Our research activities are divided into three subprojects and include studies of effects of ionizing radiation on malignant cells and tissues, as well as studies of physiological and biological processes in tumors of relevance for radiation therapy of cancer. The aims of the three subprojects are:
I. To establish diagnostic methods for detecting radiation resistant tumors due to hypoxic regions or high interstitial fluid pressure.
II. To use intravital microscopy for studying tumor angiogenesis and physiology, and to develop therapeutic strategies for overcoming hypoxia-induced radiation resistance.
III. To discover mechanisms of microenvironment- and/or radiation therapy-induced metastasis.
The experiments are being performed with human melanomas and cervix carcinomas (cell lines, multicellular spheroids, xenografts, tumor biopsies and patients with cervix carcinomas). Important methods include intravital microscopy, pO2 histography (Eppendorf and OxyLite electrodes), HbO2 microspectrophotometry, wick-in-needle and micropipette measurements of interstitial fluid pressure, MR imaging and spectroscopy, and several techniques for studying the molecular biology of tumors).
Intravital microscopy of tumor vessel morphology and function using a standard fluorescence microscope
Eur J Nucl Med Mol Imaging (in press)
DCE-MRI of Tumor Hypoxia and Hypoxia-Associated Aggressiveness
Cancers (Basel), 12 (7)
Antifibrotic therapy to normalize the tumor microenvironment
J Transl Med, 18 (1), 207