Per O. Seglen

  • Professor; PhD

Project description

Regulation of autophagic protein degradation, cytoskeletal organization and apoptotic cell death in normal and malignant rat liver cells

Normal tissues are continuously maintained and renewed by a balance between mass growth and autophagic degradation at the cellular level, and between cell division and apoptotic cell death at the tissue level. The uncontrolled growth of malignant tumours reflects the propensity of cancer cells to up-regulate mass growth and cell division as well as to down-regulate autophagy and apoptosis. Our project studies the molecular mechanisms involved in the regulation of the latter two processes, attempting to identify features that distinguish malignant cells from their normal counterparts.

Autophagy is a process by which cells, under conditions of stress or starvation, digest their own cytoplasm to obtain small molecules (like amino acids) needed for maintenance and renewal of essential cell functions. Specialized intracellular membrane cisternae, called phagophores, excise and enwrap pieces of cytoplasm, forming cytoplasm-filled vacuoles called autophagosomes. The autophagosomes subsequently fuse with endosomes (vacuoles that contain endocytosed material of extracellular origin) to form amphisomes, which eventually deliver their contents of cytoplasm and exogenous material to lysosomes (the digestive vacuoles of the cell) for degradation.

Our project has developed a method for purification of autophagosomes from isolated rat liver cells (Strømhaug et al. 1998, Biochem. J. 335, 217-224), and characterized these organelles biochemically as well as structurally (Fengsrud et al. 2000, Eur. J. Cell Biol. 79, 871-882). By the use of proteomics (protein separation by two-dimensional gel electrophoresis and column chromatography, combined with protein identification by mass spectrometry), we have been able to identify about 40 proteins that are selectively associated with autophagosomal membranes, including many novel enzyme forms (Fengsrud et al. 2000, Biochem. J. 352, 773-781). Several of these enzymes are functionally related, suggesting the participation of specific biochemical processes in the regulation of autophagic activity.

The use of proteomic methods has, furtermore, enabled our project to identify a signalling pathway that shuts down autophagy in liver cells during toxic stress. This pathway, which includes the AMP-activated protein kinase (AMPK), the stress-activated kinases SEK1 and JNK, and possibly S6-kinase (Ruud Larsen et al. 2002, J.Biol.Chem. 277, 34826-34835), also appears to regulate cytoskeletal organization and apoptotic cell death. Algal toxins like okadaic acid and microcystin induce a rapid activating phosphorylation of AMPK, and of cytoskeletal proteins like plectin and keratin, along with a disruption of the intracellular network of keratin intermediate filaments. This collapse of an important cytoskeletal element could well be a common cause of both the suppression of autophagy and of the apoptotic cell death that ensues several hours later.

A grapefuit flavonoid, naringin, prevents the AMPK phosphorylation, the keratin cytoskeletal collapse, the autophagy suppression and the apoptotic death elicited by algal toxins, probably reflecting an inhibitory effect of the flavonoid on an AMPK-activating protein kinase. Interestingly, naringin does not exert any such protective effect on liver cancer (hepatoma) cells of rat or human origin (Blankson et al. 2000, Cell Death Differ. 7, 739-746). The latter observation suggests the possibility of developing a novel type of cancer therapy, based on the use of a nonspecific cytotoxin (such as, e.g., okadaic acid) in combination with a specific cytoprotectant of normal cells (such as, e.g., naringin) to achieve a selective killing of cancer cells.

 

Publications 2021

Klionsky DJ, Abdel-Aziz AK, Abdelfatah S, Abdellatif M, Abdoli A, Abel S, Abeliovich H, Abildgaard MH, Abudu YP, Acevedo-Arozena A, Adamopoulos IE, Adeli K, Adolph TE, Adornetto A, Aflaki E, Agam G, Agarwal A, Aggarwal BB, Agnello M, Agostinis P, Agrewala JN, Agrotis A, Aguilar PV, Ahmad ST, Ahmed ZM et al. (2021)
Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)1
Autophagy, 17 (1), 1-382
DOI 10.1080/15548627.2020.1797280, PubMed 33634751

Publications 2019

Engedal N, Luhr M, Szalai P, Seglen PO (2019)
Measurement of Bulk Autophagy by a Cargo Sequestration Assay
Methods Mol Biol, 1880, 307-313
DOI 10.1007/978-1-4939-8873-0_20, PubMed 30610706

Publications 2016

Klionsky DJ, Abdelmohsen K, Abe A, Abedin MJ, Abeliovich H, Acevedo Arozena A, Adachi H, Adams CM, Adams PD, Adeli K, Adhihetty PJ, Adler SG, Agam G, Agarwal R, Aghi MK, Agnello M, Agostinis P, Aguilar PV, Aguirre-Ghiso J, Airoldi EM, Ait-Si-Ali S, Akematsu T, Akporiaye ET, Al-Rubeai M, Albaiceta GM et al. (2016)
Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)
Autophagy, 12 (1), 1-222
DOI 10.1080/15548627.2015.1100356, PubMed 26799652

Luhr M, Szalai P, Sætre F, Gerner L, Seglen PO, Engedal N (2016)
A Simple Cargo Sequestration Assay for Quantitative Measurement of Nonselective Autophagy in Cultured Cells
Methods Enzymol, 587, 351-364
DOI 10.1016/bs.mie.2016.09.064, PubMed 28253965

Publications 2015

Engedal N, Seglen PO (2015)
Autophagy of cytoplasmic bulk cargo does not require LC3
Autophagy, 12 (2), 439-41
DOI 10.1080/15548627.2015.1076606, PubMed 26237084

Saetre F, Hagen LK, Engedal N, Seglen PO (2015)
Novel steps in the autophagic-lysosomal pathway
FEBS J, 282 (11), 2202-14
DOI 10.1111/febs.13268, PubMed 25779646

Seglen PO, Luhr M, Mills IG, Sætre F, Szalai P, Engedal N (2015)
Macroautophagic cargo sequestration assays
Methods, 75, 25-36
DOI 10.1016/j.ymeth.2014.12.021, PubMed 25576638

Szalai P, Hagen LK, Sætre F, Luhr M, Sponheim M, Øverbye A, Mills IG, Seglen PO, Engedal N (2015)
Autophagic bulk sequestration of cytosolic cargo is independent of LC3, but requires GABARAPs
Exp Cell Res, 333 (1), 21-38
DOI 10.1016/j.yexcr.2015.02.003, PubMed 25684710

Publications 2013

Engedal N, Torgersen ML, Guldvik IJ, Barfeld SJ, Bakula D, Sætre F, Hagen LK, Patterson JB, Proikas-Cezanne T, Seglen PO, Simonsen A, Mills IG (2013)
Modulation of intracellular calcium homeostasis blocks autophagosome formation
Autophagy, 9 (10), 1475-90
DOI 10.4161/auto.25900, PubMed 23970164

Patel KK, Miyoshi H, Beatty WL, Head RD, Malvin NP, Cadwell K, Guan JL, Saitoh T, Akira S, Seglen PO, Dinauer MC, Virgin HW, Stappenbeck TS (2013)
Autophagy proteins control goblet cell function by potentiating reactive oxygen species production
EMBO J, 32 (24), 3130-44
DOI 10.1038/emboj.2013.233, PubMed 24185898

Publications 2012

Klionsky DJ, Abdalla FC, Abeliovich H, Abraham RT, Acevedo-Arozena A, Adeli K, Agholme L, Agnello M, Agostinis P, Aguirre-Ghiso JA, Ahn HJ, Ait-Mohamed O, Ait-Si-Ali S, Akematsu T, Akira S, Al-Younes HM, Al-Zeer MA, Albert ML, Albin RL, Alegre-Abarrategui J, Aleo MF, Alirezaei M, Almasan A, Almonte-Becerril M, Amano A et al. (2012)
Guidelines for the use and interpretation of assays for monitoring autophagy
Autophagy, 8 (4), 445-544
DOI 10.4161/auto.19496, PubMed 22966490

Publications 2011

Eskelinen EL, Reggiori F, Baba M, Kovács AL, Seglen PO (2011)
Seeing is believing: the impact of electron microscopy on autophagy research
Autophagy, 7 (9), 935-56
DOI 10.4161/auto.7.9.15760, PubMed 21566462

Øverbye A, Sætre F, Hagen LK, Johansen HT, Seglen PO (2011)
Autophagic activity measured in whole rat hepatocytes as the accumulation of a novel BHMT fragment (p10), generated in amphisomes by the asparaginyl proteinase, legumain
Autophagy, 7 (9), 1011-27
DOI 10.4161/auto.7.9.16436, PubMed 21610319

Publications 2010

Klionsky DJ, Seglen PO (2010)
The Norse god of autophagy. Interviewed by Daniel J Klionsky
Autophagy, 6 (8), 1017-31
DOI 10.4161/auto.6.8.13092, PubMed 20814236

Publications 2009

Seglen PO, Øverbye A, Saetre F (2009)
Sequestration assays for mammalian autophagy
Methods Enzymol, 452, 63-83
DOI 10.1016/S0076-6879(08)03605-7, PubMed 19200876

Øverbye A, Seglen PO (2009)
Phosphorylated and non-phosphorylated forms of catechol O-methyltransferase in rat liver, brain and other tissues
Biochem J, 417 (2), 535-45
DOI 10.1042/BJ20081284, PubMed 18831714

Publications 2008

Klionsky DJ, Elazar Z, Seglen PO, Rubinsztein DC (2008)
Does bafilomycin A1 block the fusion of autophagosomes with lysosomes?
Autophagy, 4 (7), 849-50
DOI 10.4161/auto.6845, PubMed 18758232

Seglen PO (2008)
Where endocytosis and autophagy meet: The amphisome
In Transworld Research Network (Fønhus MS, Mousavi SA, Berg T, eds.), Transworld Research Network, Trivandrum, India, 37/661, 61-100
PublikaID 37, ISBN 978-81-7895-364-9

Publications 2007

Klionsky DJ, Abeliovich H, Agostinis P, Agrawal DK, Aliev G, Askew DS, Baba M, Baehrecke EH, Bahr BA, Ballabio A, Bamber BA, Bassham DC, Bergamini E, Bi X, Biard-Piechaczyk M, Blum JS, Bredesen DE, Brodsky JL, Brumell JH, Brunk UT, Bursch W, Camougrand N, Cebollero E, Cecconi F, Chen Y et al. (2007)
Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes
Autophagy, 4 (2), 151-75
DOI 10.4161/auto.5338, PubMed 18188003

Klionsky DJ, Cuervo AM, Dunn WA, Levine B, van der Klei I, Seglen PO (2007)
How shall I eat thee?
Autophagy, 3 (5), 413-6
DOI 10.4161/auto.4377, PubMed 17568180

Klionsky DJ, Cuervo AM, Seglen PO (2007)
Methods for monitoring autophagy from yeast to human
Autophagy, 3 (3), 181-206
DOI 10.4161/auto.3678, PubMed 17224625

Øverbye A, Fengsrud M, Seglen PO (2007)
Proteomic analysis of membrane-associated proteins from rat liver autophagosomes
Autophagy, 3 (4), 300-22
DOI 10.4161/auto.3910, PubMed 17377489

Publications 2006

Seglen PO (2006)
Foreword
In Autophagy in Immunity and Infection (Deretic V, ed.), Wiley-VCH, Weinheim, XV-XVIII
PublikaID 38, ISBN 3-527-31450-4

Publications 2005

Lindstad RI, Sylte I, Mikalsen SO, Seglen PO, Berg E, Winberg JO (2005)
Pancreatic trypsin activates human promatrix metalloproteinase-2
J Mol Biol, 350 (4), 682-98
DOI 10.1016/j.jmb.2005.05.018, PubMed 15950241

Samari HR, Møller MT, Holden L, Asmyhr T, Seglen PO (2005)
Stimulation of hepatocytic AMP-activated protein kinase by okadaic acid and other autophagy-suppressive toxins
Biochem J, 386 (Pt 2), 237-44
DOI 10.1042/BJ20040609, PubMed 15461583

Sneve ML, Øverbye A, Fengsrud M, Seglen PO (2005)
Comigration of two autophagosome-associated dehydrogenases on two-dimensional polyacrylamide gels
Autophagy, 1 (3), 157-62
DOI 10.4161/auto.1.3.2037, PubMed 16874067

Publications 2004

Møller MT, Samari HR, Seglen PO (2004)
Toxin-induced tail phosphorylation of hepatocellular S6 kinase: evidence for a dual involvement of the AMP-activated protein kinase in S6 kinase regulation
Toxicol Sci, 82 (2), 628-37
DOI 10.1093/toxsci/kfh273, PubMed 15342961

Rusten TE, Lindmo K, Juhász G, Sass M, Seglen PO, Brech A, Stenmark H (2004)
Programmed autophagy in the Drosophila fat body is induced by ecdysone through regulation of the PI3K pathway
Dev Cell, 7 (2), 179-92
DOI 10.1016/j.devcel.2004.07.005, PubMed 15296715

Publications 2003

Møller MT, Samari HR, Fengsrud M, Strømhaug PE, øStvold AC, Seglen PO (2003)
Okadaic acid-induced, naringin-sensitive phosphorylation of glycine N-methyltransferase in isolated rat hepatocytes
Biochem J, 373 (Pt 2), 505-13
DOI 10.1042/BJ20030502, PubMed 12697024

Publications 2002

Larsen AK, Møller MT, Blankson H, Samari HR, Holden L, Seglen PO (2002)
Naringin-sensitive phosphorylation of plectin, a cytoskeletal cross-linking protein, in isolated rat hepatocytes
J Biol Chem, 277 (38), 34826-35
DOI 10.1074/jbc.M205028200, PubMed 12095991

Publications 2001

Berven G, Saetre F, Halvorsen K, Seglen PO (2001)
Effects of the diarrhetic shellfish toxin, okadaic acid, on cytoskeletal elements, viability and functionality of rat liver and intestinal cells
Toxicon, 39 (2-3), 349-62
DOI 10.1016/s0041-0101(00)00137-9, PubMed 10978754

Mousavi SA, Kjeken R, Berg TO, Seglen PO, Berg T, Brech A (2001)
Effects of inhibitors of the vacuolar proton pump on hepatic heterophagy and autophagy
Biochim Biophys Acta, 1510 (1-2), 243-57
DOI 10.1016/s0005-2736(00)00354-0, PubMed 11342162

Seglen PO (2001)
Evaluating biology: a scientometric study of a university biology department
In NIFU skriftserie, NIFU, Oslo, nr 6/2001, 70 s.
BIBSYS 010899006

Publications 2000

Blankson H, Grotterød EM, Seglen PO (2000)
Prevention of toxin-induced cytoskeletal disruption and apoptotic liver cell death by the grapefruit flavonoid, naringin
Cell Death Differ, 7 (8), 739-46
DOI 10.1038/sj.cdd.4400705, PubMed 10918448

Fengsrud M, Erichsen ES, Berg TO, Raiborg C, Seglen PO (2000)
Ultrastructural characterization of the delimiting membranes of isolated autophagosomes and amphisomes by freeze-fracture electron microscopy
Eur J Cell Biol, 79 (12), 871-82
DOI 10.1078/0171-9335-00125, PubMed 11152279

Fengsrud M, Raiborg C, Berg TO, Strømhaug PE, Ueno T, Erichsen ES, Seglen PO (2000)
Autophagosome-associated variant isoforms of cytosolic enzymes
Biochem J, 352 Pt 3 (Pt 3), 773-81
DOI 10.1042/0264-6021:3520773, PubMed 11104685

Publications 1998

Berg TO, Fengsrud M, Strømhaug PE, Berg T, Seglen PO (1998)
Isolation and characterization of rat liver amphisomes. Evidence for fusion of autophagosomes with both early and late endosomes
J Biol Chem, 273 (34), 21883-92
DOI 10.1074/jbc.273.34.21883, PubMed 9705327

Kovács AL, Gordon PB, Grotterød EM, Seglen PO (1998)
Inhibition of hepatocytic autophagy by adenosine, adenosine analogs and AMP
Biol Chem, 379 (11), 1341-7
DOI 10.1515/bchm.1998.379.11.1341, PubMed 9865607

Rundén E, Seglen PO, Haug FM, Ottersen OP, Wieloch T, Shamloo M, Laake JH (1998)
Regional selective neuronal degeneration after protein phosphatase inhibition in hippocampal slice cultures: evidence for a MAP kinase-dependent mechanism
J Neurosci, 18 (18), 7296-305
DOI 10.1523/JNEUROSCI.18-18-07296.1998, PubMed 9736650

Samari HR, Seglen PO (1998)
Inhibition of hepatocytic autophagy by adenosine, aminoimidazole-4-carboxamide riboside, and N6-mercaptopurine riboside. Evidence for involvement of amp-activated protein kinase
J Biol Chem, 273 (37), 23758-63
DOI 10.1074/jbc.273.37.23758, PubMed 9726984

Seglen PO (1998)
Citation rates and journal impact factors are not suitable for evaluation of research
Acta Orthop Scand, 69 (3), 224-9
DOI 10.3109/17453679809000920, PubMed 9703393

Strømhaug PE, Berg TO, Fengsrud M, Seglen PO (1998)
Purification and characterization of autophagosomes from rat hepatocytes
Biochem J, 335 ( Pt 2) (Pt 2), 217-24
DOI 10.1042/bj3350217, PubMed 9761717

Publications 1997

Seglen PO (1997)
Why the impact factor of journals should not be used for evaluating research
BMJ, 314 (7079), 498-502
DOI 10.1136/bmj.314.7079.497, PubMed 9056804

Seglen PO (1997)
DNA ploidy and autophagic protein degradation as determinants of hepatocellular growth and survival
Cell Biol Toxicol, 13 (4-5), 301-15
DOI 10.1023/a:1007487425047, PubMed 9298250

Seglen PO (1997)
Citations and journal impact factors: questionable indicators of research quality
Allergy, 52 (11), 1050-6
DOI 10.1111/j.1398-9995.1997.tb00175.x, PubMed 9404555

Strømhaug PE, Berg TO, Berg K, Seglen PO (1997)
A novel method for the study of autophagy: destruction of hepatocytic lysosomes, but not autophagosomes, by the photosensitizing porphyrin tetra(4-sulphonatophenyl)porphine
Biochem J, 321 ( Pt 1) (Pt 1), 217-25
DOI 10.1042/bj3210217, PubMed 9003422

Strømhaug PE, Berg TO, Gjøen T, Seglen PO (1997)
Differences between fluid-phase endocytosis (pinocytosis) and receptor-mediated endocytosis in isolated rat hepatocytes
Eur J Cell Biol, 73 (1), 28-39
PubMed 9174669

Publications 1996

Holen I, Gordon PB, Strømhaug PE, Seglen PO (1996)
Role of cAMP in the regulation of hepatocytic autophagy
Eur J Biochem, 236 (1), 163-70
DOI 10.1111/j.1432-1033.1996.00163.x, PubMed 8617261

Seglen PO, Berg TO, Blankson H, Fengsrud M, Holen I, Strømhaug PE (1996)
Structural aspects of autophagy
Adv Exp Med Biol, 389, 103-11
DOI 10.1007/978-1-4613-0335-0_12, PubMed 8860999

Publications 1995

Blankson H, Holen I, Seglen PO (1995)
Disruption of the cytokeratin cytoskeleton and inhibition of hepatocytic autophagy by okadaic acid
Exp Cell Res, 218 (2), 522-30
DOI 10.1006/excr.1995.1187, PubMed 7540986

Fengsrud M, Roos N, Berg T, Liou W, Slot JW, Seglen PO (1995)
Ultrastructural and immunocytochemical characterization of autophagic vacuoles in isolated hepatocytes: effects of vinblastine and asparagine on vacuole distributions
Exp Cell Res, 221 (2), 504-19
DOI 10.1006/excr.1995.1402, PubMed 7493651

Fosse M, Berg TO, O'Reilly DS, Seglen PO (1995)
Vanadate inhibition of hepatocytic autophagy. Calcium-modulated and osmolality-modulated antagonism by asparagine
Eur J Biochem, 230 (1), 17-24
DOI 10.1111/j.1432-1033.1995.0017i.x, PubMed 7601097

Gordon PB, Holen I, Seglen PO (1995)
Protection by naringin and some other flavonoids of hepatocytic autophagy and endocytosis against inhibition by okadaic acid
J Biol Chem, 270 (11), 5830-8
DOI 10.1074/jbc.270.11.5830, PubMed 7890712

Holen I, Gordon PB, Strømhaug PE, Berg TO, Fengsrud M, Brech A, Roos N, Berg T, Seglen PO (1995)
Inhibition of asialoglycoprotein endocytosis and degradation in rat hepatocytes by protein phosphatase inhibitors
Biochem J, 311 ( Pt 1) (Pt 1), 317-26
DOI 10.1042/bj3110317, PubMed 7575471

Holen I, Strømhaug PE, Gordon PB, Fengsrud M, Berg TO, Seglen PO (1995)
Inhibition of autophagy and multiple steps in asialoglycoprotein endocytosis by inhibitors of tyrosine protein kinases (tyrphostins)
J Biol Chem, 270 (21), 12823-31
DOI 10.1074/jbc.270.21.12823, PubMed 7759538

Publications 1994

Berg TO, Strømhaug PE, Berg T, Seglen PO (1994)
Separation of lysosomes and autophagosomes by means of glycyl-phenylalanine-naphthylamide, a lysosome-disrupting cathepsin-C substrate
Eur J Biochem, 221 (1), 595-602
DOI 10.1111/j.1432-1033.1994.tb18771.x, PubMed 8168547

Drettner B, Seglen PO, Sivertsen G (1994)
[The impact factor as an instrument of allocation. Not accepted by the journals in Scandinavia]
Lakartidningen, 91 (8), 744-5
PubMed 8189977

Gerlyng P, Grotmol T, Seglen PO (1994)
Effect of 4-acetylaminofluorene and other tumour promoters on hepatocellular growth and binucleation
Carcinogenesis, 15 (2), 371-9
DOI 10.1093/carcin/15.2.371, PubMed 7508825

Gerlyng P, Grotmol T, Stokke T, Erikstein B, Seglen PO (1994)
Flow cytometric investigation of a possible precursor--product relationship between oval cells and parenchymal cells in the rat liver
Carcinogenesis, 15 (1), 53-9
DOI 10.1093/carcin/15.1.53, PubMed 8293549

Jonassen TS, Seglen PO, Stokke T (1994)
The fraction of cells in G1 with bound retinoblastoma protein increases with the duration of the cell cycle
Cell Prolif, 27 (2), 95-104
DOI 10.1111/j.1365-2184.1994.tb01408.x, PubMed 10465029

Mossin L, Blankson H, Huitfeldt H, Seglen PO (1994)
Ploidy-dependent growth and binucleation in cultured rat hepatocytes
Exp Cell Res, 214 (2), 551-60
DOI 10.1006/excr.1994.1293, PubMed 7925649

Seglen P, Saeter G, Schwarze P (1994)
Proliferation of diploid hepatocytes and nonparenchymal (oval) cells during rat-liver regeneration in the presence of 2-acetylaminofluorene
Int J Oncol, 5 (4), 805-10
DOI 10.3892/ijo.5.4.805, PubMed 21559645

Seglen PO (1994)
[How should the research be directed?]
Tidsskr Nor Laegeforen, 114 (25), 2970-2
PubMed 7974410

Publications 1993

Gerlyng P, Abyholm A, Grotmol T, Erikstein B, Huitfeldt HS, Stokke T, Seglen PO (1993)
Binucleation and polyploidization patterns in developmental and regenerative rat liver growth
Cell Prolif, 26 (6), 557-65
DOI 10.1111/j.1365-2184.1993.tb00033.x, PubMed 9116122

Gordon PB, Holen I, Fosse M, Røtnes JS, Seglen PO (1993)
Dependence of hepatocytic autophagy on intracellularly sequestered calcium
J Biol Chem, 268 (35), 26107-12
PubMed 8253727

Holen I, Gordon PB, Seglen PO (1993)
Inhibition of hepatocytic autophagy by okadaic acid and other protein phosphatase inhibitors
Eur J Biochem, 215 (1), 113-22
DOI 10.1111/j.1432-1033.1993.tb18013.x, PubMed 8393787

Kisen GO, Tessitore L, Costelli P, Gordon PB, Schwarze PE, Baccino FM, Seglen PO (1993)
Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells
Carcinogenesis, 14 (12), 2501-5
DOI 10.1093/carcin/14.12.2501, PubMed 8269618

Strømhaug PE, Seglen PO (1993)
Evidence for acidity of prelysosomal autophagic/endocytic vacuoles (amphisomes)
Biochem J, 291 ( Pt 1) (Pt 1), 115-21
DOI 10.1042/bj2910115, PubMed 8471030

Publications 1992

Bohley P, Seglen PO (1992)
Proteases and proteolysis in the lysosome
Experientia, 48 (2), 151-7
DOI 10.1007/BF01923508, PubMed 1740187

Gerlyng P, Grotmol T, Erikstein B, Stokke T, Seglen PO (1992)
Reduced proliferative activity of polyploid cells in primary hepatocellular carcinoma
Carcinogenesis, 13 (10), 1795-801
DOI 10.1093/carcin/13.10.1795, PubMed 1330342

Gerlyng P, Stokke T, Huitfeldt HS, Stenersen T, Danielsen HE, Grotmol T, Seglen PO (1992)
Analytical methods for the study of liver cell proliferation
Cytometry, 13 (4), 404-15
DOI 10.1002/cyto.990130411, PubMed 1382009

Gordon PB, Høyvik H, Seglen PO (1992)
Prelysosomal and lysosomal connections between autophagy and endocytosis
Biochem J, 283 ( Pt 2) (Pt 2), 361-9
DOI 10.1042/bj2830361, PubMed 1575680

Holen I, Gordon PB, Seglen PO (1992)
Protein kinase-dependent effects of okadaic acid on hepatocytic autophagy and cytoskeletal integrity
Biochem J, 284 ( Pt 3) (Pt 3), 633-6
DOI 10.1042/bj2840633, PubMed 1320371

Seglen PO, Bohley P (1992)
Autophagy and other vacuolar protein degradation mechanisms
Experientia, 48 (2), 158-72
DOI 10.1007/BF01923509, PubMed 1740188

Publications 1991

Gordon PB, Holen I, Seglen PO (1991)
Effects of adrenergic agonists and antagonists on autophagic activity in isolated rat liver cells
Biomed Biochim Acta, 50 (4-6), 383-7
PubMed 1801702

Holen I, Gordon PB, Seglen PO (1991)
Role of cyclic nucleotides in the control of hepatic autophagy
Biomed Biochim Acta, 50 (4-6), 389-92
PubMed 1666282

Høyvik H, Gordon PB, Berg TO, Strømhaug PE, Seglen PO (1991)
Inhibition of autophagic-lysosomal delivery and autophagic lactolysis by asparagine
J Cell Biol, 113 (6), 1305-12
DOI 10.1083/jcb.113.6.1305, PubMed 1904444

Schwarze PE, Saeter G, Armstrong D, Cameron RG, Laconi E, Sarma DS, Préat V, Seglen PO (1991)
Diploid growth pattern of hepatocellular tumours induced by various carcinogenic treatments
Carcinogenesis, 12 (2), 325-7
DOI 10.1093/carcin/12.2.325, PubMed 1847321

Seglen PO (1991)
Citation frequency and journal impact: valid indicators of scientific quality?
J Intern Med, 229 (2), 109-11
DOI 10.1111/j.1365-2796.1991.tb00316.x, PubMed 1997634

Seglen PO, Gordon PB, Holen I, Høyvik H (1991)
Hepatocytic autophagy
Biomed Biochim Acta, 50 (4-6), 373-81
PubMed 1666281

Publications 1990

Gerlyng P, Seglen PO (1990)
Effects of the tumor promoter 2-acetylaminofluorene and of 4-acetylaminofluorene on rat liver growth
Bull Cancer, 77 (5), 461-6
PubMed 2400817

Hendil KB, Lauridsen AM, Seglen PO (1990)
Both endocytic and endogenous protein degradation in fibroblasts is stimulated by serum/amino acid deprivation and inhibited by 3-methyladenine
Biochem J, 272 (3), 577-81
DOI 10.1042/bj2720577, PubMed 1702625

Kopitz J, Kisen GO, Gordon PB, Bohley P, Seglen PO (1990)
Nonselective autophagy of cytosolic enzymes by isolated rat hepatocytes
J Cell Biol, 111 (3), 941-53
DOI 10.1083/jcb.111.3.941, PubMed 2391370

Pichiri-Coni G, Coni P, Laconi E, Schwarze PE, Seglen PO, Rao PM, Rajalakshmi S, Sarma DS (1990)
Studies on the mitoinhibitory effect of orotic acid on hepatocytes in primary culture
Carcinogenesis, 11 (6), 981-4
DOI 10.1093/carcin/11.6.981, PubMed 2347072

Saeter G, Seglen PO (1990)
Cell biology of hepatocarcinogenesis
Crit Rev Oncog, 1 (4), 437-66
PubMed 1964598

Seglen PO, Gerlyng P (1990)
Growth-related alterations during liver carcinogenesis: effect of promoters
Environ Health Perspect, 88, 197-205
DOI 10.1289/ehp.9088197, PubMed 2272315

Seglen PO, Gordon PB, Holen I (1990)
Non-selective autophagy
Semin Cell Biol, 1 (6), 441-8
PubMed 2103895

Seglen PO, Saeter G, Schwarze PE (1990)
Liver tumor promoters stimulate growth of transplanted hepatocellular carcinomas
Hepatology, 12 (2), 295-300
DOI 10.1002/hep.1840120217, PubMed 2167869

Publications 1989

Gordon PB, Kisen GO, Kovács AL, Seglen PO (1989)
Experimental characterization of the autophagic-lysosomal pathway in isolated rat hepatocytes
Biochem Soc Symp, 55, 129-43
PubMed 2619764

Plomp PJ, Gordon PB, Meijer AJ, Høyvik H, Seglen PO (1989)
Energy dependence of different steps in the autophagic-lysosomal pathway
J Biol Chem, 264 (12), 6699-704
PubMed 2708336

Saeter G, Schwarze PE, Nesland JM, Seglen PO (1989)
Diploid nature of hepatocellular tumours developing from transplanted preneoplastic liver cells
Br J Cancer, 59 (2), 198-205
DOI 10.1038/bjc.1989.41, PubMed 2930686

Seglen PO (1989)
[Evaluation of scientific quality using citation analysis and other bibliometric methods]
Nord Med, 104 (12), 331-5, 341
PubMed 2594499

Seglen PO (1989)
[Use of citation analysis and other bibliometric methods in evaluation of the quality of research]
Tidsskr Nor Laegeforen, 109 (31), 3229-4
PubMed 2595690

Seglen PO (1989)
From bad to worse: evaluation by Journal Impact
Trends Biochem Sci, 14 (8), 326-7
DOI 10.1016/0968-0004(89)90163-1, PubMed 2799903

Seglen PO, Skomedal H, Saeter G, Schwarze PE, Nesland JM (1989)
Neuroendocrine dysdifferentiation and bombesin production in carcinogen-induced hepatocellular rat tumours
Carcinogenesis, 10 (1), 21-5
DOI 10.1093/carcin/10.1.21, PubMed 2910524

Publications 1988

Gordon PB, Seglen PO (1988)
Prelysosomal convergence of autophagic and endocytic pathways
Biochem Biophys Res Commun, 151 (1), 40-7
DOI 10.1016/0006-291x(88)90556-6, PubMed 3126737

Saeter G, Lee CZ, Schwarze PE, Ous S, Chen DS, Sung JL, Seglen PO (1988)
Changes in ploidy distributions in human liver carcinogenesis
J Natl Cancer Inst, 80 (18), 1480-5
DOI 10.1093/jnci/80.18.1480, PubMed 2846856

Saeter G, Schwarze PE, Nesland JM, Juul N, Pettersen EO, Seglen PO (1988)
The polyploidizing growth pattern of normal rat liver is replaced by divisional, diploid growth in hepatocellular nodules and carcinomas
Carcinogenesis, 9 (6), 939-45
DOI 10.1093/carcin/9.6.939, PubMed 3370757

Saeter G, Schwarze PE, Nesland JM, Seglen PO (1988)
2-Acetylaminofluorene promotion of liver carcinogenesis by a non-cytotoxic mechanism
Carcinogenesis, 9 (4), 581-7
DOI 10.1093/carcin/9.4.581, PubMed 2895691

Publications 1987

Gordon PB, Høyvik H, Seglen PO (1987)
Sugar uptake by fluid-phase pinocytosis and diffusion in isolated rat hepatocytes
Biochem J, 243 (3), 655-60
DOI 10.1042/bj2430655, PubMed 3663095

Gordon PB, Kovacs AL, Seglen PO (1987)
Temperature dependence of protein degradation, autophagic sequestration and mitochondrial sugar uptake in rat hepatocytes
Biochim Biophys Acta, 929 (2), 128-33
DOI 10.1016/0167-4889(87)90167-4, PubMed 3593777

Plomp PJ, Wolvetang EJ, Groen AK, Meijer AJ, Gordon PB, Seglen PO (1987)
Energy dependence of autophagic protein degradation in isolated rat hepatocytes
Eur J Biochem, 164 (1), 197-203
DOI 10.1111/j.1432-1033.1987.tb11011.x, PubMed 3830181

Saeter G, Schwarze PE, Nesland JM, Seglen PO (1987)
Transplantation of preneoplastic rat hepatocytes by intraportal injection
Toxicol Pathol, 15 (1), 78-81
DOI 10.1177/019262338701500110, PubMed 3554470

Schwarze PE, Tolleshaug H, Seglen PO (1987)
Endocytosis of asialo-glycoproteins and attachment to asialo-glycoproteins of hepatocytes from carcinogen-treated rats
Toxicol Pathol, 15 (1), 88-92
DOI 10.1177/019262338701500112, PubMed 3576077

Publications 1986

Gordon PB, Seglen PO (1986)
Use of electrical methods in the study of hepatocytic autophagy
Biomed Biochim Acta, 45 (11-12), 1635-45
PubMed 3579882

Høyvik H, Gordon PB, Seglen PO (1986)
Use of a hydrolysable probe, [14C]lactose, to distinguish between pre-lysosomal and lysosomal steps in the autophagic pathway
Exp Cell Res, 166 (1), 1-14
DOI 10.1016/0014-4827(86)90503-3, PubMed 3743649

Melvik JE, Pettersen EO, Gordon PB, Seglen PO (1986)
Increase in cis-dichlorodiammineplatinum (II) cytotoxicity upon reversible electropermeabilization of the plasma membrane in cultured human NHIK 3025 cells
Eur J Cancer Clin Oncol, 22 (12), 1523-30
DOI 10.1016/0277-5379(86)90090-8, PubMed 3595677

Schwarze PE, Pettersen EO, Seglen PO (1986)
Characterization of hepatocytes from carcinogen-treated rats by two-parametric flow cytometry
Carcinogenesis, 7 (1), 171-3
DOI 10.1093/carcin/7.1.171, PubMed 3943139

Schwarze PE, Pettersen EO, Tolleshaug H, Seglen PO (1986)
Isolation of carcinogen-induced diploid rat hepatocytes by centrifugal elutriation
Cancer Res, 46 (9), 4732-7
PubMed 2873888

Seglen PO, Gordon PB, Høyvik H (1986)
Radiolabelled sugars as probes of hepatocytic autophagy
Biomed Biochim Acta, 45 (11-12), 1647-56
PubMed 3579883

Seglen PO, Gordon PB, Tolleshaug H, Høyvik H (1986)
Use of [3H]raffinose as a specific probe of autophagic sequestration
Exp Cell Res, 162 (1), 273-7
DOI 10.1016/0014-4827(86)90446-5, PubMed 3940229

Seglen PO, Schwarze PE, Saeter G (1986)
Changes in cellular ploidy and autophagic responsiveness during rat liver carcinogenesis
Toxicol Pathol, 14 (3), 342-8
DOI 10.1177/019262338601400309, PubMed 3787117

Publications 1985

Gordon PB, Høyvik H, Seglen PO (1985)
Sequestration and hydrolysis of electroinjected [14C]lactose as a means of investigating autophagosome-lysosome fusion in isolated rat hepatocytes
Prog Clin Biol Res, 180, 475-7
PubMed 4034554

Gordon PB, Tolleshaug H, Seglen PO (1985)
Autophagic sequestration of [14C]sucrose introduced into isolated rat hepatocytes by electrical and non-electrical methods
Exp Cell Res, 160 (2), 449-58
DOI 10.1016/0014-4827(85)90192-2, PubMed 4043252

Gordon PB, Tolleshaug H, Seglen PO (1985)
Use of digitonin extraction to distinguish between autophagic-lysosomal sequestration and mitochondrial uptake of [14C]sucrose in hepatocytes
Biochem J, 232 (3), 773-80
DOI 10.1042/bj2320773, PubMed 4091820

Rogne S, Rønning OW, Myklebost O, Seglen PO, Pettersen EO (1985)
Cell cycle traverse and protein metabolism in human NHIK 3025 cells: the role of anchorage
J Cell Physiol, 125 (3), 528-32
DOI 10.1002/jcp.1041250324, PubMed 4066771

Schwarze PE, Seglen PO (1985)
Reduced autophagic activity, improved protein balance and enhanced in vitro survival of hepatocytes isolated from carcinogen-treated rats
Exp Cell Res, 157 (1), 15-28
DOI 10.1016/0014-4827(85)90148-x, PubMed 2857648

Seglen PO, Gordon PB, Tolleshaug H, Høyvik H (1985)
Pathways of intracellular sequestration and protein degradation in isolated rat hepatocytes
Prog Clin Biol Res, 180, 437-46
PubMed 4034550

Seglen PO, Gordon PB, Tolleshaug H, Høyvik H (1985)
Autophagy and protein degradation in isolated rat hepatocytes
Biochem Soc Trans, 13 (6), 1007-10
DOI 10.1042/bst0131007, PubMed 4092819

Seglen PO, Solheim AE (1985)
Conversion of dense lysosomes into light lysosomes during hepatocytic autophagy
Exp Cell Res, 157 (2), 550-5
DOI 10.1016/0014-4827(85)90141-7, PubMed 3979450

Tolleshaug H, Seglen PO (1985)
Autophagic-lysosomal and mitochondrial sequestration of [14C]sucrose. Density gradient distribution of sequestered radioactivity
Eur J Biochem, 153 (2), 223-9
DOI 10.1111/j.1432-1033.1985.tb09290.x, PubMed 4076173

Publications 1984

Schwarze PE, Pettersen EO, Shoaib MC, Seglen PO (1984)
Emergence of a population of small, diploid hepatocytes during hepatocarcinogenesis
Carcinogenesis, 5 (10), 1267-75
DOI 10.1093/carcin/5.10.1267, PubMed 6149024

Seglen PO, Gordon PB (1984)
Amino acid control of autophagic sequestration and protein degradation in isolated rat hepatocytes
J Cell Biol, 99 (2), 435-44
DOI 10.1083/jcb.99.2.435, PubMed 6746735

Seglen PO, Munthe-Kaas AC, Dybedal MA (1984)
Amino acid control of protein degradation in normal and leukemic human lymphocytes
Exp Cell Res, 155 (1), 121-8
DOI 10.1016/0014-4827(84)90773-0, PubMed 6489454

Tolleshaug H, Gordon PB, Solheim AE, Seglen PO (1984)
Trapping of electro-injected [14C]sucrose by hepatocyte mitochondria: a mechanism for cellular autofiltration?
Biochem Biophys Res Commun, 119 (3), 955-61
DOI 10.1016/0006-291x(84)90866-0, PubMed 6712679

Publications 1983

Schwarze PE, Seglen PO (1983)
Paradoxical stimulation by amino acids of the degradation of [35S]methionine-labelled, short-lived protein in isolated rat hepatocytes
Biochem Biophys Res Commun, 117 (2), 509-16
DOI 10.1016/0006-291x(83)91229-9, PubMed 6661239

Seglen PO (1983)
Inhibitors of lysosomal function
Methods Enzymol, 96, 737-64
DOI 10.1016/s0076-6879(83)96063-9, PubMed 6361463

Solheim AE, Seglen PO (1983)
Structural and physical changes in lysosomes from isolated rat hepatocytes treated with methylamine
Biochim Biophys Acta, 763 (3), 284-91
DOI 10.1016/0167-4889(83)90136-2, PubMed 6626583

Solheim AE, Seglen PO (1983)
Cellular and lysosomal uptake of methylamine in isolated rat hepatocytes
Biochem J, 210 (3), 929-36
DOI 10.1042/bj2100929, PubMed 6870811

Publications 1982

Gordon PB, Seglen PO (1982)
6-substituted purines: a novel class of inhibitors of endogenous protein degradation in isolated rat hepatocytes
Arch Biochem Biophys, 217 (1), 282-94
DOI 10.1016/0003-9861(82)90504-5, PubMed 6889839

Gordon PB, Seglen PO (1982)
Autophagic sequestration of [14C]sucrose, introduced into rat hepatocytes by reversible electro-permeabilization
Exp Cell Res, 142 (1), 1-14
DOI 10.1016/0014-4827(82)90402-5, PubMed 7140848

Kovács AL, Reith A, Seglen PO (1982)
Accumulation of autophagosomes after inhibition of hepatocytic protein degradation by vinblastine, leupeptin or a lysosomotropic amine
Exp Cell Res, 137 (1), 191-201
DOI 10.1016/0014-4827(82)90020-9, PubMed 7056284

Kovács AL, Seglen PO (1982)
Inhibition of hepatocytic protein degradation by inducers of autophagosome accumulation
Acta Biol Med Ger, 41 (1), 125-30
PubMed 7113543

Rønning OW, Seglen PO (1982)
The relation between protein accumulation and cell cycle traverse of human NHIK 3025 cells in unbalanced growth
J Cell Physiol, 112 (1), 19-26
DOI 10.1002/jcp.1041120105, PubMed 7107714

Schwarze PE, Solheim AE, Seglen PO (1982)
Amino acid and energy requirements for rat hepatocytes in primary culture
In Vitro, 18 (1), 43-54
DOI 10.1007/BF02796384, PubMed 6763597

Seglen PO, Gordon PB (1982)
3-Methyladenine: specific inhibitor of autophagic/lysosomal protein degradation in isolated rat hepatocytes
Proc Natl Acad Sci U S A, 79 (6), 1889-92
DOI 10.1073/pnas.79.6.1889, PubMed 6952238

Publications 1981

Grinde B, Seglen PO (1981)
Role of microtubuli in the lysosomal degradation of endogenous and exogenous protein in isolated rat hepatocytes
Hoppe Seylers Z Physiol Chem, 362 (5), 549-56
DOI 10.1515/bchm2.1981.362.1.549, PubMed 6166527

Grinde B, Seglen PO (1981)
Effects of amino acids and amino acid analogues on lysosomal protein degradation in isolated rat hepatocytes
Acta Biol Med Ger, 40 (10-11), 1603-12
PubMed 6177140

Grinde B, Seglen PO (1981)
Leucine inhibition of autophagic vacuole formation in isolated rat hepatocytes
Exp Cell Res, 134 (1), 33-9
DOI 10.1016/0014-4827(81)90460-2, PubMed 7250217

Grinde B, Seglen PO (1981)
Effects of amino acid analogues on protein degradation in isolated rat hepatocytes
Biochim Biophys Acta, 676 (1), 43-50
DOI 10.1016/0304-4165(81)90007-6, PubMed 7260111

Kovács AL, Grinde B, Seglen PO (1981)
Inhibition of autophagic vacuole formation and protein degradation by amino acids in isolated hepatocytes
Exp Cell Res, 133 (2), 431-6
DOI 10.1016/0014-4827(81)90336-0, PubMed 7238609

Kovács AL, Molnár K, Seglen PO (1981)
Inhibition of autophagic sequestration and endogenous protein degradation in isolated rat hepatocytes by methylated adenosine derivatives
FEBS Lett, 134 (2), 194-6
DOI 10.1016/0014-5793(81)80600-x, PubMed 7308488

Kovács AL, Seglen PO (1981)
Inhibition of hepatocytic protein degradation by methylaminopurines and inhibitors of protein synthesis
Biochim Biophys Acta, 676 (2), 213-20
DOI 10.1016/0304-4165(81)90189-6, PubMed 7260116

Poli A, Gordon PB, Schwarze PE, Grinde B, Seglen PO (1981)
Effects of insulin and anchorage on hepatocytic protein metabolism and amino acid transport
J Cell Sci, 48, 1-18
DOI 10.1242/jcs.48.1.1, PubMed 7024288

Rønning OW, Lindmo T, Pettersen EO, Seglen PO (1981)
Effect of serum step-down on protein metabolism and proliferation kinetics of NHIK 3025 cells
J Cell Physiol, 107 (1), 47-57
DOI 10.1002/jcp.1041070107, PubMed 7194351

Rønning OW, Lindmo T, Pettersen EO, Seglen PO (1981)
The role of protein accumulation in the cell cycle control of human NHIK 3025 cells
J Cell Physiol, 109 (3), 411-8
DOI 10.1002/jcp.1041090306, PubMed 7320058

Schwarze PE, Seglen PO (1981)
Effects of antibiotics on protein synthesis and degradation in primary cultures of rat hepatocytes
In Vitro, 17 (1), 71-6
DOI 10.1007/BF02618033, PubMed 7216241

Seglen PO, Gordon PB (1981)
Vanadate inhibits protein degradation in isolated rat hepatocytes
J Biol Chem, 256 (15), 7699-701
PubMed 6167567

Seglen PO, Gordon PB, Grinde B, Solheim A, Kovács AL, Poli A (1981)
Inhibitors and pathways of hepatocytic protein degradation
Acta Biol Med Ger, 40 (10-11), 1587-98
PubMed 7342604

Publications 1980

Gjessing R, Seglen PO (1980)
Adsorption, simple binding and complex binding of rat hepatocytes to various in vitro substrata
Exp Cell Res, 129 (1), 239-49
DOI 10.1016/0014-4827(80)90347-x, PubMed 6159218

Grinde B, Seglen PO (1980)
Differential effects of proteinase inhibitors and amines on the lysosomal and non-lysosomal pathways of protein degradation in isolated rat hepatocytes
Biochim Biophys Acta, 632 (1), 73-86
DOI 10.1016/0304-4165(80)90250-0, PubMed 7417519

Schwarze PE, Seglen PO (1980)
Protein metabolism and survival of rat hepatocytes in early culture
Exp Cell Res, 130 (1), 185-90
DOI 10.1016/0014-4827(80)90055-5, PubMed 6161016

Seglen PO, Gordon PB (1980)
Effects of lysosomotropic monoamines, diamines, amino alcohols, and other amino compounds on protein degradation and protein synthesis in isolated rat hepatocytes
Mol Pharmacol, 18 (3), 468-75
PubMed 7464813

Seglen PO, Gordon PB, Poli A (1980)
Amino acid inhibition of the autophagic/lysosomal pathway of protein degradation in isolated rat hepatocytes
Biochim Biophys Acta, 630 (1), 103-18
DOI 10.1016/0304-4165(80)90141-5, PubMed 7388042

Seglen PO, Solheim AE, Grinde B, Gordon PB, Schwarze PE, Gjessing R, Poli A (1980)
Amino acid control of protein synthesis and degradation in isolated rat hepatocytes
Ann N Y Acad Sci, 349, 1-17
DOI 10.1111/j.1749-6632.1980.tb29510.x, PubMed 6939359

Solheim AE, Seglen PO (1980)
Subcellular distribution of proteolytically generated valine in isolated rat hepatocytes
Eur J Biochem, 107 (2), 587-96
DOI 10.1111/j.1432-1033.1980.tb06067.x, PubMed 7398655

Publications 1979

Rønning OW, Pettersen EO, Seglen PO (1979)
Protein synthesis and protein degradation through the cell cycle of human NHIK 3025 cells in vitro
Exp Cell Res, 123 (1), 63-72
DOI 10.1016/0014-4827(79)90421-x, PubMed 488184

Seglen PO (1979)
Hepatocyte suspensions and cultures as tools in experimental carcinogenesis
J Toxicol Environ Health, 5 (2-3), 551-60
DOI 10.1080/15287397909529766, PubMed 224209

Seglen PO, Gordon PB (1979)
Inhibition of cell spreading by lysosomotropic amines
FEBS Lett, 105 (2), 345-8
DOI 10.1016/0014-5793(79)80645-6, PubMed 488362

Seglen PO, Grinde B, Solheim AE (1979)
Inhibition of the lysosomal pathway of protein degradation in isolated rat hepatocytes by ammonia, methylamine, chloroquine and leupeptin
Eur J Biochem, 95 (2), 215-25
DOI 10.1111/j.1432-1033.1979.tb12956.x, PubMed 456353

Publications 1978

Seglen PO (1978)
Effects of amino acids, ammonia and leupeptin on protein synthesis and degradation in isolated rat hepatocytes
Biochem J, 174 (2), 469-74
DOI 10.1042/bj1740469, PubMed 708399

Seglen PO, Fossa J (1978)
Attachment of rat hepatocytes in vitro to substrata of serum protein, collagen, or concanavalin A
Exp Cell Res, 116 (1), 199-206
DOI 10.1016/0014-4827(78)90076-9, PubMed 29767

Seglen PO, Gjessing R (1978)
Effect of temperature and divalent cations on the substratum attachment of rat hepatocytes in vitro
J Cell Sci, 34, 117-31
DOI 10.1242/jcs.34.1.117, PubMed 748333

Seglen PO, Solheim AE (1978)
Valine uptake and incorporation into protein in isolated rat hepatocytes. Nature of the precursor pool for protein synthesis
Eur J Biochem, 85 (1), 15-25
DOI 10.1111/j.1432-1033.1978.tb12208.x, PubMed 639812

Seglen PO, Solheim AE (1978)
Effects of aminooxyacetate, alanine and other amino acids on protein synthesis in isolated rat hepatocytes
Biochim Biophys Acta, 520 (3), 630-41
DOI 10.1016/0005-2787(78)90148-x, PubMed 718918

Publications 1977

Seglen PO (1977)
Inhibitor of protein degradation formed during incubation of isolated rat hepatocytes in a cell culture medium. Its identification as ammonia
Exp Cell Res, 107 (1), 207-17
DOI 10.1016/0014-4827(77)90402-5, PubMed 16760

Seglen PO (1977)
Protein-catabolic stage of isolated rat hepatocytes
Biochim Biophys Acta, 496 (1), 182-91
DOI 10.1016/0304-4165(77)90126-x, PubMed 836893

Seglen PO, Reith A (1977)
Ammonia inhibits protein secretion in isolated rat hepatocytes
Biochim Biophys Acta, 496 (1), 29-35
DOI 10.1016/0304-4165(77)90112-x, PubMed 836896

Seglen PO, Solheim AE (1977)
Protein degradation in isolated rat hepatocytes
Acta Biol Med Ger, 36 (11-12), 1789-804
PubMed 616724

Publications 1976

Reith A, Seglen PO (1976)
[Ammonia inhibition of protein degradation in isolated rat hepatocytes: quantitative alterations in the lysosomal system (author's transl)]
Verh Dtsch Ges Pathol, 223-6
PubMed 1035828

Seglen PO (1976)
Preparation of isolated rat liver cells
Methods Cell Biol, 13, 29-83
DOI 10.1016/s0091-679x(08)61797-5, PubMed 177845

Seglen PO (1976)
Incorporation of radioactive amino acids into protein in isolated rat hepatocytes
Biochim Biophys Acta, 442 (3), 391-404
DOI 10.1016/0005-2787(76)90313-0, PubMed 963056

Seglen PO, Reith A (1976)
Ammonia inhibition of protein degradation in isolated rat hepatocytes. Quantitative ultrastructural alterations in the lysosomal system
Exp Cell Res, 100 (2), 276-80
DOI 10.1016/0014-4827(76)90148-8, PubMed 939253

Publications 1975

Munthe-Kaas AC, Berg T, Seglen PO, Seljelid R (1975)
Mass isolation and culture of rat kupffer cells
J Exp Med, 141 (1), 1-10
DOI 10.1084/jem.141.1.1, PubMed 1090696

Seglen PO (1975)
[The university's odontological collection]
Nor Tannlaegeforen Tid, 85 (6), 236-40
PubMed 1094410

Seglen PO (1975)
Protein degradation in isolated rat hepatocytes is inhibited by ammonia
Biochem Biophys Res Commun, 66 (1), 44-52
DOI 10.1016/s0006-291x(75)80292-0, PubMed 1164434

Publications 1974

Munthe-Kaas AC, Seglen PO (1974)
The use of Metrizamide as a gradient medium for isopycnic separation of rat liver cells
FEBS Lett, 43 (3), 252-6
DOI 10.1016/0014-5793(74)80654-x, PubMed 4416578

Seglen PO (1974)
Proceedings: Carbohydrate metabolism of isolated parenchymal rat liver cells
Hoppe Seylers Z Physiol Chem, 355 (10), 1255
PubMed 4461596

Seglen PO (1974)
[The pelican]
Tid Tann, 35 (2), 92-7
PubMed 4527569

Seglen PO (1974)
Control of glycogen metabolism in isolated rat liver cells by glucose, insulin and glucagon
Acta Endocrinol Suppl (Copenh), 191, 153-8
DOI 10.1530/acta.0.077s0153, PubMed 4548796

Seglen PO (1974)
Differones: Control of gene expression and cellular differentiation by hormones and other agents, with particular emphasis on liver tissue
Universitetsforl., Oslo, [1] bl., 131 s.
BIBSYS 011869836

Seglen PO (1974)
Hormonal control of gene expression
Norsk Hydro's institutt for kreftforskning, Oslo, Bl.359 s.+13 særtr.
BIBSYS 930873009

Publications 1973

Seglen PO (1973)
Preparation of rat liver cells. 3. Enzymatic requirements for tissue dispersion
Exp Cell Res, 82 (2), 391-8
DOI 10.1016/0014-4827(73)90357-1, PubMed 4358115

Seglen PO (1973)
Preparation of rat liver cells. II. Effects of ions and chelators on tissue dispersion
Exp Cell Res, 76 (1), 25-30
DOI 10.1016/0014-4827(73)90414-x, PubMed 4630105

Seglen PO (1973)
Effects of anaerobiosis, glucose, insulin and glucagon on glycogen metabolism in isolated parenchymal rat liver cells
FEBS Lett, 36 (3), 309-12
DOI 10.1016/0014-5793(73)80398-9, PubMed 4763310

Seglen PO (1973)
Glycogen synthesis in isolated parenchymal rat liver cells
FEBS Lett, 30 (1), 25-28
DOI 10.1016/0014-5793(73)80611-8, PubMed 11947054

Publications 1972

Berg T, Boman D, Seglen PO (1972)
Induction of tryptophan oxygenase in primary rat liver cell suspensions by glucocorticoid hormone
Exp Cell Res, 72 (2), 571-4
DOI 10.1016/0014-4827(72)90034-1, PubMed 5037935

Christoffersen T, Morland J, Osnes JB, Berg T, Boman D, Seglen PO (1972)
Enrichment of glucagon sensitive adenyl cyclase upon preparation of parenchymal cells from rat liver
Arch Biochem Biophys, 150 (2), 807-9
DOI 10.1016/0003-9861(72)90102-6, PubMed 5044053

Morland J, Christoffersen T, Osnes JB, Seglen PO, Jervell KF (1972)
An effect of ethanol administration on tryptophan oxygenase in the perfused rat liver. Decreased enzyme synthesis dependent on low perfusate pH
Biochem Pharmacol, 21 (13), 1849-59
DOI 10.1016/0006-2952(72)90181-5, PubMed 4405195

Seglen PO (1972)
Preparation of rat liver cells. I. Effect of Ca 2+ on enzymatic dispersion of isolated, perfused liver
Exp Cell Res, 74 (2), 450-4
DOI 10.1016/0014-4827(72)90400-4, PubMed 4343020

Seglen PO (1972)
The effect of perfusate pH on respiration and glycolysis in the isolated rat liver perfused with an erythrocyte- and protein-free medium
Biochim Biophys Acta, 264 (3), 398-410
DOI 10.1016/0304-4165(72)90002-5, PubMed 5027624

Publications 1971

Ekren T, Jervell KF, Seglen PO (1971)
Insulin and amino-acid regulation of polysomes in perfused, diabetic rat liver
Nat New Biol, 229 (8), 244-5
DOI 10.1038/newbio229244a0, PubMed 5279798

Seglen PO (1971)
Regulation of tyrosine transaminase degradation in the isolated perfused rat liver by cycloheximide and insulin
Biochim Biophys Acta, 230 (2), 319-26
DOI 10.1016/0304-4165(71)90218-2, PubMed 4396768

Seglen PO (1971)
[Extraction devices]
Tid Tann, 32 (5), 273-9
PubMed 5292780

Seglen PO (1971)
[Dental extraction forceps]
Tid Tann, 32, 273-9
PubMed 11633374

Publications 1970

Berg T, Morland J, Seglen PO (1970)
An effect of glucocorticoid hormone on the nuclear ribonucleoprotein of rat liver
Hoppe Seylers Z Physiol Chem, 351 (7), 898-900
PubMed 5432756

Seglen P (1970)
[Drug effects on enzyme synthesis]
Tidsskr Nor Laegeforen, 90 (2), 208-10
PubMed 4914233

Publications 1969

Jevell KF, Seglen PO (1969)
Tyrosine transaminase degradation in perfused liver after inhibition of protein synthesis by cycloheximide
Biochim Biophys Acta, 174 (1), 398-400
DOI 10.1016/0005-2787(69)90266-4, PubMed 4387935

Seglen PO, Jervell KF (1969)
Tryptophan oxygenase activation and ascorbate oxidation in whole homogenates from rat liver
Biochim Biophys Acta, 171 (1), 47-57
DOI 10.1016/0005-2744(69)90104-1, PubMed 5763409

Seglen PO, Jervell KF (1969)
A simple perfusion technique applied to glucocorticoid regulation of tryptophan oxygenase turnover and bile production in the isolated rat liver
Hoppe Seylers Z Physiol Chem, 350 (3), 308-16
DOI 10.1515/bchm2.1969.350.1.308, PubMed 5769958

Publications 1968

Seglen PO (1968)
Insulin inhibition of tyrosine transaminase degradation in the isolated, perfused rat liver
Hoppe Seylers Z Physiol Chem, 349 (9), 1229-30
PubMed 4387600

Seglen PO (1968)
[The history of instruments for cleaning the teeth]
Nor Tannlaegeforen Tid, 78 (5), 301-8
PubMed 4876473

Seglen PO (1968)
Tannrensingsinstrumentenes historie
Fabritius, Oslo, S. 301-308
BIBSYS 021438315

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