| Håvard K. Skjellegrind | |
| Position: | PhD student, MD |
| Phone: | +47 23071394 |
| Email: | |
Live imaging of developing cells in vitro
The aim of this project is to develop live cell imaging of neural stem cells and tumor forming cells.
Subsidiary aims
Detailed recording of cell behaviour in culture using timelapse imaging will reveal insights into cell biology, differentiation and acquisition of function.
Methods
Comparison between normal and cancer tissue is a means to unearth differences in their respective biologies. As well we aim to develop cell replacement therapy using stem cells from neural tissue. Detailed recording of cell behaviour in culture using timelapse imaging will reveal insights into cell biology, differentiation and acquisition of function. Cells will be labeled with genetically driven reporter constructs (another project) and the information provided by filmed movements and colour fluorescence expression will reveal insights into developmental events involved in both normal and tumor development.
Scientific impact
The lab has now established capability of picking single cells by micromanipulator and processing these single cells for limited gene expression arrays. This ability gives us a new level of precision with regards to understanding cell biology. We can now look at the behaviour of single cells. In hand with this capability now comes the need to track and visualise these same cells before molecular analysis. If, for example we can film a cell’s movements, interactions and subsequent display of green fluorescence as it changes into a specific type of neuron we will have made a quantum leap in the precision with which we can analyse neurodevelopmental events.
Subsidiary aims
Detailed recording of cell behaviour in culture using timelapse imaging will reveal insights into cell biology, differentiation and acquisition of function.
Methods
Comparison between normal and cancer tissue is a means to unearth differences in their respective biologies. As well we aim to develop cell replacement therapy using stem cells from neural tissue. Detailed recording of cell behaviour in culture using timelapse imaging will reveal insights into cell biology, differentiation and acquisition of function. Cells will be labeled with genetically driven reporter constructs (another project) and the information provided by filmed movements and colour fluorescence expression will reveal insights into developmental events involved in both normal and tumor development.
Scientific impact
The lab has now established capability of picking single cells by micromanipulator and processing these single cells for limited gene expression arrays. This ability gives us a new level of precision with regards to understanding cell biology. We can now look at the behaviour of single cells. In hand with this capability now comes the need to track and visualise these same cells before molecular analysis. If, for example we can film a cell’s movements, interactions and subsequent display of green fluorescence as it changes into a specific type of neuron we will have made a quantum leap in the precision with which we can analyse neurodevelopmental events.
Preliminary results
Shown videos are tumor stem cells exposed to a differentiation stimulating factor and control. The videos are filmed simultaneously using a programmed motorized stage on an Olympus IX81 microscope.
Copyright Vilhelm Magnus Center 2010
Stimulated | Control |
Copyright Vilhelm Magnus Center 2010
Author network for Håvard K. Skjellegrind by COREMINE medical
Publications 2008
Mitochondria are more resistant to hypoxic depolarization in the newborn than in the adult brain
Neurochem Res, 33 (9), 1894-900
PubMed 18363097
Publications 2006
Depolarization of mitochondria in isolated CA1 neurons during hypoxia, glucose deprivation and glutamate excitotoxicity
Brain Res, 1077 (1), 153-60
PubMed 16480964
Publications 2005
Endoplasmic reticulum dysfunction and Ca2+ deregulation in isolated CA1 neurons during oxygen and glucose deprivation
Neurochem Res, 30 (5), 651-9
PubMed 16176069
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