Video and image gallery
EGF in HeLa cells.
Alexa488-labelled EGF was added to HeLa cells and internalized. The cells were then imaged by the Zeiss LSM Live scanner. Three-dimensional stacks were taken every minute. The green dots represent EGF in endosomes/lysosomes. The nucleus (in blue) is stained with Hoechst 33342. Made by Jørgen Wesche.
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HRS in microdomains on enlarged endosomes.
Three-dimensional reconstruction from image stacks of cells transfected with hrs-GFP. Hrs-GFP can be seen as microdomains on the surface of enlarged endosomes. The endosomes was enlarged by co-transfecting with constitutively active Rab5. Made by Camilla Raiborg and Jørgen Wesche.
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Internalization of EGF.
Fluorophore-labeled EGF was added to HeLa cells and the endocytosis was followed by time-lapse imaging for 20 minutes. The Zeiss confocal LSM510 was used for imaging. Made by Camilla Raiborg and Jørgen Wesche.
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Wound-healing assay.
After wounding, U2OS cells expressing FGF receptors were stimulated with FGF1 and monitored over time using the Nikon Biostation IM. Made by Angela Oppelt
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Nuclear import assay.
A NLS from FGF1 was fused to GFP and transfected into HeLa cells. The nucleus was bleached and the reappearance of green fluorescence into the nucleus indicates that the fusion protein is imported into the nucleus. Made by Jørgen Wesche.
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Internalization of anthrax toxin protective antigen (PA).
Fluorophore-labeled PA was added to HeLa cells and the intracellular transport was followed by time-lapse imaging. The Zeiss confocal LSM510 was used for imaging. Made by Jørgen Wesche.
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Internalization of anthrax toxin protective antigen (PA).
Fluorophore-labeled PA was added to HeLa cells and the intracellular transport was followed by time-lapse imaging. The Zeiss confocal LSM510 was used for imaging. Made by Jørgen Wesche.
Click here to play movie