Per Olaf Ekstrøm

Jul. 95 - Sept. 97 Ph.D The medical faculty, Universities of Oslo (UiO)
Jan. 94 - Jun. 95 MSc. Toxicology, Biological institute, UiO
Aug. 88 - Jan. 91 BSc, Biology, UiO
Aug. 87 - Jun. 88 Master of Business Administration program (MBA), BI Norwegian School of Management.
Sept. 85 - April 86 “Petty officer”. Officers training for the coast artillery
Aug. 81 - Jun. 84 High school. Berg videregående skole

Work experiences
Oct. 99 –> Head of Clinical Research Department of Surgical Oncology,
The Norwegian Radium hospital HF (DNR) HF
Oct. 97 – Sept.99 Post Doctoral associate at Center for Environmental Health Sciences, Massachusetts Institute of Technology (MIT), Cambridge, USA
Jan. 97 - Sept.97 PhD candidate, Department of Surgical Oncology,
The Norwegian Radium hospital (DNR)
Jan. 96 - Des 96 Research Assistant,
Department of Surgical Oncology, (DNR)
Jan. 94 - Des 95 50% Position as Research Assistant,
Department of Surgical Oncology, (DNR)
Mars 91 - Des 93 Research Assistant,
Department of Surgical Oncology, (DNR)
May 86 - Nov. 86 Acting “Petty officer” coastal artillery base Northern Norway

Doktorgradens tittel (Ph.D.)



· Supervised Jens Bjørheim for PhD thesis summer 2003
· Supervision of Post Doc Jens Bjørheim, PhD. Candidate Annette Kristensen.
· Daily responsibility for technical personal, Ms Abrahamsen and Ms Heintz
· Responsible for DNA sequencing core facility

Courses and Conferences

Targeted Cancer Therapies, Tromsø Symposium

5th International Meeting On Single Nucleotide Polymorphism And Complex Genome Analysis Reykjavik, Iceland, Approach To Analysis of SNPs In Populations
Watrex MegaBACE user's meeting, Poster and oral presentation., Prague, Czech Republic.

GSAC 2001, 13th International Genome Sequencing and Analysis Conference, SNP Detection by Melting Heteroduplex Analysis using MegaBACETM 96-capillary Array Genetic Analyzer

ASHG 2001, American Society of Human Gentics
High-Throughput Mutation Detection And Screening Using Megabace™ Capillary Array Instrument For Genetic Analysis.

ICHG 2001, 10th International Congress of Human Genetics
High-throughput Mutation Detection and Screening by Constant Denaturant Capillary Array Electrophoresis Megabace™ Capillary Array Instrument For Genetic Analysis.

Bloodborne pathogen, Single Nucleotide Polymorphisms-project, MIT

"Tox.Talk", oral presentation of project, MIT
Mechanical engineering workshop safety training, 
Waste management and laboratory safety, MIT

Laser safety course, MIT
Oral presentation symposium in Tromsø. Tittel; "Preoperative mutation analysis - wishful thinking or future reality?"
Symposium in radiobiology, Rosendal
Symposium in clinical microdialysis, Uppsala, Sverige
Basic training in medical research, the medical faculty, University of Bergen

Certificate for handling laboratory animals, Approved by the Agriculture department, category C - researcher
Oral presentation on microdialysis users meeting in Stockholm, Sweden
Seminar in scientific theory, the medical faculty, UiO
Research collaboration and technology transfer between the Norwegian Radium Hospital, Center for Nutrition and Toxicology, Karolinska Institute and Center for Environmental Health Sciences, MIT
Upgrading of Constant Denaturant Capillary Electrophoresis (CDCE) instrument at Center for Environmental Health Sciences, MIT

Basic course in nuclear medicine. Norwegian Medical Association 
Seventh International Symposium on High Performance Capillary Electrophoresis, Würzburg, Tyskland

Basic introductions to the theory of Constant Denaturant Capillary Electrophoresis (CDCE), Center for Environmental Health Sciences, Massachusetts Institute of Technology (MIT)
Supervisor seminar, education department, DNR

International Symposium on Microdialysis and Allied Analytical Techniques, Indianapolis, USA
Seminar in presentation techniques, education department, DNR

Basic microdialysis training, CMA/Microdialysis, Stockholm, Sweden


Author network for Per Olaf Ekstrøm by COREMINE medical

Publications 2017

Arstad C, Refinetti P, Kristensen AT, Giercksky KE, Ekstrøm PO (2017)
Is detection of intraperitoneal exfoliated tumor cells after surgical resection of rectal cancer a prognostic factor of survival?
BMC Cancer, 17 (1), 406
PubMed 28592327

Dominguez-Valentin M, Nakken S, Tubeuf H, Vodak D, Ekstrøm PO, Nissen AM, Morak M, Holinski-Feder E, Martins A, Møller P, Hovig E (2017)
Potentially pathogenic germline CHEK2 c.319+2T>A among multiple early-onset cancer families
Fam Cancer (in press)
PubMed 28608266

Refinetti P, Arstad C, Thilly WG, Morgenthaler S, Ekstrøm PO (2017)
Mapping mitochondrial heteroplasmy in a Leydig tumor by laser capture micro-dissection and cycling temperature capillary electrophoresis
BMC Clin Pathol, 17, 6
PubMed 28405177

Publications 2016

Arstad C, Refinetti P, Warren D, Giercksky KE, Ekstrøm PO (2016)
Scanning the mitochondrial genome for mutations by cycling temperature capillary electrophoresis
Mitochondrial DNA A DNA Mapp Seq Anal, 1-12 (in press)
PubMed 27728990

Joshi S, Schjølberg AR, Ekstrøm PO, De Angelis PM, Zucknick M, Andersen SN, Clausen OP (2016)
Tp53/p53 status in keratoacanthomas
J Cutan Pathol, 43 (7), 571-8
PubMed 27020606

Refinetti P, Morgenthaler S, Ekstrøm PO (2016)
Cycling temperature capillary electrophoresis: A quantitative, fast and inexpensive method to detect mutations in mixed populations of human mitochondrial DNA
Mitochondrion, 29, 65-74
PubMed 27166160

Publications 2015

Ekstrøm PO, Nakken S, Johansen M, Hovig E (2015)
Automated amplicon design suitable for analysis of DNA variants by melting techniques
BMC Res Notes, 8, 667
PubMed 26559640

Heramb C, Ekstrøm PO, Tharmaratnam K, Hovig E, Møller P, Mæhle L (2015)
Ten modifiers of BRCA1 penetrance validated in a Norwegian series
Hered Cancer Clin Pract, 13 (1), 14
PubMed 26052370

Publications 2014

Friis-Ottessen M, Burum-Auensen E, Schjølberg AR, Ekstrøm PO, Andersen SN, Clausen OP, De Angelis PM (2014)
TP53/p53 alterations and Aurora A expression in progressor and non-progressor colectomies from patients with longstanding ulcerative colitis
Int J Mol Med, 35 (1), 24-30
PubMed 25333414

Publications 2013

Yri OE, Ekstrøm PO, Hilden V, Gaudernack G, Liestøl K, Smeland EB, Holte H (2013)
Influence of polymorphisms in genes encoding immunoregulatory proteins and metabolizing enzymes on susceptibility and outcome in patients with diffuse large B-cell lymphoma treated with rituximab
Leuk Lymphoma, 54 (10), 2205-14
PubMed 23391141

Publications 2012

Ekstrøm PO, Warren DJ, Thilly WG (2012)
Separation principles of cycling temperature capillary electrophoresis
Electrophoresis, 33 (7), 1162-8
PubMed 22539319

Yri OE, Ekstrøm PO, Hilden V, Gaudernack G, Liestøl K, Smeland EB, Holte H (2012)
Polymorphisms in genes encoding interleukin-10 and drug metabolizing enzymes GSTP1, GSTT1, GSTA1 and UGT1A1 influence risk and outcome in Hodgkin lymphoma
Leuk Lymphoma, 53 (10), 1934-44
PubMed 22475179

Publications 2010

Sørensen O, Andersen A, Olsen H, Alexandr K, Ekstrøm PO, Giercksky KE, Flatmark K (2010)
Validation and use of microdialysis for determination of pharmacokinetic properties of the chemotherapeutic agent mitomycin C - an experimental study
BMC Cancer, 10, 469
PubMed 20809961

Publications 2008

Ekstrøm PO (2008)
Scanning for DNA variants by denaturant capillary electrophoresis
Methods Mol Biol, 439, 19-34
PubMed 18370093

Ekstrøm PO, Khrapko K, Li-Sucholeiki XC, Hunter IW, Thilly WG (2008)
Analysis of mutational spectra by denaturing capillary electrophoresis
Nat Protoc, 3 (7), 1153-66
PubMed 18600220

Kristensen AT, Wiig JN, Larsen SG, Giercksky KE, Ekstrøm PO (2008)
Molecular detection (k-ras) of exfoliated tumour cells in the pelvis is a prognostic factor after resection of rectal cancer?
BMC Cancer, 8, 213
PubMed 18655729

Sudo H, Li-Sucholeiki XC, Marcelino LA, Gruhl AN, Herrero-Jimenez P, Zarbl H, Willey JC, Furth EE, Morgenthaler S, Coller HA, Ekstrom PO, Kurzweil R, Gostjeva EV, Thilly WG (2008)
Fetal-juvenile origins of point mutations in the adult human tracheal-bronchial epithelium: absence of detectable effects of age, gender or smoking status
Mutat Res, 646 (1-2), 25-40
PubMed 18824180

Publications 2007

Ekstrøm PO, Bjørheim J, Thilly WG (2007)
Technology to accelerate pangenomic scanning for unknown point mutations in exonic sequences: cycling temperature capillary electrophoresis (CTCE)
BMC Genet, 8, 54
PubMed 17697348

Lind H, Ekstrøm PO, Ryberg D, Skaug V, Andreassen T, Stangeland L, Haugen A, Zienolddiny S (2007)
Frequency of TP53 mutations in relation to Arg72Pro genotypes in non small cell lung cancer
Cancer Epidemiol Biomarkers Prev, 16 (10), 2077-81
PubMed 17932356

Publications 2006

Ekstrøm PO, Bjørheim J (2006)
Evaluation of sieving matrices used to separate alleles by cycling temperature capillary electrophoresis
Electrophoresis, 27 (10), 1878-85
PubMed 16619298

Harbo HF, Ekstrøm PO, Lorentzen AR, Sundvold-Gjerstad V, Celius EG, Sawcer S, Spurkland A (2006)
Coding region polymorphisms in T cell signal transduction genes. Prevalence and association to development of multiple sclerosis
J Neuroimmunol, 177 (1-2), 40-5
PubMed 16764945

Lind H, Zienolddiny S, Ekstrøm PO, Skaug V, Haugen A (2006)
Association of a functional polymorphism in the promoter of the MDM2 gene with risk of nonsmall cell lung cancer
Int J Cancer, 119 (3), 718-21
PubMed 16496380

Publications 2005

Bjørheim J, Ekstrøm PO (2005)
Review of denaturant capillary electrophoresis in DNA variation analysis
Electrophoresis, 26 (13), 2520-30
PubMed 15934053

Hinselwood DC, Abrahamsen TW, Ekstrøm PO (2005)
BRAF mutation detection and identification by cycling temperature capillary electrophoresis
Electrophoresis, 26 (13), 2553-61
PubMed 15948220

Hinselwood DC, Warren DJ, Ekstrøm PO (2005)
High-throughput gender determination using automated denaturant gel capillary electrophoresis
Electrophoresis, 26 (13), 2562-6
PubMed 15934052

Li-Sucholeiki XC, Tomita-Mitchell A, Arnold K, Glassner BJ, Thompson T, Murthy JV, Berk L, Lange C, Leong-Morgenthaler PM, MacDougall D, Munro J, Cannon D, Mistry T, Miller A, Deka C, Karger B, Gillespie KM, Ekstrøm PO, Todd JA, Thilly WG (2005)
Detection and frequency estimation of rare variants in pools of genomic DNA from large populations using mutational spectrometry
Mutat Res, 570 (2), 267-80
PubMed 15708585

Lorentzen AR, Celius EG, Ekstrøm PO, Wiencke K, Lie BA, Myhr KM, Ling V, Thorsby E, Vartdal F, Spurkland A, Harbo HF (2005)
Lack of association with the CD28/CTLA4/ICOS gene region among Norwegian multiple sclerosis patients
J Neuroimmunol, 166 (1-2), 197-201
PubMed 16005527

Publications 2004

Ekstrøm PO, Bjørge T, Dørum A, Longva AS, Heintz KM, Warren DJ, Hansen S, Gislefoss RE, Hovig E (2004)
Determination of hereditary mutations in the BRCA1 gene using archived serum samples and capillary electrophoresis
Anal Chem, 76 (15), 4406-9
PubMed 15283579

Publications 2003

Bjørheim J, Abrahamsen TW, Kristensen AT, Gaudernack G, Ekstrøm PO (2003)
Approach to analysis of single nucleotide polymorphisms by automated constant denaturant capillary electrophoresis
Mutat Res, 526 (1-2), 75-83
PubMed 12714185

Bjørheim J, Gaudernack G, Giercksky KE, Ekstrøm PO (2003)
Direct identification of all oncogenic mutants in KRAS exon 1 by cycling temperature capillary electrophoresis
Electrophoresis, 24 (1-2), 63-9
PubMed 12652573

Kristensen AT, Bjørheim J, Wiig J, Giercksky KE, Ekstrøm PO (2003)
DNA variants in the ATM gene are not associated with sporadic rectal cancer in a Norwegian population-based study
Int J Colorectal Dis, 19 (1), 49-54
PubMed 12827413

Minarik M, Minarikova L, Bjørheim J, Ekstrøm PO (2003)
Cycling gradient capillary electrophoresis: a low-cost tool for high-throughput analysis of genetic variations
Electrophoresis, 24 (11), 1716-22
PubMed 12783447

Publications 2002

Bjørheim J, Minarik M, Gaudernack G, Ekstrøm PO (2002)
Mutation detection in KRAS Exon 1 by constant denaturant capillary electrophoresis in 96 parallel capillaries
Anal Biochem, 304 (2), 200-5
PubMed 12009696

Ekstrøm PO, Bjørheim J (2002)
Reducing the cost of consumables used for ABI 310 genetic analyzer
Anal Biochem, 306 (1), 148-9
PubMed 12069426

Ekstrøm PO, Bjørheim J, Gaudernack G, Giercksky KE (2002)
Population screening of single-nucleotide polymorphisms exemplified by analysis of 8000 alleles
J Biomol Screen, 7 (6), 501-6
PubMed 14599347

Kristensen AT, Bjørheim J, Ekstrøm PO (2002)
Detection of mutations in exon 8 of TP53 by temperature gradient 96-capillary array electrophoresis
Biotechniques, 33 (3), 650-3
PubMed 12238774

Publications 2001

Bjørheim J, Ekstrøm PO, Fossberg E, Børresen-Dale AL, Gaudernack G (2001)
Automated constant denaturant capillary electrophoresis applied for detection of KRAS exon 1 mutations
Biotechniques, 30 (5), 972-5
PubMed 11355359

Bjørheim J, Gaudernack G, Ekstrøm PO (2001)
Mutation analysis of TP53 exons 5-8 by automated constant denaturant capillary electrophoresis
Tumour Biol, 22 (5), 323-7
PubMed 11553863

Publications 2000

Ekstrøm PO, Wasserkort R, Minarik M, Foret F, Thilly WG (2000)
Two-point fluorescence detection and automated fraction collection applied to constant denaturant capillary electrophoresis
Biotechniques, 29 (3), 582-4, 586-9
PubMed 10997272

Publications 1999

Ekstrøm PO, Børresen-Dale AL, Qvist H, Giercksky KE, Thilly WG (1999)
Detection of low-frequency mutations in exon 8 of the TP53 gene by constant denaturant capillary electrophoresis (CDCE)
Biotechniques, 27 (1), 128-34
PubMed 10407675

Publications 1997

Ekstrøm PO, Andersen A, Saeter G, Giercksky KE, Slørdal L (1997)
Continuous intratumoral microdialysis during high-dose methotrexate therapy in a patient with malignant fibrous histiocytoma of the femur: a case report
Cancer Chemother Pharmacol, 39 (3), 267-72
PubMed 8996531

Ekstrøm PO, Giercksky KE, Andersen A, Bruland OS, Slørdal L (1997)
Intratumoral differences in methotrexate levels within human osteosarcoma xenografts studied by microdialysis
Life Sci, 61 (19), PL275-80
PubMed 9364204

Ekstrøm PO, Giercksky KE, Andersen A, Slørdal L (1997)
Alterations in methotrexate pharmacokinetics by naproxen in the rat as measured by microdialysis
Life Sci, 60 (24), PL 359-64
PubMed 9188769

Publications 1996

Ekstrøm PO, Andersen A, Warren DJ, Giercksky KE, Slørdal L (1996)
Determination of extracellular methotrexate tissue levels by microdialysis in a rat model
Cancer Chemother Pharmacol, 37 (5), 394-400
PubMed 8599860

Publications 1995

Ekstrøm PO, Anderson A, Warren DJ, Giercksky KE, Slørdal L (1995)
Pharmacokinetics of different doses of methotrexate at steady state by in situ microdialysis in a rat model
Cancer Chemother Pharmacol, 36 (4), 283-9
PubMed 7628046