Antoni Wiedlocha
- Project Leader, Senior Scientist (Institute for Cancer Research)
- +47 22 78 18 05
Antoni is in charge of the project Protein internalisation and signaling
Publications 2023
Intracellular FGF1 protects cells from apoptosis through direct interaction with p53
Cell Mol Life Sci, 80 (10), 311
DOI 10.1007/s00018-023-04964-9, PubMed 37783936
Publications 2022
Strategies to inhibit FGFR4 V550L-driven rhabdomyosarcoma
Br J Cancer, 127 (11), 1939-1953
DOI 10.1038/s41416-022-01973-6, PubMed 36097178
Nuclear Localization Sequence of FGF1 Is Not Required for Its Intracellular Anti-Apoptotic Activity in Differentiated Cells
Cells, 11 (3)
DOI 10.3390/cells11030522, PubMed 35159330
FGF-FGFR signaling promotes the development of invasive cervical cancer
FEBS J, 289 (12), 3419-3421
DOI 10.1111/febs.16384, PubMed 35146913
Publications 2021
Fibroblast Growth Factor 2 Conjugated with Monomethyl Auristatin E Inhibits Tumor Growth in a Mouse Model
Biomacromolecules, 22 (10), 4169-4180
DOI 10.1021/acs.biomac.1c00662, PubMed 34542998
Effects of a Unique Combination of the Whole-Body Low Dose Radiotherapy with Inactivation of Two Immune Checkpoints and/or a Heat Shock Protein on the Transplantable Lung Cancer in Mice
Int J Mol Sci, 22 (12)
DOI 10.3390/ijms22126309, PubMed 34208396
Negative Regulation of FGFR (Fibroblast Growth Factor Receptor) Signaling
Cells, 10 (6)
DOI 10.3390/cells10061342, PubMed 34071546
Roles of the FGF-FGFR Signaling System in Cancer Development and Inflammation
Cells, 10 (9)
DOI 10.3390/cells10092231, PubMed 34571880
Publications 2020
FGF2-Derived PeptibodyF2-MMAE Conjugate for Targeted Delivery of Cytotoxic Drugs into Cancer Cells Overexpressing FGFR1
Cancers (Basel), 12 (10)
DOI 10.3390/cancers12102992, PubMed 33076489
Publications 2019
Cancer Mutations in FGFR2 Prevent a Negative Feedback Loop Mediated by the ERK1/2 Pathway
Cells, 8 (6)
DOI 10.3390/cells8060518, PubMed 31146385
Crosstalk between p38 and Erk 1/2 in Downregulation of FGF1-Induced Signaling
Int J Mol Sci, 20 (8)
DOI 10.3390/ijms20081826, PubMed 31013829
Publications 2018
Protein Tyrosine Phosphatase Receptor Type G (PTPRG) Controls Fibroblast Growth Factor Receptor (FGFR) 1 Activity and Influences Sensitivity to FGFR Kinase Inhibitors
Mol Cell Proteomics, 17 (5), 850-870
DOI 10.1074/mcp.RA117.000538, PubMed 29371290
Translocation of Exogenous FGF1 and FGF2 Protects the Cell against Apoptosis Independently of Receptor Activation
J Mol Biol, 430 (21), 4087-4101
DOI 10.1016/j.jmb.2018.08.004, PubMed 30099027
Publications 2017
Cytotoxic Conjugates of Fibroblast Growth Factor 2 (FGF2) with Monomethyl Auristatin E for Effective Killing of Cells Expressing FGF Receptors
ACS Omega, 2 (7), 3792-3805
DOI 10.1021/acsomega.7b00116, PubMed 30023704
Publications 2016
Proximity Labeling Reveals Molecular Determinants of FGFR4 Endosomal Transport
J Proteome Res, 15 (10), 3841-3855
DOI 10.1021/acs.jproteome.6b00652, PubMed 27615514
Publications 2015
Instability restricts signaling of multiple fibroblast growth factors
Cell Mol Life Sci, 72 (12), 2445-59
DOI 10.1007/s00018-015-1856-8, PubMed 25854632
The novel HSP90 inhibitor NVP-AUY922 shows synergistic anti-leukemic activity with cytarabine in vivo
Exp Cell Res, 340 (2), 220-6
DOI 10.1016/j.yexcr.2015.12.017, PubMed 26748184
Publications 2014
Nucleolin regulates phosphorylation and nuclear export of fibroblast growth factor 1 (FGF1)
PLoS One, 9 (3), e90687
DOI 10.1371/journal.pone.0090687, PubMed 24595027
Publications 2013
RSK2 regulates endocytosis of FGF receptor 1 by phosphorylation on serine 789
Oncogene, 33 (40), 4823-36
DOI 10.1038/onc.2013.425, PubMed 24141780
ERK-mediated phosphorylation of fibroblast growth factor receptor 1 on Ser777 inhibits signaling
Sci Signal, 6 (262), ra11
DOI 10.1126/scisignal.2003087, PubMed 23405013
Publications 2012
FGF1-gold nanoparticle conjugates targeting FGFR efficiently decrease cell viability upon NIR irradiation
Int J Nanomedicine, 7, 5915-27
DOI 10.2147/IJN.S36575, PubMed 23226697
Nuclear import of exogenous FGF1 requires the ER-protein LRRC59 and the importins Kpnα1 and Kpnβ1
Traffic, 13 (5), 650-64
DOI 10.1111/j.1600-0854.2012.01341.x, PubMed 22321063
Publications 2011
The driver of malignancy in KG-1a leukemic cells, FGFR1OP2-FGFR1, encodes an HSP90 addicted oncoprotein
Cell Signal, 23 (11), 1758-66
DOI 10.1016/j.cellsig.2011.06.010, PubMed 21745565
Translocation of exogenous FGF1 into cytosol and nucleus is a periodic event independent of receptor kinase activity
Exp Cell Res, 317 (7), 1005-15
DOI 10.1016/j.yexcr.2011.01.003, PubMed 21223966
Publications 2010
Roles of fibroblast growth factor receptors in carcinogenesis
Mol Cancer Res, 8 (11), 1439-52
DOI 10.1158/1541-7786.MCR-10-0168, PubMed 21047773
Publications 2009
Increased protein stability of FGF1 can compensate for its reduced affinity for heparin
J Biol Chem, 284 (37), 25388-403
DOI 10.1074/jbc.M109.001289, PubMed 19574212
Size limitation in translocation of fibroblast growth factor 1 fusion proteins across the endosomal membrane
Biochemistry, 48 (30), 7209-18
DOI 10.1021/bi9007353, PubMed 19558187
Publications 2008
Phosphorylation of fibroblast growth factor (FGF) receptor 1 at Ser777 by p38 mitogen-activated protein kinase regulates translocation of exogenous FGF1 to the cytosol and nucleus
Mol Cell Biol, 28 (12), 4129-41
DOI 10.1128/MCB.02117-07, PubMed 18411303
FGF-1: from biology through engineering to potential medical applications
Crit Rev Clin Lab Sci, 45 (1), 91-135
DOI 10.1080/10408360701713120, PubMed 18293181
Publications 2007
A nuclear export sequence located on a beta-strand in fibroblast growth factor-1
J Biol Chem, 282 (36), 26245-56
DOI 10.1074/jbc.M611234200, PubMed 17616529
Indirubin-3'-monoxime inhibits autophosphorylation of FGFR1 and stimulates ERK1/2 activity via p38 MAPK
Oncogene, 26 (44), 6372-85
DOI 10.1038/sj.onc.1210473, PubMed 17533378
Publications 2006
FGF-1 as a possible carrier for targeted drug delivery
Oncol Res, 16 (1), 27-34
DOI 10.3727/000000006783981242, PubMed 16783965
Functional diversity of FGF-2 isoforms by intracellular sorting
Bioessays, 28 (5), 504-14
DOI 10.1002/bies.20405, PubMed 16615083
Different abilities of the four FGFRs to mediate FGF-1 translocation are linked to differences in the receptor C-terminal tail
J Cell Sci, 119 (Pt 20), 4332-41
DOI 10.1242/jcs.03209, PubMed 17003104
FGF-1 and FGF-2 require the cytosolic chaperone Hsp90 for translocation into the cytosol and the cell nucleus
J Biol Chem, 281 (16), 11405-12
DOI 10.1074/jbc.M600477200, PubMed 16495214
Structural requirements of FGF-1 for receptor binding and translocation into cells
Biochemistry, 45 (51), 15338-48
DOI 10.1021/bi0618114, PubMed 17176056
Publications 2005
Two nuclear localization signals required for transport from the cytosol to the nucleus of externally added FGF-1 translocated into cells
Biochemistry, 44 (16), 6071-80
DOI 10.1021/bi047403m, PubMed 15835896
Highly stable mutants of human fibroblast growth factor-1 exhibit prolonged biological action
J Mol Biol, 352 (4), 860-75
DOI 10.1016/j.jmb.2005.07.066, PubMed 16126225
Publications 2004
Phosphorylation-regulated nucleocytoplasmic trafficking of internalized fibroblast growth factor-1
Mol Biol Cell, 16 (2), 794-810
DOI 10.1091/mbc.e04-05-0389, PubMed 15574884
Signaling, internalization, and intracellular activity of fibroblast growth factor
Curr Top Microbiol Immunol, 286, 45-79
DOI 10.1007/978-3-540-69494-6_3, PubMed 15645710
Design of fully active FGF-1 variants with increased stability
Protein Eng Des Sel, 17 (8), 603-11
DOI 10.1093/protein/gzh076, PubMed 15469994
Publications 2003
IL-7 receptor is present on human microvascular endothelial cells
Immunol Lett, 86 (2), 163-8
DOI 10.1016/s0165-2478(03)00018-x, PubMed 12644318
Translocation of FGF-1 and FGF-2 across vesicular membranes occurs during G1-phase by a common mechanism
Mol Biol Cell, 15 (2), 801-14
DOI 10.1091/mbc.e03-08-0589, PubMed 14657241
Phosphatidylinositol-3 kinase-dependent activation of Akt does not correlate with either high mitogenicity or cell migration induced by FGF-1
Anticancer Res, 23 (5A), 4071-7
PubMed 14666721
Transport of exogenous growth factors and cytokines to the cytosol and to the nucleus
Physiol Rev, 83 (1), 163-82
DOI 10.1152/physrev.00021.2002, PubMed 12506129
Publications 2002
Vesicle transmembrane potential is required for translocation to the cytosol of externally added FGF-1
EMBO J, 21 (17), 4480-90
DOI 10.1093/emboj/cdf472, PubMed 12198150
Steroid signal transduction activated at the cell membrane: from plants to animals
Acta Biochim Pol, 49 (3), 735-45
PubMed 12422243
Expression of human protein tyrosine phosphatase epsilon in leucocytes: a potential ERK pathway-regulating phosphatase
Scand J Immunol, 56 (2), 195-203
DOI 10.1046/j.1365-3083.2002.01126.x, PubMed 12121439
Publications 2001
Modulation of intracellular transport of acidic fibroblast growth factor by mutations in the cytoplasmic receptor domain
J Cell Sci, 114 (Pt 9), 1677-89
DOI 10.1242/jcs.114.9.1677, PubMed 11398757
Two farms of the heparin-binding EGF-like growth factor precursor, a receptor for diphtheria toxin
Mol. Biol., 35 (1), 122-129
DOI 10.1023/A:1004819321876
Evading apoptosis by calcitriol-differentiated human leukemic HL-60 cells is not mediated by changes in CD95 receptor system but by increased sensitivity of these cells to insulin
Exp Cell Res, 270 (1), 119-27
DOI 10.1006/excr.2001.5335, PubMed 11597134
Publications 2000
Requirement for C-terminal end of fibroblast growth factor receptor 4 in translocation of acidic fibroblast growth factor to cytosol and nucleus
J Cell Sci, 113 ( Pt 10), 1827-38
DOI 10.1242/jcs.113.10.1827, PubMed 10769213
Externally added aFGF mutants do not require extensive unfolding for transport to the cytosol and the nucleus in NIH/3T3 cells
Biochemistry, 39 (49), 15091-100
DOI 10.1021/bi001831k, PubMed 11106487
Publications 1999
Effects of mutations of a phosphorylation site in an exposed loop in acidic fibroblast growth factor
J Biol Chem, 274 (25), 18081-6
DOI 10.1074/jbc.274.25.18081, PubMed 10364261
[Activation of the cell proliferation program by acidic fibroblast growth factor (aFGF)]
Postepy Hig Med Dosw, 53 (2), 277-89
PubMed 10355293
Following angiogenin during angiogenesis: a journey from the cell surface to the nucleolus
Arch Immunol Ther Exp (Warsz), 47 (5), 299-305
PubMed 10604235
Publications 1998
Cloning of an intracellular protein that binds selectively to mitogenic acidic fibroblast growth factor
Biochem J, 336 ( Pt 1) (Pt 1), 213-22
DOI 10.1042/bj3360213, PubMed 9806903
Variation in the proregion structure of heparin-binding EGF-like growth factor precursors
Gene, 212 (1), 1-4
DOI 10.1016/s0378-1119(98)00139-5, PubMed 9661657
Evidence that phosphatidylinositol 3-kinase and p70S6K protein are involved in differentiation of HL-60 cells induced by calcitriol
Anticancer Res, 18 (5A), 3507-14
PubMed 9858932
Novel protein toxin-based strategy for development of cytotoxic T lymphocyte vaccines
Arch Immunol Ther Exp (Warsz), 46 (6), 341-6
PubMed 9883312
Modulation by interleukin-2 of cellular response to fibroblast growth factor-1 in F69-3 fibrosarcoma cells
Exp Cell Res, 244 (1), 61-70
DOI 10.1006/excr.1998.4187, PubMed 9770349
Publications 1997
Expression of mRNA for a short form of heparin-binding EGF-like growth factor
Gene, 195 (1), 81-6
DOI 10.1016/s0378-1119(97)00161-3, PubMed 9300824
1,25-Dihydroxyvitamin D3 induced activation and subsequent nuclear translocation of MAPK is upstream regulated by PKC in HL-60 cells
Biochem Biophys Res Commun, 241 (2), 419-26
DOI 10.1006/bbrc.1997.7832, PubMed 9425286
Effect of mutation of cytoplasmic receptor domain and of genistein on transport of acidic fibroblast growth factor into cells
Oncogene, 15 (5), 525-36
DOI 10.1038/sj.onc.1201226, PubMed 9247306
Diphtheria toxin as a molecular tool in the study of acidic fibroblast growth factor signalling
Arch Immunol Ther Exp (Warsz), 45 (5-6), 391-409
PubMed 9437495
Publications 1996
Retrograde transport of acidic fibroblast growth factor as a part of the growth factor signaling
Arch Immunol Ther Exp (Warsz), 44 (4), 201-7
PubMed 9017159
Stimulation of proliferation of a human osteosarcoma cell line by exogenous acidic fibroblast growth factor requires both activation of receptor tyrosine kinase and growth factor internalization
Mol Cell Biol, 16 (1), 270-80
DOI 10.1128/MCB.16.1.270, PubMed 8524304
Publications 1995
Farnesylation of CaaX-tagged diphtheria toxin A-fragment as a measure of transfer to the cytosol
Biochemistry, 34 (35), 11152-9
DOI 10.1021/bi00035a021, PubMed 7669773
Translocation of cytosol of exogenous, CAAX-tagged acidic fibroblast growth factor
J Biol Chem, 270 (51), 30680-5
DOI 10.1074/jbc.270.51.30680, PubMed 8530506
Publications 1994
Intermediates in translocation of diphtheria toxin across the plasma membrane
J Biol Chem, 269 (6), 4648-52
PubMed 7508447
Dual mode of signal transduction by externally added acidic fibroblast growth factor
Cell, 76 (6), 1039-51
DOI 10.1016/0092-8674(94)90381-6, PubMed 7511061
Publications 1992
Comparative studies on internalization of gold labelled mistletoe lectin I (MLI), its subunits, and of an immunotoxin into mouse L 1210V leukemia cells
Acta Histochem, 92 (1), 46-53
DOI 10.1016/S0065-1281(11)80140-6, PubMed 1580141
The activity of two immunotoxins composed of monoclonal antibody MoAb-16 and A-chain of ricin (MoAb-16-RTA) or A-chain of mistletoe lectin I (MoAb-16-MLIA)
Arch Immunol Ther Exp (Warsz), 40 (3-4), 223-7
PubMed 1300987
Tight folding of acidic fibroblast growth factor prevents its translocation to the cytosol with diphtheria toxin as vector
EMBO J, 11 (13), 4835-42
DOI 10.1002/j.1460-2075.1992.tb05589.x, PubMed 1281450
Publications 1991
Immunomagnetic removal of L1210V leukemic cells from mouse bone marrow ex vivo
Arch Immunol Ther Exp (Warsz), 39 (3), 235-41
PubMed 1810216
Internalization and action of an immunotoxin containing mistletoe lectin A-chain
Cancer Res, 51 (3), 916-20
PubMed 1988131
Publications 1990
Mouse L1210V leukemia as a model to analyse efficiency of leukemic cell elimination by immunotoxin, antibody and complement, and cytostatic agents
In Vivo, 4 (3), 209-13
PubMed 2133264
Publications 1989
Monoclonal antibodies against human haptoglobin
Hybridoma, 8 (5), 551-60
DOI 10.1089/hyb.1989.8.551, PubMed 2478448
The Thomsen-Friedenreich antigen on human urothelial cell lines detectable by peanut lectin and monoclonal antibody raised against human glycophorin A
Anticancer Res, 9 (1), 103-7
PubMed 2650614
Specific killing of mouse leukemic cells with ricin A-chain immunotoxin
Arch Immunol Ther Exp (Warsz), 37 (1-2), 101-13
PubMed 2619494
Publications 1988
Degradation of the human erythrocyte membrane band 3 studied with monoclonal antibody directed against an epitope on the cytoplasmic fragment of band 3
Eur J Biochem, 174 (4), 647-54
DOI 10.1111/j.1432-1033.1988.tb14147.x, PubMed 2455637
Two monoclonal antibodies recognizing different epitopes of blood group N antigen
Arch Immunol Ther Exp (Warsz), 36 (5), 623-32
PubMed 2478103