
Nikolai Engedal
- Researcher; PhD
- +47 22 78 17 62
Bio
Dr. Engedal joined the Urbanucci lab in the group of Eivind Hovig at the Department of Tumor Biology in July 2019. He is currently exploring epigenetic-targeting drugs for novel chemo/radiation combination therapies in prostate cancer. Other research interests include delineating the role of autophagy in cancer, and the potential use of autophagy modulation in cancer therapy.
Dr Engedal graduated with an MSc in Biochemistry at the University of Oslo, where he studied signaling, transcriptional regulation, and cell death in prostate cancer cells in the group of Professor Fahri Saatcioglu. In 2005, he obtained a PhD (Dr. philos) in Cell Biology/Immunology at the Medical Faculty (University of Oslo), studying the regulation of T cell proliferation and cell death by retinoic acid in the group of Professor Heidi Kiil Blomhoff. Following a postdoc period in the group of Professor Kirsten Sandvig at the Norwegian Radium Hospital, where he studied membrane transport mechanisms and cancer-relevant toxins, he in 2010 joined the group of Professor Ian G. Mills at the Centre for Molecular Medicine Norway (NCMM; a Nordic EMBL partnership). Here Dr Engedal started his own project centered on understanding the molecular regulation of autophagy (a lysosomal degradation pathway for intracellular material) and cell death by calcium and ER stress signaling. For this he received several research grants, including a young talent grant from the Research Council of Norway. The focus on autophagy included a long-term collaboration (since 2012) with the Norwegian pioneer in autophagy research, Professor Per O. Seglen. Dr Engedal led the independent research group “The Autophagy Team” at NCMM in the period 2014-2019, publishing >20 papers in peer-reviewed journals.
Dr Engedal is chairing the Nordic Autophagy Society (since 2015), and is a Norwegian representative in the managing committee of the TransAUTOPHAGY EU COST action CA15138 (since 2016). Dr Engedal is an Editorial Board Member in the journals Cells (ISSN 2073-4409) and Biomolecules (ISSN 2218-273X). Moreover, Engedal has guest edited the Special Issues “Transautophagy: Research and Translation of Autophagy Knowledge” in Oxidative Medicine and Cellular Longevity (ISSN 1942-0900) in 2018 and “Autophagy in Cancer” in Biology (ISSN 2079-7737) in 2019-2020.
Dr Engedal has authored >40 papers in peer-reviewed journals (see list of publications below).
Publications 2020
Assessing Autophagy in Archived Tissue or How to Capture Autophagic Flux from a Tissue Snapshot
Biology (Basel), 9 (3)
DOI 10.3390/biology9030059, PubMed 32245178
Cell death induced by the ER stressor thapsigargin involves death receptor 5, a non-autophagic function of MAP1LC3B, and distinct contributions from unfolded protein response components
Cell Commun Signal, 18 (1), 12
DOI 10.1186/s12964-019-0499-z, PubMed 31987044
Structural Variants of poly(alkylcyanoacrylate) Nanoparticles Differentially Affect LC3 and Autophagic Cargo Degradation
J Biomed Nanotechnol, 16 (4), 432-445
DOI 10.1166/jbn.2020.2906, PubMed 32970976
Publications 2019
Measurement of Bulk Autophagy by a Cargo Sequestration Assay
Methods Mol Biol, 1880, 307-313
DOI 10.1007/978-1-4939-8873-0_20, PubMed 30610706
The kinase PERK and the transcription factor ATF4 play distinct and essential roles in autophagy resulting from tunicamycin-induced ER stress
J Biol Chem, 294 (20), 8197-8217
DOI 10.1074/jbc.RA118.002829, PubMed 30926605
Small variations in nanoparticle structure dictate differential cellular stress responses and mode of cell death
Nanotoxicology, 13 (6), 761-782
DOI 10.1080/17435390.2019.1576238, PubMed 30760074
ESCRT-mediated phagophore sealing during mitophagy
Autophagy, 16 (5), 826-841
DOI 10.1080/15548627.2019.1639301, PubMed 31366282
Publications 2018
Transautophagy: Research and Translation of Autophagy Knowledge
Oxid Med Cell Longev, 2018, 7504165
DOI 10.1155/2018/7504165, PubMed 29861835
From Oxidative Stress Damage to Pathways, Networks, and Autophagy via MicroRNAs
Oxid Med Cell Longev, 2018, 4968321
DOI 10.1155/2018/4968321, PubMed 29849898
The Long-lived Protein Degradation Assay: an Efficient Method for Quantitative Determination of the Autophagic Flux of Endogenous Proteins in Adherent Cell Lines
Bio-protocol, 8 (9), e2836
The Lactate Dehydrogenase Sequestration Assay - A Simple and Reliable Method to Determine Bulk Autophagic Sequestration Activity in Mammalian Cells
J Vis Exp (137)
DOI 10.3791/57971, PubMed 30102280
Chloroquine inhibits autophagic flux by decreasing autophagosome-lysosome fusion
Autophagy, 14 (8), 1435-1455
DOI 10.1080/15548627.2018.1474314, PubMed 29940786
The Role of Free Radicals in Autophagy Regulation: Implications for Ageing
Oxid Med Cell Longev, 2018, 2450748
DOI 10.1155/2018/2450748, PubMed 29682156
The ER Stress Inducer l-Azetidine-2-Carboxylic Acid Elevates the Levels of Phospho-eIF2α and of LC3-II in a Ca2+-Dependent Manner
Cells, 7 (12)
DOI 10.3390/cells7120239, PubMed 30513588
An Image-based Assay for High-throughput Analysis of Cell Proliferation and Cell Death of Adherent Cells
Bio-protocol, 8 (9), e2835
Nonlinear relationship between ER Ca2+ depletion versus induction of the unfolded protein response, autophagy inhibition, and cell death
Cell Calcium, 76, 48-61
DOI 10.1016/j.ceca.2018.09.005, PubMed 30261424
Publications 2017
Rab7b modulates autophagic flux by interacting with Atg4B
EMBO Rep, 18 (10), 1727-1739
DOI 10.15252/embr.201744069, PubMed 28835545
Inhibition of the sarco/endoplasmic reticulum (ER) Ca2+-ATPase by thapsigargin analogs induces cell death via ER Ca2+ depletion and the unfolded protein response
J Biol Chem, 292 (48), 19656-19673
DOI 10.1074/jbc.M117.796920, PubMed 28972171
A Novel Role of Listeria monocytogenes Membrane Vesicles in Inhibition of Autophagy and Cell Death
Front Cell Infect Microbiol, 7, 154
DOI 10.3389/fcimb.2017.00154, PubMed 28516064
Publications 2016
Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)
Autophagy, 12 (1), 1-222
DOI 10.1080/15548627.2015.1100356, PubMed 26799652
A Simple Cargo Sequestration Assay for Quantitative Measurement of Nonselective Autophagy in Cultured Cells
Methods Enzymol, 587, 351-364
DOI 10.1016/bs.mie.2016.09.064, PubMed 28253965
Publications 2015
Autophagy of cytoplasmic bulk cargo does not require LC3
Autophagy, 12 (2), 439-41
DOI 10.1080/15548627.2015.1076606, PubMed 26237084
Novel steps in the autophagic-lysosomal pathway
FEBS J, 282 (11), 2202-14
DOI 10.1111/febs.13268, PubMed 25779646
Macroautophagic cargo sequestration assays
Methods, 75, 25-36
DOI 10.1016/j.ymeth.2014.12.021, PubMed 25576638
Autophagic bulk sequestration of cytosolic cargo is independent of LC3, but requires GABARAPs
Exp Cell Res, 333 (1), 21-38
DOI 10.1016/j.yexcr.2015.02.003, PubMed 25684710
Publications 2014
Endosomal signaling and oncogenesis
Methods Enzymol, 535, 179-200
DOI 10.1016/B978-0-12-397925-4.00012-2, PubMed 24377925
UAP1 is overexpressed in prostate cancer and is protective against inhibitors of N-linked glycosylation
Oncogene, 34 (28), 3744-50
DOI 10.1038/onc.2014.307, PubMed 25241896
Publications 2013
Modulation of intracellular calcium homeostasis blocks autophagosome formation
Autophagy, 9 (10), 1475-90
DOI 10.4161/auto.25900, PubMed 23970164
The ERM proteins ezrin and moesin regulate retrograde Shiga toxin transport
Traffic, 14 (7), 839-52
DOI 10.1111/tra.12077, PubMed 23593995
Targeting autophagy potentiates the apoptotic effect of histone deacetylase inhibitors in t(8;21) AML cells
Blood, 122 (14), 2467-76
DOI 10.1182/blood-2013-05-500629, PubMed 23970379
Publications 2011
Immune regulator vitamin A and T cell death
Vitam Horm, 86, 153-78
DOI 10.1016/B978-0-12-386960-9.00007-1, PubMed 21419271
Shiga toxin and its use in targeted cancer therapy and imaging
Microb Biotechnol, 4 (1), 32-46
DOI 10.1111/j.1751-7915.2010.00180.x, PubMed 21255370
Publications 2010
Protein toxins from plants and bacteria: probes for intracellular transport and tools in medicine
FEBS Lett, 584 (12), 2626-34
DOI 10.1016/j.febslet.2010.04.008, PubMed 20385131
The Intracellular Journey of Shiga Toxins
The Open Toxinology Journal, 3, 3-12
PublikaID 24
Toll-like receptor 4 facilitates binding of Shiga toxin to colon carcinoma and primary umbilical vein endothelial cells
FEMS Immunol Med Microbiol, 61 (1), 63-75
DOI 10.1111/j.1574-695X.2010.00749.x, PubMed 21054580
Publications 2008
Retinoic acid regulates Fas-induced apoptosis in Jurkat T cells: reversal of mitogen-mediated repression of Fas DISC assembly
J Leukoc Biol, 85 (3), 469-80
DOI 10.1189/jlb.1107790, PubMed 19112091
Publications 2006
All-trans retinoic acid stimulates IL-2-mediated proliferation of human T lymphocytes: early induction of cyclin D3
J Immunol, 177 (5), 2851-61
DOI 10.4049/jimmunol.177.5.2851, PubMed 16920920
Publications 2004
Survival of activated human T lymphocytes is promoted by retinoic acid via induction of IL-2
Int Immunol, 16 (3), 443-53
DOI 10.1093/intimm/dxh048, PubMed 14978018
Publications 2003
Combined action of ERK and NF kappa B mediates the protective effect of phorbol ester on Fas-induced apoptosis in Jurkat cells
J Biol Chem, 278 (13), 10934-41
DOI 10.1074/jbc.M211556200, PubMed 12551910
Publications 2002
C-Jun N-terminal kinase is required for phorbol ester- and thapsigargin-induced apoptosis in the androgen responsive prostate cancer cell line LNCaP
Oncogene, 21 (7), 1017-27
DOI 10.1038/sj.onc.1205167, PubMed 11850819
Retinoic acid stimulates the cell cycle machinery in normal T cells: involvement of retinoic acid receptor-mediated IL-2 secretion
J Immunol, 169 (10), 5555-63
DOI 10.4049/jimmunol.169.10.5555, PubMed 12421932
Publications 2001
Ceramide-induced cell death in the prostate cancer cell line LNCaP has both necrotic and apoptotic features
Prostate, 46 (4), 289-97
DOI 10.1002/1097-0045(20010301)46:4<289::aid-pros1035>3.0.co;2-k, PubMed 11241551
Publications 2000
Efficient DNA-mediated gene transfer into prostate cancer cell line LNCaP
Prostate, 43 (2), 111-7
DOI 10.1002/(sici)1097-0045(20000501)43:2<111::aid-pros5>3.0.co;2-4, PubMed 10754526
Publications 1998
CREB binding protein is a coactivator for the androgen receptor and mediates cross-talk with AP-1
J Biol Chem, 273 (48), 31853-9
DOI 10.1074/jbc.273.48.31853, PubMed 9822653