Kirsten Jane Skarstad
- Group leader; Dr. philos.
- +47 976 82 296
Mini CV
- Graduate engineer from the Norwegian Institute of Technology 1981;
- Dr. philos from the University of Oslo, 1987,
- Postdoctoral fellow in professor Arthur Kornberg´s laboratory, Stanford University Medical School, 1988-1991;
- Group leader, Department of Biophysics, Institute for Cancer Research, Norwegian Radium Hospital, 1991-1996;
- Group leader, the Department of Cell Biology, Institute for Cancer Research, Norwegian Radium Hospital, 1996-present;
- Adjunct professor at the Institute of Pharmacy, University of Oslo, 2000-present.
- Member of the Board of the Science and Technology Division, The Norwegian Research Council, 2001-2003.
- Member of the Evaluation Committee for the Norwegian Science and Technology Centers of Excellence, 2001-2002.
- Member of the EU project QLK2-CT-2000-00634, 2000-2004
- Member of FUGE platform CAMST (Advanced Microbial Science and Technology) 2003-2008
- Member of European Molecular Biology Organization (EMBO), 2004
- Member of the Norwegian Academy of Science and Letters (Det Norske Vitenskaps-Akademi), 2008
- Member of the EMBO Fellowship Committee, 2009-2012
Publications 2023
A DnaA-dependent riboswitch for transcription attenuation of the his operon
mLife, 2 (2), 126-140
DOI 10.1002/mlf2.12075, PubMed 38817620
A DnaA‐dependent riboswitch for transcription attenuation of the his operon
mLife, 2 (2), 126-140
DOI 10.1002/mlf2.12075, PublikaID 479
Publications 2021
Topoisomerase IV tracks behind the replication fork and the SeqA complex during DNA replication in Escherichia coli
Sci Rep, 11 (1), 474
DOI 10.1038/s41598-020-80043-4, PubMed 33436807
Antimicrobial resistance: A challenge awaiting the post-COVID-19 era
Int J Infect Dis, 111, 322-325
DOI 10.1016/j.ijid.2021.09.003, PubMed 34508864
Publications 2020
Antibiotic-induced DNA damage results in a controlled loss of pH homeostasis and genome instability
Sci Rep, 10 (1), 19422
DOI 10.1038/s41598-020-76426-2, PubMed 33173044
Peptides containing the PCNA interacting motif APIM bind to the β-clamp and inhibit bacterial growth and mutagenesis
Nucleic Acids Res, 48 (10), 5540-5554
DOI 10.1093/nar/gkaa278, PubMed 32347931
Publications 2018
Multiple links connect central carbon metabolism to DNA replication initiation and elongation in Bacillus subtilis
DNA Res, 25 (6), 641-653
DOI 10.1093/dnares/dsy031, PubMed 30256918
Publications 2017
Phenotypes of dnaXE145A Mutant Cells Indicate that the Escherichia coli Clamp Loader Has a Role in the Restart of Stalled Replication Forks
J Bacteriol, 199 (24)
DOI 10.1128/JB.00412-17, PubMed 28947673
SeqA structures behind Escherichia coli replication forks affect replication elongation and restart mechanisms
Nucleic Acids Res, 45 (11), 6471-6485
DOI 10.1093/nar/gkx263, PubMed 28407100
Publications 2016
Lack of the H-NS Protein Results in Extended and Aberrantly Positioned DNA during Chromosome Replication and Segregation in Escherichia coli
J Bacteriol, 198 (8), 1305-16
DOI 10.1128/JB.00919-15, PubMed 26858102
Publications 2015
The DnaA Protein Is Not the Limiting Factor for Initiation of Replication in Escherichia coli
PLoS Genet, 11 (6), e1005276
DOI 10.1371/journal.pgen.1005276, PubMed 26047361
Dynamic Escherichia coli SeqA complexes organize the newly replicated DNA at a considerable distance from the replisome
Nucleic Acids Res, 43 (5), 2730-43
DOI 10.1093/nar/gkv146, PubMed 25722374
Publications 2014
Escherichia coli SeqA structures relocalize abruptly upon termination of origin sequestration during multifork DNA replication
PLoS One, 9 (10), e110575
DOI 10.1371/journal.pone.0110575, PubMed 25333813
The Escherichia coli datA site promotes proper regulation of cell division
Microbiology (Reading), 160 (Pt 4), 703-710
DOI 10.1099/mic.0.074898-0, PubMed 24574433
DNA compaction in the early part of the SOS response is dependent on RecN and RecA
Microbiology (Reading), 160 (Pt 5), 872-882
DOI 10.1099/mic.0.075051-0, PubMed 24615185
Publications 2013
The Fis protein has a stimulating role in initiation of replication in Escherichia coli in vivo
PLoS One, 8 (12), e83562
DOI 10.1371/journal.pone.0083562, PubMed 24358293
Regulating DNA replication in bacteria
Cold Spring Harb Perspect Biol, 5 (4), a012922
DOI 10.1101/cshperspect.a012922, PubMed 23471435
Single transmembrane peptide DinQ modulates membrane-dependent activities
PLoS Genet, 9 (2), e1003260
DOI 10.1371/journal.pgen.1003260, PubMed 23408903
Publications 2012
Depletion of acidic phospholipids influences chromosomal replication in Escherichia coli
Microbiologyopen, 1 (4), 450-66
DOI 10.1002/mbo3.46, PubMed 23233230
An easy-to-use simulation program demonstrates variations in bacterial cell cycle parameters depending on medium and temperature
PLoS One, 7 (2), e30981
DOI 10.1371/journal.pone.0030981, PubMed 22348034
Replication fork movement and methylation govern SeqA binding to the Escherichia coli chromosome
Nucleic Acids Res, 40 (12), 5465-76
DOI 10.1093/nar/gks187, PubMed 22373925
Publications 2011
Hydrophilic interaction chromatography of nucleoside triphosphates with temperature as a separation parameter
J Chromatogr A, 1218 (35), 5981-6
DOI 10.1016/j.chroma.2011.01.066, PubMed 21315360
Publications 2010
The G157C mutation in the Escherichia coli sliding clamp specifically affects initiation of replication
Mol Microbiol, 79 (2), 433-46
DOI 10.1111/j.1365-2958.2010.07453.x, PubMed 21219462
A novel DNA gyrase inhibitor rescues Escherichia coli dnaAcos mutant cells from lethal hyperinitiation
J Antimicrob Chemother, 65 (5), 924-30
DOI 10.1093/jac/dkq071, PubMed 20228083
Replication patterns and organization of replication forks in Vibrio cholerae
Microbiology (Reading), 157 (Pt 3), 695-708
DOI 10.1099/mic.0.045112-0, PubMed 21163839
ChIP on Chip: surprising results are often artifacts
BMC Genomics, 11, 414
DOI 10.1186/1471-2164-11-414, PubMed 20602746
Publications 2009
Escherichia coli DnaA forms helical structures along the longitudinal cell axis distinct from MreB filaments
Mol Microbiol, 72 (3), 645-57
DOI 10.1111/j.1365-2958.2009.06674.x, PubMed 19400775
Growth rate dependent numbers of SeqA structures organize the multiple replication forks in rapidly growing Escherichia coli
Genes Cells, 14 (5), 643-57
DOI 10.1111/j.1365-2443.2009.01298.x, PubMed 19371375
A reduction in ribonucleotide reductase activity slows down the chromosome replication fork but does not change its localization
PLoS One, 4 (10), e7617
DOI 10.1371/journal.pone.0007617, PubMed 19898675
The Escherichia coli SeqA protein
Plasmid, 61 (3), 141-50
DOI 10.1016/j.plasmid.2009.02.004, PubMed 19254745
Publications 2008
The initiator protein DnaA contributes to keeping new origins inactivated by promoting the presence of hemimethylated DNA
J Mol Biol, 384 (5), 1076-85
DOI 10.1016/j.jmb.2008.09.042, PubMed 18835566
DnaA protein interacts with RNA polymerase and partially protects it from the effect of rifampicin
Mol Microbiol, 71 (4), 1018-30
DOI 10.1111/j.1365-2958.2008.06585.x, PubMed 19170875
A robust screen for novel antibiotics: specific knockout of the initiator of bacterial DNA replication
FEMS Microbiol Lett, 281 (2), 210-4
DOI 10.1111/j.1574-6968.2008.01103.x, PubMed 18312360
Publications 2007
Organization of sister origins and replisomes during multifork DNA replication in Escherichia coli
EMBO J, 26 (21), 4514-22
DOI 10.1038/sj.emboj.7601871, PubMed 17914458
Functional taxonomy of bacterial hyperstructures
Microbiol Mol Biol Rev, 71 (1), 230-53
DOI 10.1128/MMBR.00035-06, PubMed 17347523
Toward a hyperstructure taxonomy
Annu Rev Microbiol, 61, 309-29
DOI 10.1146/annurev.micro.61.081606.103348, PubMed 17896876
Publications 2006
A novel regulatory mechanism couples deoxyribonucleotide synthesis and DNA replication in Escherichia coli
EMBO J, 25 (5), 1137-47
DOI 10.1038/sj.emboj.7600990, PubMed 16482221
Publications 2005
An oriC-like distribution of GATC sites mediates full sequestration of non-origin sequences in Escherichia coli
J Mol Biol, 350 (1), 7-11
DOI 10.1016/j.jmb.2005.04.055, PubMed 15922360
Deletion of the datA site does not affect once-per-cell-cycle timing but induces rifampin-resistant replication
J Bacteriol, 187 (12), 3913-20
DOI 10.1128/JB.187.12.3913-3920.2005, PubMed 15939703
Specific N-terminal interactions of the Escherichia coli SeqA protein are required to form multimers that restrain negative supercoils and form foci
Genes Cells, 10 (11), 1039-49
DOI 10.1111/j.1365-2443.2005.00898.x, PubMed 16236133
Publications 2004
Re-replication from non-sequesterable origins generates three-nucleoid cells which divide asymmetrically
Mol Microbiol, 51 (6), 1589-600
DOI 10.1111/j.1365-2958.2003.03943.x, PubMed 15009887
Positive supercoiling is generated in the presence of Escherichia coli SeqA protein
Mol Microbiol, 54 (1), 123-31
DOI 10.1111/j.1365-2958.2004.04239.x, PubMed 15458410
Replication fork and SeqA focus distributions in Escherichia coli suggest a replication hyperstructure dependent on nucleotide metabolism
Mol Microbiol, 52 (6), 1597-612
DOI 10.1111/j.1365-2958.2004.04097.x, PubMed 15186411
Publications 2003
Excess SeqA prolongs sequestration of oriC and delays nucleoid segregation and cell division
EMBO J, 22 (2), 315-23
DOI 10.1093/emboj/cdg020, PubMed 12514137
Controlled initiation of chromosomal replication in Escherichia coli requires functional Hda protein
J Bacteriol, 185 (10), 3244-8
DOI 10.1128/JB.185.10.3244-3248.2003, PubMed 12730188
Lack of SeqA focus formation, specific DNA binding and proper protein multimerization in the Escherichia coli sequestration mutant seqA2
Mol Microbiol, 47 (3), 619-32
DOI 10.1046/j.1365-2958.2003.t01-1-03329.x, PubMed 12535065
SeqA protein stimulates the relaxing and decatenating activities of topoisomerase IV
J Biol Chem, 278 (49), 48779-85
DOI 10.1074/jbc.M308843200, PubMed 14512422
DpiA binding to the replication origin of Escherichia coli plasmids and chromosomes destabilizes plasmid inheritance and induces the bacterial SOS response
J Bacteriol, 185 (20), 6025-31
DOI 10.1128/JB.185.20.6025-6031.2003, PubMed 14526013
Titration of the Escherichia coli DnaA protein to excess datA sites causes destabilization of replication forks, delayed replication initiation and delayed cell division
Mol Microbiol, 50 (1), 349-62
DOI 10.1046/j.1365-2958.2003.03695.x, PubMed 14507385
Circles in the sand
EMBO Rep, 4 (7), 661-5
DOI 10.1038/sj.embor.embor888, PubMed 12835752
Stable co-existence of separate replicons in Escherichia coli is dependent on once-per-cell-cycle initiation
EMBO J, 22 (1), 140-50
DOI 10.1093/emboj/cdg003, PubMed 12505992
Degradation of mutant initiator protein DnaA204 by proteases ClpP, ClpQ and Lon is prevented when DNA is SeqA-free
Biochem J, 370 (Pt 3), 867-71
DOI 10.1042/BJ20021161, PubMed 12479794
Publications 2001
Regulation of chromosomal replication by DnaA protein availability in Escherichia coli: effects of the datA region
Biochim Biophys Acta, 1521 (1-3), 73-80
DOI 10.1016/s0167-4781(01)00292-5, PubMed 11690638
The Escherichia coli SeqA protein binds specifically to two sites in fully and hemimethylated oriC and has the capacity to inhibit DNA replication and affect chromosome topology
Biochimie, 83 (1), 49-51
DOI 10.1016/s0300-9084(00)01207-4, PubMed 11254974
SeqA, the Escherichia coli origin sequestration protein, is also a specific transcription factor
Mol Microbiol, 40 (6), 1371-9
DOI 10.1046/j.1365-2958.2001.02480.x, PubMed 11442835
Mutations in DnaA protein suppress the growth arrest of acidic phospholipid-deficient Escherichia coli cells
EMBO J, 20 (5), 1164-72
DOI 10.1093/emboj/20.5.1164, PubMed 11230139
Publications 2000
Mapping of a chromosome replication origin in an archaeon
Trends Microbiol, 8 (12), 535-7
DOI 10.1016/s0966-842x(00)01878-3, PubMed 11115744
Limiting DNA replication to once and only once
EMBO Rep, 1 (6), 479-83
DOI 10.1093/embo-reports/kvd116, PubMed 11263490
The Escherichia coli SeqA protein binds specifically and co-operatively to two sites in hemimethylated and fully methylated oriC
Mol Microbiol, 36 (6), 1319-26
DOI 10.1046/j.1365-2958.2000.01943.x, PubMed 10931282
The Escherichia coli SeqA protein destabilizes mutant DnaA204 protein
Mol Microbiol, 37 (3), 629-38
DOI 10.1046/j.1365-2958.2000.02031.x, PubMed 10931356
Publications 1999
Escherichia coli SeqA protein affects DNA topology and inhibits open complex formation at oriC
EMBO J, 18 (17), 4882-8
DOI 10.1093/emboj/18.17.4882, PubMed 10469666
Publications 1998
Effects of purified SeqA protein on oriC-dependent DNA replication in vitro
EMBO J, 17 (14), 4158-65
DOI 10.1093/emboj/17.14.4158, PubMed 9670030
Publications 1997
Uncoupling of the order of the S and M phases: effects of staurosporine on human cell cycle kinases
Cell Prolif, 30 (5), 197-218
DOI 10.1046/j.1365-2184.1997.00087.x, PubMed 9420612
Publications 1996
Coordinating DNA replication initiation with cell growth: differential roles for DnaA and SeqA proteins
Proc Natl Acad Sci U S A, 93 (22), 12206-11
DOI 10.1073/pnas.93.22.12206, PubMed 8901558
Transcriptional activation of promoters of the superoxide and multiple antibiotic resistance regulons by Rob, a binding protein of the Escherichia coli origin of chromosomal replication
J Bacteriol, 178 (9), 2507-13
DOI 10.1128/jb.178.9.2507-2513.1996, PubMed 8626315
The Escherichia coli Fis protein prevents initiation of DNA replication from oriC in vitro
Nucleic Acids Res, 24 (18), 3527-32
DOI 10.1093/nar/24.18.3527, PubMed 8836178
Publications 1995
Analysis of DNA replication in vivo by flow cytometry
Methods Enzymol, 262, 604-13
DOI 10.1016/0076-6879(95)62047-8, PubMed 8594381
The speed of the Escherichia coli fork in vivo depends on the DnaB:DnaC ratio
Mol Microbiol, 17 (5), 825-31
DOI 10.1111/j.1365-2958.1995.mmi_17050825.x, PubMed 8596432
E. coli SeqA protein binds oriC in two different methyl-modulated reactions appropriate to its roles in DNA replication initiation and origin sequestration
Cell, 82 (6), 927-36
DOI 10.1016/0092-8674(95)90272-4, PubMed 7553853
Publications 1994
The initiator protein DnaA: evolution, properties and function
Biochim Biophys Acta, 1217 (2), 111-30
DOI 10.1016/0167-4781(94)90025-6, PubMed 8110826
Staining and measurement of DNA in bacteria
Methods Cell Biol, 42 Pt B, 477-87
DOI 10.1016/s0091-679x(08)61091-2, PubMed 7533253
The initiation mass for DNA replication in Escherichia coli K-12 is dependent on growth rate
EMBO J, 13 (9), 2097-102
DOI 10.1002/j.1460-2075.1994.tb06485.x, PubMed 8187762
Publications 1993
Degradation of individual chromosomes in recA mutants of Escherichia coli
J Bacteriol, 175 (17), 5505-9
DOI 10.1128/jb.175.17.5505-5509.1993, PubMed 8366035
A novel binding protein of the origin of the Escherichia coli chromosome
J Biol Chem, 268 (8), 5365-70
PubMed 8449900
Publications 1991
E. coli minichromosome replication: regulation of initiation at oriC
Res Microbiol, 142 (2-3), 127-30
DOI 10.1016/0923-2508(91)90019-7, PubMed 1925009
Publications 1990
Strand separation required for initiation of replication at the chromosomal origin of E.coli is facilitated by a distant RNA--DNA hybrid
EMBO J, 9 (7), 2341-8
DOI 10.1002/j.1460-2075.1990.tb07406.x, PubMed 1694129
DNA measurements of bacteria
Methods Cell Biol, 33, 519-26
DOI 10.1016/s0091-679x(08)60551-8, PubMed 2084483
Publications 1989
The DnaA protein determines the initiation mass of Escherichia coli K-12
Cell, 57 (5), 881-9
DOI 10.1016/0092-8674(89)90802-7, PubMed 2541928
Initiation of DNA replication in Escherichia coli after overproduction of the DnaA protein
Mol Gen Genet, 218 (1), 50-6
DOI 10.1007/BF00330564, PubMed 2550764
Publications 1988
Timing of chromosomal replication in Escherichia coli
Biochim Biophys Acta, 951 (2-3), 359-64
DOI 10.1016/0167-4781(88)90107-8, PubMed 2850013
Perturbed chromosomal replication in recA mutants of Escherichia coli
J Bacteriol, 170 (6), 2549-54
DOI 10.1128/jb.170.6.2549-2554.1988, PubMed 3286612
Coordination of chromosome replication initiation in Escherichia coli: effects of different dnaA alleles
J Bacteriol, 170 (2), 852-8
DOI 10.1128/jb.170.2.852-858.1988, PubMed 2828328
Publications 1987
Chromosomal DNA replication in Escherichia coli studied by flow cytometry
Department of Biophysics, Institute for Cancer Research, The Norwegian Radium Hospital and The Norwegian Cancer Society, Oslo, 1 b. (flere pag.)
BIBSYS 872208214, ISBN 82-90187-38-6
Mode of initiation of constitutive stable DNA replication in RNase H-defective mutants of Escherichia coli K-12
J Bacteriol, 169 (6), 2650-8
DOI 10.1128/jb.169.6.2650-2658.1987, PubMed 3034862
Publications 1986
Timing of initiation of chromosome replication in individual Escherichia coli cells
EMBO J, 5 (7), 1711-7
DOI 10.1002/j.1460-2075.1986.tb04415.x, PubMed 3527695
Flow cytometry of bacteria: cell cycle kinetics and effects of antibiotics
Ann N Y Acad Sci, 468, 329-38
DOI 10.1111/j.1749-6632.1986.tb42050.x, PubMed 2942091
Publications 1985
RecA protein acts at the initiation of stable DNA replication in rnh mutants of Escherichia coli K-12
J Bacteriol, 163 (2), 439-44
DOI 10.1128/jb.163.2.439-444.1985, PubMed 2991187
Escherichia coli DNA distributions measured by flow cytometry and compared with theoretical computer simulations
J Bacteriol, 163 (2), 661-8
DOI 10.1128/jb.163.2.661-668.1985, PubMed 3894332
Publications 1984
Characterization of intracellular DNA strand breaks induced by neocarzinostatin in Escherichia coli cells
Nucleic Acids Res, 12 (21), 8281-91
DOI 10.1093/nar/12.21.8281, PubMed 6239141
Publications 1983
Flow cytometry of bacteria: a promising tool in experimental and clinical microbiology
J Gen Microbiol, 129 (4), 973-80
DOI 10.1099/00221287-129-4-973, PubMed 6350534
Cell cycle parameters of slowly growing Escherichia coli B/r studied by flow cytometry
J Bacteriol, 154 (2), 656-62
DOI 10.1128/jb.154.2.656-662.1983, PubMed 6341358
Publications 1982
Applications of flow cytometry on bacteria: cell cycle kinetics, drug effects, and quantitation of antibody binding
Cytometry, 2 (4), 249-57
DOI 10.1002/cyto.990020409, PubMed 7035105
Publications 1981
Deoxyribonucleic acid replication in permeable and fully viable Escherichia coli cells
J Bacteriol, 145 (3), 1413-6
DOI 10.1128/jb.145.3.1413-1416.1981, PubMed 7009586
DNA replication i Escherichia coli studert med to forskjellige metoder
Almenavdelingen, NTH, Trondheim, 77 bl
BIBSYS 061950661