Lymphocytes characterization by CyTOF
In collaboration with Kjersti Flatmark’s group at the department of Tumor Biology at the OUS Radium Hospital we have used cytometry by time of flight (CyTOF) to investigate if lymphocytes infiltrate abdominal cancer metastases and to attempt to phenotype these tumor-infiltrating lymphocytes (TILs) using a panel of 37 extracellular markers. We were able to detect TILs in over half of our samples in the pilot experiment, and T-, B- and NK-cell subpopulations could be identified. Currently we are collecting more samples and performing further testing.
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T-cell, B-cell and NK-cell populations in a patient sample. Subpopulations are clustered using the viSNE analysis tool from Cytobank. CD45 staining is shown.
Additionally, we are also collaborating with Anne Hansens Ree’s group at the department of Oncology at Akershus University Hospital, to use CyTOF to characterize PBMCs in colon cancer patients.