The iconic X shape of condensed mitotic chromosomes has en described in the 19th century, but we still know little about the structural organization of mitotic chromatin and its consequences on chromatin functions such as transcription. In this project we investigate mitotic chromatin architecture and its impact on transcription in Saccharomyces cerevisiae.
Mitotic poisons are used in the clinic against many cancers. However, they trigger deleterious side effects and their mechanisms of action remain unclear. There is therefore a need for a better understanding of mitotic events including mitotic gene expression. To address mitotic transcription we use high-throughput methods such as nascent RNA-seq, ChIP-seq or Hi-C and classical biochemistry.
Desumoylation of RNA polymerase III lies at the core of the Sumo stress response in yeast
J Biol Chem, 294 (49), 18784-18795
Corrigendum: Cdk1 gates cell cycle-dependent tRNA synthesis by regulating RNA polymerase III activity
Nucleic Acids Res, 46 (22), 12188-12189
Centromeres License the Mitotic Condensation of Yeast Chromosome Arms
Cell, 175 (3), 780-795.e15