Tumor models

Upper panel: Athymic nude mouse with a  human melanoma xenograft. Lower panel: Multicellular melanoma  spheroids growing  in vitro. The cells are labeled with green fluorescence protein (GFP).
Upper panel: Athymic nude mouse with a human melanoma xenograft. Lower panel: Multicellular melanoma spheroids growing in vitro. The cells are labeled with green fluorescence protein (GFP).

Human Tumor Xenografts

We use human melanoma and cervix carcinoma xenografts as experimental in vivo models of human cancer. The tumors are implanted orthotopically in athymic nude mice (BALB/c-nu/nu mice). We have demonstrated that our xenografted tumors retain several essential biological features of the donor patientsÂ’ tumors, including many pathophysiological characteristics, response to treatment, and an organ-specific metastatic pattern.


Multicellular Spheroids

We use human melanoma multicellular spheroids as experimental in vitro models of human cancer. The spheroids are grown in liquid-overlay culture or in spinner flasks


GFP-labeled Melanoma Cells

We use human melanoma cells labeled with green fluorescence protein (GFP) to study metastatic dissemination and growth. The GFP is unique among light-emitting proteins in that it does not require any cofactors or substrates for generation of green light. Light generation is triggered by simple exposure of the GFP to standard long-wave ultraviolet light.
 
Mar 22, 2004 Page visits: 7519