SNPs with proven functional effect and clinical relevance for the response to the following anticancer drugs: 5-Fluorouracyl, Doxorubicin, Metothrexate, Etoposide, 6-Mercaptopurine, Azothiopurin, Thioguanine, Irinotekan are studied in this project. The high precision genotyping of these SNPs is performed on the Nanogen platform. Genotyping on the Nanogen platform involves attachment of a biotinylated PCR product to a chip coated with hydrogel enriched with streptavidin, which is addressed by a stabilizer and a fluorescent labelled probe reporting for each of the variant alleles.
Figure A. Principle of SNP detection with the Nanogen system. Genotyping involves attaching a biotinylated PCR product to a streptavidin coated chip. A fluorescent probe that reports the respective allele is addressed to the amplicon. Alternatively, the probe can be attached to the solid surface and addressed by the PCR product (click to enlarge).
Key members: Silje Nordgard og Grethe I. Grenaker Alnæs
Akman T, Arendt LM, Geisler J, Kristensen VN, Frigessi A, Köhn-Luque A(2024) Modeling of Mouse Experiments Suggests that Optimal Anti-Hormonal Treatment for Breast Cancer is Diet-Dependent Bull Math Biol, 86(4), 42 DOI 10.1007/s11538-023-01253-1, PubMed 38498130
Yiangou K, Mavaddat N, Dennis J, Zanti M, Wang Q, Bolla MK, Abubakar M, Ahearn TU, Andrulis IL, Anton-Culver H, Antonenkova NN, Arndt V, Aronson KJ, Augustinsson A, Baten A, Behrens S, Bermisheva M, de Gonzalez AB, Białkowska K, Boddicker N, Bodelon C, Bogdanova NV, Bojesen SE, Brantley KD, Brauch Het al.(2024) Differences in polygenic score distributions in European ancestry populations: implications for breast cancer risk prediction medRxiv DOI 10.1101/2024.02.12.24302043, PubMed 38410445
Tinholt M, Tekpli X, Torland LA, Tahiri A, Geisler J, Kristensen V, Sandset PM, Iversen N(2024) The breast cancer coagulome in the tumor microenvironment and its role in prognosis and treatment response to chemotherapy J Thromb Haemost(in press) DOI 10.1016/j.jtha.2024.01.003, PubMed 38237862
