on extracellular vesicles as disease biomarkers, held in Birmingham, UK, during December 2017
J Extracell Vesicles, 7 (1), 1473707
DOI 10.1080/20013078.2018.1473707, PubMed 31162490
Effect of colorectal cancer-derived extracellular vesicles on the immunophenotype and cytokine secretion profile of monocytes and macrophages
Cell Commun Signal, 16 (1), 17
DOI 10.1186/s12964-018-0229-y, PubMed 29690889
Exosomal lipid composition and the role of ether lipids and phosphoinositides in exosome biology
J Lipid Res, 60 (1), 9-18
DOI 10.1194/jlr.R084343, PubMed 30076207
Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines
J Extracell Vesicles, 7 (1), 1535750
DOI 10.1080/20013078.2018.1535750, PubMed 30637094
Publications 2017
Detection of circulating miRNAs: comparative analysis of extracellular vesicle-incorporated miRNAs and cell-free miRNAs in whole plasma of prostate cancer patients
BMC Cancer, 17 (1), 730
DOI 10.1186/s12885-017-3737-z, PubMed 29121858
Current knowledge on exosome biogenesis and release
Cell Mol Life Sci, 75 (2), 193-208
DOI 10.1007/s00018-017-2595-9, PubMed 28733901
A novel community driven software for functional enrichment analysis of extracellular vesicles data
J Extracell Vesicles, 6 (1), 1321455
DOI 10.1080/20013078.2017.1321455, PubMed 28717418
Identification of non-invasive miRNAs biomarkers for prostate cancer by deep sequencing analysis of urinary exosomes
Mol Cancer, 16 (1), 156
DOI 10.1186/s12943-017-0726-4, PubMed 28982366
A novel 3D heterotypic spheroid model for studying extracellular vesicle-mediated tumour and immune cell communication
Biochem Biophys Res Commun, 495 (2), 1930-1935
DOI 10.1016/j.bbrc.2017.12.072, PubMed 29248729
Lipids in exosomes: Current knowledge and the way forward
Prog Lipid Res, 66, 30-41
DOI 10.1016/j.plipres.2017.03.001, PubMed 28342835
Size and concentration analyses of extracellular vesicles by nanoparticle tracking analysis: a variation study
J Extracell Vesicles, 6 (1), 1344087
DOI 10.1080/20013078.2017.1344087, PubMed 28804597
Publications 2016
Diagnostic, prognostic and predictive value of cell-free miRNAs in prostate cancer: a systematic review
Mol Cancer, 15 (1), 41
DOI 10.1186/s12943-016-0523-5, PubMed 27189160
Evidence-Based Clinical Use of Nanoscale Extracellular Vesicles in Nanomedicine
ACS Nano, 10 (4), 3886-99
DOI 10.1021/acsnano.5b08015, PubMed 26978483
PIKfyve inhibition increases exosome release and induces secretory autophagy
Cell Mol Life Sci, 73 (24), 4717-4737
DOI 10.1007/s00018-016-2309-8, PubMed 27438886
Data including GROMACS input files for atomistic molecular dynamics simulations of mixed, asymmetric bilayers including molecular topologies, equilibrated structures, and force field for lipids compatible with OPLS-AA parameters
Data Brief, 7, 1171-1174
DOI 10.1016/j.dib.2016.03.067, PubMed 27761499
Molecular lipid species in urinary exosomes as potential prostate cancer biomarkers
Eur J Cancer, 70, 122-132
DOI 10.1016/j.ejca.2016.10.011, PubMed 27914242
Exosomal proteins as prostate cancer biomarkers in urine: From mass spectrometry discovery to immunoassay-based validation
Eur J Pharm Sci, 98, 80-85
DOI 10.1016/j.ejps.2016.09.023, PubMed 27664330
Publications 2015
Interdigitation of long-chain sphingomyelin induces coupling of membrane leaflets in a cholesterol dependent manner
Biochim Biophys Acta, 1858 (2), 281-8
DOI 10.1016/j.bbamem.2015.12.003, PubMed 26654782
Biological properties of extracellular vesicles and their physiological functions
J Extracell Vesicles, 4, 27066
DOI 10.3402/jev.v4.27066, PubMed 25979354
Identification of prostate cancer biomarkers in urinary exosomes
Oncotarget, 6 (30), 30357-76
DOI 10.18632/oncotarget.4851, PubMed 26196085
Publications 2014
Regulation of exosome release by glycosphingolipids and flotillins
FEBS J, 281 (9), 2214-27
DOI 10.1111/febs.12775, PubMed 24605801
The ether lipid precursor hexadecylglycerol stimulates the release and changes the composition of exosomes derived from PC-3 cells
J Biol Chem, 290 (7), 4225-37
DOI 10.1074/jbc.M114.593962, PubMed 25519911
Publications 2013
Exosomal miRNAs as Biomarkers for Prostate Cancer
Front Genet, 4, 36
DOI 10.3389/fgene.2013.00036, PubMed 23519132
Molecular lipidomics of exosomes released by PC-3 prostate cancer cells
Biochim Biophys Acta, 1831 (7), 1302-9
DOI 10.1016/j.bbalip.2013.04.011, PubMed 24046871
Publications 2012
Profiling of microRNAs in exosomes released from PC-3 prostate cancer cells
Biochim Biophys Acta, 1819 (11-12), 1154-63
DOI 10.1016/j.bbagrm.2012.08.016, PubMed 22982408
Vesiclepedia: a compendium for extracellular vesicles with continuous community annotation
PLoS Biol, 10 (12), e1001450
DOI 10.1371/journal.pbio.1001450, PubMed 23271954
Proteomic analysis of microvesicles released by the human prostate cancer cell line PC-3
Mol Cell Proteomics, 11 (7), M111.012914
DOI 10.1074/mcp.M111.012914, PubMed 22457534
Publications 2007
Structural requirements for furin-induced cleavage and activation of Shiga toxin
Biochem Biophys Res Commun, 357 (1), 144-9
DOI 10.1016/j.bbrc.2007.03.110, PubMed 17407762
Cholesterol regulates prostasome release from secretory lysosomes in PC-3 human prostate cancer cells
Eur J Cell Biol, 86 (7), 405-15
DOI 10.1016/j.ejcb.2007.05.001, PubMed 17601634
Polyunsaturated fatty acids regulate Shiga toxin transport
Biochem Biophys Res Commun, 364 (2), 283-8
DOI 10.1016/j.bbrc.2007.09.126, PubMed 17942073
Publications 2004
Caveolin-1 and MAL are located on prostasomes secreted by the prostate cancer PC-3 cell line
J Cell Sci, 117 (Pt 22), 5343-51
DOI 10.1242/jcs.01420, PubMed 15466889
Publications 2003
Induction of direct endosome to endoplasmic reticulum transport in Chinese hamster ovary (CHO) cells (LdlF) with a temperature-sensitive defect in epsilon-coatomer protein (epsilon-COP)
J Biol Chem, 278 (37), 35850-5
DOI 10.1074/jbc.M303425200, PubMed 12847103
Publications 2002
Endosome-to-Golgi transport is regulated by protein kinase A type II alpha
J Biol Chem, 278 (3), 1991-7
DOI 10.1074/jbc.M209982200, PubMed 12419802
Selective regulation of the Rab9-independent transport of ricin to the Golgi apparatus by calcium
J Cell Sci, 115 (Pt 17), 3449-56
DOI 10.1242/jcs.115.17.3449, PubMed 12154075
Publications 2001
Endosome to Golgi transport of ricin is independent of clathrin and of the Rab9- and Rab11-GTPases
Mol Biol Cell, 12 (7), 2099-107
DOI 10.1091/mbc.12.7.2099, PubMed 11452006
Proteoglycan synthesis is increased in cells with impaired clathrin-dependent endocytosis
J Cell Sci, 114 (Pt 2), 335-43
DOI 10.1242/jcs.114.2.335, PubMed 11148135
Publications 2000
Studies of the endocytic pathway: analysis of ricin trafficking
Department Group for Laboratory Medicine, Faculty of Medicine, University of Oslo, Oslo, 1 b. (flere pag.)
BIBSYS 001842501, ISBN 82-7633-156-4
Apical endocytosis of ricin in MDCK cells is regulated by the cyclooxygenase pathway
J Cell Sci, 113 ( Pt 7), 1213-21
DOI 10.1242/jcs.113.7.1213, PubMed 10704372
Role for dynamin in late endosome dynamics and trafficking of the cation-independent mannose 6-phosphate receptor
Mol Biol Cell, 11 (2), 481-95
DOI 10.1091/mbc.11.2.481, PubMed 10679008
Apical macropinocytosis in polarized MDCK cells: regulation by N-ethylmaleimide-sensitive proteins
Eur J Cell Biol, 79 (7), 447-57
DOI 10.1078/0171-9335-00071, PubMed 10961444
Publications 1998
Expression of mutant dynamin inhibits toxicity and transport of endocytosed ricin to the Golgi apparatus
J Cell Biol, 140 (3), 553-63
DOI 10.1083/jcb.140.3.553, PubMed 9456316
Transport of apically but not basolaterally internalized ricin to the Golgi apparatus is stimulated by 8-Br-cAMP in MDCK cells
FEBS Lett, 431 (2), 200-4
DOI 10.1016/s0014-5793(98)00754-6, PubMed 9708902
Publications 1996
Effect of calmodulin antagonists on endocytosis and intracellular transport of ricin in polarized MDCK cells
Exp Cell Res, 227 (2), 298-308
DOI 10.1006/excr.1996.0279, PubMed 8831568