Early stage ovarian carcinomas
=>About 25 % of all epithelial carcinomas are stage I, which means that disease is confined to the ovaries.
FIGO Stage I epithelial ovarian cancer:
5-year survival is about 80 %
Material
Methods
Patient characteristics
Conclusions
DNA ploidy by high-resolution image cytometry is the best predictor of outcome for patients with early stage carcinomas of the ovary, as compared to FIGO staging, histologic subtype, age, dense adhesions and differensiation.
High-resolution image cytometry is superior to flow cytometry in prediction of outcome for patients with early stage carcinomas of the ovary.
Table 1. Concordance between DNA ploidy classification performed by image and flow cytometry in 230 of the cases of early ovarian carcinoma.
Publications
FIGO Stage I epithelial ovarian cancer:
5-year survival is about 80 %
Material
- Retrospective study
- Paraffin embedded tissue sections
- A total of 305 cases of FIGO stage I epithelial ovarian cancers in Norway collected from 1982 to 1989
- A subgroup of 40 cases are selected from this material for immunohistochemical studies of carbohydrate expression (described later)
- Median follow up time is 9,5 years (range 0,8-12,5 years)
Methods
- DNA ploidy is determined using two different methods: flow cytometry (FCM) and image cytometry (ICM)
- Nucleotyping
- Immunohistochemistry
Patient characteristics
- FIGO stage IA 104, IB 19 and IC 182.
- Histological subtype: Serous 66, mucinous 80, endometroid 58, clear celled 69, mixed 10, unclassified 20 and small celled 2.
- Differentiation: Well 131; moderate 47; poor 58 and not graded 69.
Conclusions
DNA ploidy by high-resolution image cytometry is the best predictor of outcome for patients with early stage carcinomas of the ovary, as compared to FIGO staging, histologic subtype, age, dense adhesions and differensiation.
High-resolution image cytometry is superior to flow cytometry in prediction of outcome for patients with early stage carcinomas of the ovary.
| FCM \ ICM | Diploid | Tetraploid | Polyploid | Aneuploid | Sum |
| Diploid | 85 | 29 | 4 | 12 | 130 |
| Tetraploid | 12 | 4 | 15 | 31 | |
| Polyploid | 1 | 1 | |||
| Aneuploid | 9 | 4 | 1 | 54 | 68 |
| Sum | 94 | 45 | 10 | 81 | 230 |
Table 1. Concordance between DNA ploidy classification performed by image and flow cytometry in 230 of the cases of early ovarian carcinoma.
Publications