Cover Experimental Cell Research
Image made by Dr. Camilla Raiborg.
Cover of Traffic
Cover Legend: Ultrastructural morphology of endosomal compartments after simultaneous depletion of all ESCRTs (Endosomal sorting complex required for transport). The brown colouring indicates enlarged, multivesicular endosomes (MVEs), which are still formed independently of ESCRT proteins. The red colour marks areas of endosomal membrane sheets that are docked together, displaying a typical, striated coat between the membrane layers. Within these membrane accumulations various smaller MVEs (cloured in green) are found. Mitochondria are marked in pink. From the article "Multivesicular endosome biogenesis in the absence of ESCRTs" by Stuffers S, Sem Wegner C, Stenmark H, Brech A., Traffic. 2009 Jul;10(7):925-37.
Cover of the Journal of Cell Science
Cover: HeLa cells treated with siRNA against Alix were stained for actin (red), cortactin (green) and the early-endosomal marker EEA1 (blue). Yellow colour shows colocalisation of actin and cortactin in abnormal actin structures. Article by Cabezas et al. ("Alix regulates cortical actin and the spatial distribution of endosomes" by Cabezas A, Bache KG, Brech A, Stenmark H., J Cell Sci. 2005 Jun 15;118(Pt 12):2625-35.)
Cover of Autophagy
From the article: "Promoting basal levels of autophagy in the nervous system enhances longevity and oxidant resistance in adult Drosophila" by Simonsen A, Cumming RC, Brech A, Isakson P, Schubert DR, Finley KD., Autophagy. 2008 Feb 16;4(2):176-84.
Cover of the Journal of Cell Biology
On the cover:
Protein deposits (red) are not cleaned up in autophagy-deficient cells. This might lead to dementia, say Filimenko et al.
See article: "Functional multivesicular bodies are required for autophagic clearance of protein aggregates associated with neurodegenerative disease" by Filimonenko M, Stuffers S, Raiborg C, Yamamoto A, Malerød L, Fisher EM, Isaacs A, Brech A, Stenmark H, Simonsen A., J Cell Biol. 2007 Nov 5;179(3):485-500.
Video and image gallery
EGF in HeLa cells.
Alexa488-labelled EGF was added to HeLa cells and internalized. The cells were then imaged by the Zeiss LSM Live scanner. Three-dimensional stacks were taken every minute. The green dots represent EGF in endosomes/lysosomes. The nucleus (in blue) is stained with Hoechst 33342. Made by Jørgen Wesche.
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HRS in microdomains on enlarged endosomes.
Three-dimensional reconstruction from image stacks of cells transfected with hrs-GFP. Hrs-GFP can be seen as microdomains on the surface of enlarged endosomes. The endosomes was enlarged by co-transfecting with constitutively active Rab5. Made by Camilla Raiborg and Jørgen Wesche.
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Internalization of EGF.
Fluorophore-labeled EGF was added to HeLa cells and the endocytosis was followed by time-lapse imaging for 20 minutes. The Zeiss confocal LSM510 was used for imaging. Made by Camilla Raiborg and Jørgen Wesche.
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Wound-healing assay.
After wounding, U2OS cells expressing FGF receptors were stimulated with FGF1 and monitored over time using the Nikon Biostation IM. Made by Angela Oppelt
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Nuclear import assay.
A NLS from FGF1 was fused to GFP and transfected into HeLa cells. The nucleus was bleached and the reappearance of green fluorescence into the nucleus indicates that the fusion protein is imported into the nucleus. Made by Jørgen Wesche.
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Internalization of anthrax toxin protective antigen (PA).
Fluorophore-labeled PA was added to HeLa cells and the intracellular transport was followed by time-lapse imaging. The Zeiss confocal LSM510 was used for imaging. Made by Jørgen Wesche.
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Internalization of anthrax toxin protective antigen (PA).
Fluorophore-labeled PA was added to HeLa cells and the intracellular transport was followed by time-lapse imaging. The Zeiss confocal LSM510 was used for imaging. Made by Jørgen Wesche.
Click here to play movie