Deepak B. Thimiri Govinda Raj

  • Postdoc PhD
  • +47 2278 1752

Related imageRoom: K04.061


Drug Sensitivity Screening Platform:

Chronic Lymphocytic Leukemia (CLL) and Multiple Myeloma (MM) are considered incurable. Current challenges include design of optimal treatment for individual patients based on characterization of the tumor and its intratumor heterogeneity. Efficient therapies require a personalized approach that combines targeting both malignant B cells as well as its tumor microenvironment. I established invitro culture-setting for CLL and MM at Prof. Kjetil Tasken and Prof. Ludvig A Munthe’s group. To define drugs that inhibit malignant B cell growth, I perform drug sensitivity screening (DSS) on CLL (516 drugs) and MM (30 drugs). I select potential drug candidates along with pathway inhibitors and analyze by flow cytometry to assess effects on intracellular signaling. I propose to use the DSS platform to validate drug candidates for xenografts and precision medicine.

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Publications 2019

Thimiri Govinda Raj DB, Khan NA, Venkatachalam S, Chu DT (2019)
Step-by-Step Protocol for Superparamagnetic Nanoparticle-Based Plasma Membrane Isolation from Eukaryotic Cell
Methods Mol Biol (in press)
DOI 10.1007/7651_2019_211, PubMed 30771189

Thimiri Govinda Raj DB, Khan NA, Venkatachalam S, Chu DT, Arumugam S (2019)
Step-by-Step Protocol for Superparamagnetic Nanoparticle-Based Endosome and Lysosome Isolation from Eukaryotic Cell
Methods Mol Biol (in press)
DOI 10.1007/7651_2019_212, PubMed 30771190

Thimiri Govinda Raj DB, Niamat Ali Khan (2019)
Protocol for eukaryotic plasma membrane isolation using superparamagnetic nanoparticles
Journal of Magnetism and Magnetic materials, 476, 628-631
DOI 10.1016/j.jmmm.2017.12.070, PublikaID 300

Publications 2018

Thimiri Govinda Raj DB, Cremaschi A, Skånland SS, Gade A, Schjesvold FH, Tjønnfjord GE, Munthe LA, Tasken K (2018)
In-Vitro Drug Sensitivity Screening in Chronic Lymphocytic Leukemia (CLL) Primary Patient Samples Identifies Drug Candidates for Precision Cancer Therapy
Blood, 132, 4676
DOI 10.1182/blood-2018-99-110357, PublikaID 304

Thimiri Govinda Raj DB, Giliberto M, Cremaschi A, Skånland SS, Gade A, Tjønnfjord GE, Schjesvold FH, Munthe LA, Tasken K (2018)
Drug Sensitivity Screening on Multiple Myeloma for Precision Cancer Therapy
Blood, 132, 4677
DOI 10.1182/blood-2018-99-110669, PublikaID 303

Thimiri Govinda Raj DB, Khan NA (2018)
Surface functionalization dependent subcellular localization of Superparamagnetic nanoparticle in plasma membrane and endosome
Nano Converg, 5 (1), 4
DOI 10.1186/s40580-018-0136-3, PubMed 29492374

Thimiri Govinda Raj DB, Khan NA (2018)
Synthesis of hybrid AuNPs functionalised superparamagnetic NPs
IET Micro & Nano Letters, vol. 13, ( no. 3,), 292-296,
DOI 10.1049/mnl.2017.0574, PublikaID 301

Thimiri Govinda Raj DB, Subramaniam P, Venkatachalam SS, Chu DT (2018)
Targeted technology
EBR - European Biopharmaceutical Review, 2018 (October), pp. 70-71, 3, pp. 70-71.
PublikaID 299

Publications 2016

Khan NA, Willemarck N, Talebi A, Marchand A, Binda MM, Dehairs J, Rueda-Rincon N, Daniels VW, Bagadi M, Thimiri Govinda Raj DB, Vanderhoydonc F, Munck S, Chaltin P, Swinnen JV (2016)
Identification of drugs that restore primary cilium expression in cancer cells
Oncotarget, 7 (9), 9975-92
DOI 10.18632/oncotarget.7198, PubMed 26862738

Sari D, Gupta K, Thimiri Govinda Raj DB, Aubert A, Drncová P, Garzoni F, Fitzgerald D, Berger I (2016)
The MultiBac Baculovirus/Insect Cell Expression Vector System for Producing Complex Protein Biologics
Adv Exp Med Biol, 896, 199-215
DOI 10.1007/978-3-319-27216-0_13, PubMed 27165327

Thimiri Govinda Raj DB, Khan NA (2016)
Designer nanoparticle: nanobiotechnology tool for cell biology
Nano Converg, 3 (1), 22
DOI 10.1186/s40580-016-0082-x, PubMed 28191432

Publications 2014

Thimiri Govinda Raj DB, Vijayachandran LS, Berger I (2014)
OmniBac: universal multigene transfer plasmids for baculovirus expression vector systems
Methods Mol Biol, 1091, 123-30
DOI 10.1007/978-1-62703-691-7_7, PubMed 24203327

Publications 2013

Imre Berger, Thimiri Govinda Raj DB (2013)
European Patent, 2954, 050
PublikaID 307

Thimiri Govinda Raj DB, Annaert Wim, Borghs G (2013)
Biarsenical nanoparticles for in vivo labeling
European Patent WO, 2013076, 159A1
PublikaID 308

Vijayachandran LS, Thimiri Govinda Raj DB, Edelweiss E, Gupta K, Maier J, Gordeliy V, Fitzgerald DJ, Berger I (2013)
Gene gymnastics: Synthetic biology for baculovirus expression vector system engineering
Bioengineered, 4 (5), 279-87
DOI 10.4161/bioe.22966, PubMed 23328086

Publications 2011

Thimiri Govinda Raj DB, Ghesquière B, Tharkeshwar AK, Coen K, Derua R, Vanderschaeghe D, Rysman E, Bagadi M, Baatsen P, De Strooper B, Waelkens E, Borghs G, Callewaert N, Swinnen J, Gevaert K, Annaert W (2011)
A novel strategy for the comprehensive analysis of the biomolecular composition of isolated plasma membranes
Mol Syst Biol, 7, 541
DOI 10.1038/msb.2011.74, PubMed 22027552

Publications 2010

Gupta SC, Mishra M, Sharma A, Deepak Balaji TG, Kumar R, Mishra RK, Chowdhuri DK (2010)
Chlorpyrifos induces apoptosis and DNA damage in Drosophila through generation of reactive oxygen species
Ecotoxicol Environ Saf, 73 (6), 1415-23
DOI 10.1016/j.ecoenv.2010.05.013, PubMed 20627310

Thimiri Govinda Raj DB, Lagae L, Annaert W, Borghs G (2010)
Plasma membrane isolation
European Patent, EP, 2388589
PublikaID 306

Publications 2005

Thimiri Govinda Raj DB, Suresh KD, Prasanna SR, Murugasen M (2005)
Design of mixed microbial biosensor using T. cutaneum and K. marxianus culture for environmental monitoring.
IUP Journal of Science & Technology, 1 (3), 84-94
PublikaID 302

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